pLEXX-AK

Bacterial vector for simultaneous cloning of two DNA fragments in the ClonePlex® library construction system.

Sequence Author: Lucigen

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets
No matches
BstXI (2885) BfuAI - BspMI (2862) KanR1 Primer (2838 .. 2859) PflMI (2678) BsmBI (2431) AsiSI (2415) SspI (2340) EcoNI (2327) PasI (2287) EarI (2228) NruI (2072) AvaI - BmeT110I - BsoBI - PaeR7I - PspXI - XhoI (2013) BspHI (1933) KanL1 Primer (1897 .. 1920) PmeI (1877) BamHI (1845) HincII (1833) SL1 Primer (1775 .. 1795) AlwNI (1478) HincII (0) XbaI (10) SwaI (37) SR2 Primer (33 .. 55) tonB terminator BsaAI (106) XmnI (387) BsaHI (447) TatI (504) ScaI (506) EaeI (594) XcmI (604) FspI (764) NmeAIII (840) BglI (869) BsaI (921) BmrI (947) AhdI (987) EcoO109I - PspOMI (1064) ApaI (1068) BciVI (1265) BseYI (1366) PspFI (1370) pLEXX-AK™ 2891 bp
BstXI  (2885)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BfuAI  (2862)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (2862)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PflMI  (2678)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BsmBI  (2431)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
AsiSI  (2415)
1 site
G C G A T C G C C G C T A G C G
SspI  (2340)
1 site
A A T A T T T T A T A A
EcoNI  (2327)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PasI  (2287)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
EarI  (2228)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
NruI  (2072)
1 site
T C G C G A A G C G C T
AvaI  (2013)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BmeT110I  (2013)
1 site
C Y C G R G G R G C Y C
BsoBI  (2013)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (2013)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (2013)
1 site
V C T C G A G B B G A G C T C V
XhoI  (2013)
1 site
C T C G A G G A G C T C
BspHI  (1933)
1 site
T C A T G A A G T A C T
PmeI  (1877)
1 site
G T T T A A A C C A A A T T T G
BamHI  (1845)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
HincII  (1833)
2 sites
G T Y R A C C A R Y T G
AlwNI  (1478)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
HincII  (0)
2 sites
G T Y R A C C A R Y T G
XbaI  (10)
1 site
T C T A G A A G A T C T
SwaI  (37)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
BsaAI  (106)
1 site
Y A C G T R R T G C A Y
XmnI  (387)
1 site
G A A N N N N T T C C T T N N N N A A G
BsaHI  (447)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
TatI  (504)
1 site
W G T A C W W C A T G W
ScaI  (506)
1 site
A G T A C T T C A T G A
EaeI  (594)
1 site
Y G G C C R R C C G G Y
XcmI  (604)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
FspI  (764)
1 site
T G C G C A A C G C G T
NmeAIII  (840)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (869)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsaI  (921)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BmrI  (947)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
AhdI  (987)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
EcoO109I  (1064)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PspOMI  (1064)
1 site
G G G C C C C C C G G G
ApaI  (1068)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BciVI  (1265)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BseYI  (1366)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PspFI  (1370)
1 site
C C C A G C G G G T C G
KanR1 Primer
22-mer  /  32% GC
1 binding site
2838 .. 2859  =  22 annealed bases
Tm  =  52°C
KanL1 Primer
24-mer  /  46% GC
1 binding site
1897 .. 1920  =  24 annealed bases
Tm  =  60°C
SL1 Primer
21-mer  /  57% GC
1 binding site
1775 .. 1795  =  21 annealed bases
Tm  =  59°C
SR2 Primer
23-mer  /  39% GC
1 binding site
33 .. 55  =  23 annealed bases
Tm  =  54°C
AmpR
200 .. 1060  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   200 .. 268  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
200 .. 1060  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   269 .. 1060  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
200 .. 1060  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
KanR
1984 .. 2799  =  816 bp
271 amino acids  =  30.9 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
KanR
1984 .. 2799  =  816 bp
271 amino acids  =  30.9 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin
ori
1123 .. 1710  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1123 .. 1710  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
cat promoter
97 .. 199  =  103 bp
promoter of the E. coli cat gene
cat promoter
97 .. 199  =  103 bp
promoter of the E. coli cat gene
rrnB T1 terminator
2801 .. 2846  =  46 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
2801 .. 2846  =  46 bp
transcription terminator T1 from the E. coli rrnB gene
tonB terminator
65 .. 96  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
tonB terminator
65 .. 96  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
T3Te terminator
1732 .. 1761  =  30 bp
phage T3 early transcription terminator
T3Te terminator
1732 .. 1761  =  30 bp
phage T3 early transcription terminator
T7Te terminator
1084 .. 1111  =  28 bp
phage T7 early transcription terminator
T7Te terminator
1084 .. 1111  =  28 bp
phage T7 early transcription terminator
ORF:  1984 .. 2799  =  816 bp
ORF:  271 amino acids  =  30.9 kDa
ORF:  200 .. 1060  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  2594 .. 35  =  333 bp
ORF:  110 amino acids  =  12.9 kDa
ORF:  664 .. 966  =  303 bp
ORF:  100 amino acids  =  10.5 kDa
Click here to try SnapGene

Download pLEXX-AK.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.