pMMB190

Broad-host-range vector with an ampicillin resistance gene, suitable for blue-white screening and expression of toxic proteins.
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EcoRI (9064) lac operator lac operator tac promoter MauBI (8688) MluI (8231) ApaI - BanII (8028) PspOMI (8024) HpaI (7729) BsmBI - Esp3I (7616) BspQI - SapI (7423) FspAI (6952) BsaAI (6897) EcoNI (6802) PfoI * (6662) SgrAI (4714) TspMI - XmaI (3) SmaI (5) BamHI (8) SalI (14) PstI (24) HindIII (28) rrnB T2 terminator BssSI - BssSαI (827) DraI (918) AhdI (1496) CsiI - SexAI * (1620) SfiI (1854) EcoO109I (2406) BstZ17I (3229) PasI (4235) pMMB190 9066 bp
EcoRI  (9064)
1 site
G A A T T C C T T A A G
MauBI  (8688)
1 site
C G C G C G C G G C G C G C G C
MluI  (8231)
1 site
A C G C G T T G C G C A
ApaI  (8028)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (8028)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
PspOMI  (8024)
1 site
G G G C C C C C C G G G
HpaI  (7729)
1 site
G T T A A C C A A T T G
BsmBI  (7616)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (7616)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
BspQI  (7423)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (7423)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
FspAI  (6952)
1 site
R T G C G C A Y Y A C G C G T R
BsaAI  (6897)
1 site
Y A C G T R R T G C A Y
EcoNI  (6802)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PfoI  (6662)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
SgrAI  (4714)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
TspMI  (3)
1 site
C C C G G G G G G C C C
XmaI  (3)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (5)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (8)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SalI  (14)
1 site
G T C G A C C A G C T G
PstI  (24)
1 site
C T G C A G G A C G T C
HindIII  (28)
1 site
A A G C T T T T C G A A
BssSI  (827)
1 site
C A C G A G G T G C T C
BssSαI  (827)
1 site
C A C G A G G T G C T C
DraI  (918)
1 site
T T T A A A A A A T T T
AhdI  (1496)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
CsiI  (1620)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (1620)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SfiI  (1854)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
EcoO109I  (2406)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BstZ17I  (3229)
1 site
G T A T A C C A T A T G
PasI  (4235)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
lacI
7504 .. 8586  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
7504 .. 8586  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RSF1010 RepB
4068 .. 5039  =  972 bp
323 amino acids  =  35.9 kDa
Product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepB
4068 .. 5039  =  972 bp
323 amino acids  =  35.9 kDa
Product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
AmpR
709 .. 1569  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   709 .. 777  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
709 .. 1569  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   778 .. 1569  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
709 .. 1569  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
RSF1010 RepC
6376 .. 7227  =  852 bp
283 amino acids  =  31.1 kDa
Product: replication protein C of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepC
6376 .. 7227  =  852 bp
283 amino acids  =  31.1 kDa
Product: replication protein C of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 oriV
2006 .. 2400  =  395 bp
replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)
RSF1010 oriV
2006 .. 2400  =  395 bp
replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)
lacZα
9050 .. 208  =  225 bp
74 amino acids  =  8.2 kDa
Product: LacZα fragment of β-galactosidase
lacZα
9050 .. 208  =  225 bp
74 amino acids  =  8.2 kDa
Product: LacZα fragment of β-galactosidase
AmpR promoter
617 .. 708  =  92 bp
AmpR promoter
617 .. 708  =  92 bp
RSF1010 oriT
2742 .. 2829  =  88 bp
origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 oriT
2742 .. 2829  =  88 bp
origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
rrnB T1 terminator
393 .. 479  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
393 .. 479  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
lacIq promoter
8587 .. 8664  =  78 bp
In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.
lacIq promoter
8587 .. 8664  =  78 bp
In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.
lac promoter
8976 .. 9006  =  31 bp
3 segments
   Segment 1:  -35  
   8976 .. 8981  =  6 bp
promoter for the E. coli lac operon
lac promoter
8976 .. 9006  =  31 bp
3 segments
   Segment 2:  
   8982 .. 8999  =  18 bp
promoter for the E. coli lac operon
lac promoter
8976 .. 9006  =  31 bp
3 segments
   Segment 3:  -10  
   9000 .. 9006  =  7 bp
promoter for the E. coli lac operon
lac promoter
8976 .. 9006  =  31 bp
3 segments
promoter for the E. coli lac operon
tac promoter
8894 .. 8922  =  29 bp
3 segments
   Segment 1:  -35  
   8894 .. 8899  =  6 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
8894 .. 8922  =  29 bp
3 segments
   Segment 2:  
   8900 .. 8915  =  16 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
8894 .. 8922  =  29 bp
3 segments
   Segment 3:  -10  
   8916 .. 8922  =  7 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
8894 .. 8922  =  29 bp
3 segments
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
rrnB T2 terminator
571 .. 598  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
rrnB T2 terminator
571 .. 598  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
lac operator
8930 .. 8946  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
8930 .. 8946  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
9014 .. 9030  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
9014 .. 9030  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RSF1010 RepA
5550 .. 6389  =  840 bp
279 amino acids  =  29.9 kDa
Product: replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepA
5550 .. 6389  =  840 bp
279 amino acids  =  29.9 kDa
Product: replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
MCS
9064 .. 33  =  36 bp
multiple cloning site
MCS
9064 .. 33  =  36 bp
multiple cloning site
M13 fwd
37 .. 53  =  17 bp
common sequencing primer, one of multiple similar variants
M13 fwd
37 .. 53  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
9038 .. 9054  =  17 bp
common sequencing primer, one of multiple similar variants
M13 rev
9038 .. 9054  =  17 bp
common sequencing primer, one of multiple similar variants
ORF:  709 .. 1569  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  2206 .. 2634  =  429 bp
ORF:  142 amino acids  =  15.3 kDa
ORF:  2752 .. 3159  =  408 bp
ORF:  135 amino acids  =  14.3 kDa
ORF:  3658 .. 4071  =  414 bp
ORF:  137 amino acids  =  15.1 kDa
ORF:  4495 .. 5139  =  645 bp
ORF:  214 amino acids  =  22.8 kDa
ORF:  6277 .. 7227  =  951 bp
ORF:  316 amino acids  =  34.4 kDa
ORF:  1667 .. 1999  =  333 bp
ORF:  110 amino acids  =  12.4 kDa
ORF:  9050 .. 208  =  225 bp
ORF:  74 amino acids  =  8.2 kDa
ORF:  2910 .. 5039  =  2130 bp
ORF:  709 amino acids  =  78.0 kDa
ORF:  5550 .. 6389  =  840 bp
ORF:  279 amino acids  =  29.9 kDa
ORF:  8977 .. 312  =  402 bp
ORF:  133 amino acids  =  14.7 kDa
ORF:  1588 .. 1866  =  279 bp
ORF:  92 amino acids  =  10.3 kDa
ORF:  6610 .. 6948  =  339 bp
ORF:  112 amino acids  =  12.1 kDa
ORF:  7504 .. 8463  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
ORF:  234 .. 560  =  327 bp
ORF:  108 amino acids  =  12.1 kDa
ORF:  1173 .. 1439  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2427 .. 2711  =  285 bp
ORF:  94 amino acids  =  10.9 kDa
ORF:  2781 .. 3437  =  657 bp
ORF:  218 amino acids  =  22.9 kDa
ORF:  3630 .. 3968  =  339 bp
ORF:  112 amino acids  =  12.0 kDa
ORF:  4185 .. 4778  =  594 bp
ORF:  197 amino acids  =  20.5 kDa
ORF:  5547 .. 6314  =  768 bp
ORF:  255 amino acids  =  27.0 kDa
ORF:  6717 .. 7058  =  342 bp
ORF:  113 amino acids  =  12.6 kDa
ORF:  1556 .. 1873  =  318 bp
ORF:  105 amino acids  =  12.2 kDa
ORF:  3077 .. 3307  =  231 bp
ORF:  76 amino acids  =  8.4 kDa
ORF:  3974 .. 4327  =  354 bp
ORF:  117 amino acids  =  12.6 kDa
ORF:  5801 .. 6166  =  366 bp
ORF:  121 amino acids  =  13.3 kDa
ORF:  7484 .. 7747  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  8336 .. 8653  =  318 bp
ORF:  105 amino acids  =  11.2 kDa
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Download pMMB190.dna file

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Individual Sequences & Maps

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