pMMB207

Broad-host-range bacterial cloning vector with the pUC18/19 MCS and a chloramphenicol resistance gene. See also pMMB208.
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets
No matches
HindIII (9046) SphI (9044) PstI - SbfI (9038) SfcI (9034) SalI (9028) XbaI (9022) BamHI (9016) SmaI (9013) KpnI - TspMI - XmaI (9011) Acc65I (9007) SacI (9005) Eco53kI (9003) EcoRI (8995) lac operator tac promoter MauBI (8720) MluI (8263) ApaI (8060) PspOMI (8056) HpaI (7761) BspQI - SapI (7455) FspAI (6984) EcoNI (6834) PfoI * (6694) SgrAI (4746) XmnI (0) BsaI (148) rrnB T1 terminator rrnB T2 terminator BssSI - BssSαI (612) BspEI (1256) SnaBI (1266) SfiI (1886) EcoO109I (2438) BstZ17I (3261) pMMB207 9076 bp
HindIII  (9046)
1 site
A A G C T T T T C G A A
SphI  (9044)
1 site
G C A T G C C G T A C G
PstI  (9038)
1 site
C T G C A G G A C G T C
SbfI  (9038)
1 site
C C T G C A G G G G A C G T C C
SfcI  (9034)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SalI  (9028)
1 site
G T C G A C C A G C T G
XbaI  (9022)
1 site
T C T A G A A G A T C T
BamHI  (9016)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (9013)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
KpnI  (9011)
1 site
G G T A C C C C A T G G
TspMI  (9011)
1 site
C C C G G G G G G C C C
XmaI  (9011)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
Acc65I  (9007)
1 site
G G T A C C C C A T G G
SacI  (9005)
1 site
G A G C T C C T C G A G
Eco53kI  (9003)
1 site
G A G C T C C T C G A G
EcoRI  (8995)
1 site
G A A T T C C T T A A G
MauBI  (8720)
1 site
C G C G C G C G G C G C G C G C
MluI  (8263)
1 site
A C G C G T T G C G C A
ApaI  (8060)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (8056)
1 site
G G G C C C C C C G G G
HpaI  (7761)
1 site
G T T A A C C A A T T G
BspQI  (7455)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (7455)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
FspAI  (6984)
1 site
R T G C G C A Y Y A C G C G T R
EcoNI  (6834)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PfoI  (6694)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
SgrAI  (4746)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
XmnI  (0)
1 site
G A A N N N N T T C C T T N N N N A A G
BsaI  (148)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BssSI  (612)
1 site
C A C G A G G T G C T C
BssSαI  (612)
1 site
C A C G A G G T G C T C
BspEI  (1256)
1 site
T C C G G A A G G C C T
SnaBI  (1266)
1 site
T A C G T A A T G C A T
SfiI  (1886)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
EcoO109I  (2438)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BstZ17I  (3261)
1 site
G T A T A C C A T A T G
lacI
7536 .. 8618  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
7536 .. 8618  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RSF1010 RepB
4100 .. 5071  =  972 bp
323 amino acids  =  35.9 kDa
Product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepB
4100 .. 5071  =  972 bp
323 amino acids  =  35.9 kDa
Product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepC
6408 .. 7259  =  852 bp
283 amino acids  =  31.1 kDa
Product: replication protein C of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepC
6408 .. 7259  =  852 bp
283 amino acids  =  31.1 kDa
Product: replication protein C of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
CmR
1047 .. 1706  =  660 bp
219 amino acids  =  25.6 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
1047 .. 1706  =  660 bp
219 amino acids  =  25.6 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
RSF1010 oriV
2038 .. 2432  =  395 bp
replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)
RSF1010 oriV
2038 .. 2432  =  395 bp
replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)
cat promoter
944 .. 1046  =  103 bp
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
cat promoter
944 .. 1046  =  103 bp
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
RSF1010 oriT
2774 .. 2861  =  88 bp
origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 oriT
2774 .. 2861  =  88 bp
origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
rrnB T1 terminator
178 .. 264  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
178 .. 264  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
lacIq promoter
8619 .. 8696  =  78 bp
In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.
lacIq promoter
8619 .. 8696  =  78 bp
In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.
MCS
8995 .. 9051  =  57 bp
pUC18/19 multiple cloning site
MCS
8995 .. 9051  =  57 bp
pUC18/19 multiple cloning site
tac promoter
8926 .. 8954  =  29 bp
3 segments
   Segment 1:  -35  
   8926 .. 8931  =  6 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
8926 .. 8954  =  29 bp
3 segments
   Segment 2:  
   8932 .. 8947  =  16 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
8926 .. 8954  =  29 bp
3 segments
   Segment 3:  -10  
   8948 .. 8954  =  7 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
8926 .. 8954  =  29 bp
3 segments
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
rrnB T2 terminator
356 .. 383  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
rrnB T2 terminator
356 .. 383  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
lac operator
8962 .. 8978  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
8962 .. 8978  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RSF1010 RepA
5582 .. 6421  =  840 bp
279 amino acids  =  29.9 kDa
Product: replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepA
5582 .. 6421  =  840 bp
279 amino acids  =  29.9 kDa
Product: replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
ORF:  1624 .. 2031  =  408 bp
ORF:  135 amino acids  =  15.0 kDa
ORF:  494 .. 727  =  234 bp
ORF:  77 amino acids  =  8.9 kDa
ORF:  2942 .. 5071  =  2130 bp
ORF:  709 amino acids  =  78.0 kDa
ORF:  5582 .. 6421  =  840 bp
ORF:  279 amino acids  =  29.9 kDa
ORF:  1047 .. 1706  =  660 bp
ORF:  219 amino acids  =  25.6 kDa
ORF:  2238 .. 2666  =  429 bp
ORF:  142 amino acids  =  15.3 kDa
ORF:  2784 .. 3191  =  408 bp
ORF:  135 amino acids  =  14.3 kDa
ORF:  3690 .. 4103  =  414 bp
ORF:  137 amino acids  =  15.1 kDa
ORF:  4527 .. 5171  =  645 bp
ORF:  214 amino acids  =  22.8 kDa
ORF:  6309 .. 7259  =  951 bp
ORF:  316 amino acids  =  34.4 kDa
ORF:  8937 .. 97  =  237 bp
ORF:  78 amino acids  =  8.5 kDa
ORF:  425 .. 793  =  369 bp
ORF:  122 amino acids  =  14.1 kDa
ORF:  2459 .. 2743  =  285 bp
ORF:  94 amino acids  =  10.9 kDa
ORF:  2813 .. 3469  =  657 bp
ORF:  218 amino acids  =  22.9 kDa
ORF:  3662 .. 4000  =  339 bp
ORF:  112 amino acids  =  12.0 kDa
ORF:  4217 .. 4810  =  594 bp
ORF:  197 amino acids  =  20.5 kDa
ORF:  5579 .. 6346  =  768 bp
ORF:  255 amino acids  =  27.0 kDa
ORF:  6749 .. 7090  =  342 bp
ORF:  113 amino acids  =  12.6 kDa
ORF:  19 .. 345  =  327 bp
ORF:  108 amino acids  =  12.1 kDa
ORF:  1660 .. 1905  =  246 bp
ORF:  81 amino acids  =  9.4 kDa
ORF:  3109 .. 3339  =  231 bp
ORF:  76 amino acids  =  8.4 kDa
ORF:  4006 .. 4359  =  354 bp
ORF:  117 amino acids  =  12.6 kDa
ORF:  5833 .. 6198  =  366 bp
ORF:  121 amino acids  =  13.3 kDa
ORF:  7516 .. 7779  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  8368 .. 8685  =  318 bp
ORF:  105 amino acids  =  11.2 kDa
ORF:  1668 .. 1898  =  231 bp
ORF:  76 amino acids  =  8.5 kDa
ORF:  6642 .. 6980  =  339 bp
ORF:  112 amino acids  =  12.1 kDa
ORF:  7536 .. 8495  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
Click here to try SnapGene

Download pMMB207.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.