pYES1L

Linearized BAC/YAC shuttle vector for assembling large DNA fragments using the GeneArt® High-Order Genetic Assembly System.

Sequence Author: Thermo Fisher (Invitrogen)

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8000 6000 4000 2000 End (9380) NotI (9360) I-SceI (9322) AmpR promoter I-CeuI (59) PacI (16) Start (0) TRP1 TRP1 promoter CEN/ARS SmR ori2 repE incC sopA sopC oriT sopB pYES1L 9380 bp
End  (9380)
0 sites
NotI  (9360)
1 site
G C G G C C G C C G C C G G C G
I-SceI  (9322)
1 site
T A G G G A T A A C A G G G T A A T A T C C C T A T T G T C C C A T T A

I-SceI is a homing endonuclease that can recognize a variety of similar recognition sequences.
After cleavage, I-SceI can remain bound to DNA and alter its electrophoretic mobility.
I-CeuI  (59)
1 site
T A A C T A T A A C G G T C C T A A G G T A G C G A A T T G A T A T T G C C A G G A T T C C A T C G C T

I-CeuI is a homing endonuclease that can recognize a variety of similar recognition sequences.
PacI  (16)
1 site
T T A A T T A A A A T T A A T T
Start  (0)
0 sites
TRP1
172 .. 846  =  675 bp
224 amino acids  =  24.2 kDa
Product: phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis
yeast auxotrophic marker
TRP1
172 .. 846  =  675 bp
224 amino acids  =  24.2 kDa
Product: phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis
yeast auxotrophic marker
TRP1 promoter
847 .. 1127  =  281 bp
TRP1 promoter
847 .. 1127  =  281 bp
CEN/ARS
1386 .. 1889  =  504 bp
S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence
CEN/ARS
1386 .. 1889  =  504 bp
S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence
AmpR promoter
1926 .. 2030  =  105 bp
AmpR promoter
1926 .. 2030  =  105 bp
SmR
2514 .. 3305  =  792 bp
263 amino acids  =  29.2 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
SmR
2514 .. 3305  =  792 bp
263 amino acids  =  29.2 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
ori2
4367 .. 4586  =  220 bp
secondary origin of replication for the bacterial F plasmid; also known as oriS
ori2
4367 .. 4586  =  220 bp
secondary origin of replication for the bacterial F plasmid; also known as oriS
repE
4677 .. 5432  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the bacterial F plasmid
repE
4677 .. 5432  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the bacterial F plasmid
incC
5435 .. 5685  =  251 bp
incompatibility region of the bacterial F plasmid
incC
5435 .. 5685  =  251 bp
incompatibility region of the bacterial F plasmid
sopA
6011 .. 7186  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F plasmid
sopA
6011 .. 7186  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F plasmid
sopC
8230 .. 8703  =  474 bp
centromere-like partitioning region of the bacterial F plasmid
sopC
8230 .. 8703  =  474 bp
centromere-like partitioning region of the bacterial F plasmid
oriT
8994 .. 9284  =  291 bp
origin of transfer for the bacterial F plasmid (Frost et al., 1994)
oriT
8994 .. 9284  =  291 bp
origin of transfer for the bacterial F plasmid (Frost et al., 1994)
sopB
7186 .. 8157  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F plasmid
sopB
7186 .. 8157  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F plasmid
ORF:  3556 .. 3903  =  348 bp
ORF:  115 amino acids  =  12.9 kDa
ORF:  4666 .. 4896  =  231 bp
ORF:  76 amino acids  =  8.7 kDa
ORF:  4981 .. 5226  =  246 bp
ORF:  81 amino acids  =  8.9 kDa
ORF:  7186 .. 8157  =  972 bp
ORF:  323 amino acids  =  35.4 kDa
ORF:  74 .. 436  =  363 bp
ORF:  120 amino acids  =  13.3 kDa
ORF:  6011 .. 7186  =  1176 bp
ORF:  391 amino acids  =  44.1 kDa
ORF:  7538 .. 7936  =  399 bp
ORF:  132 amino acids  =  15.6 kDa
ORF:  2295 .. 3305  =  1011 bp
ORF:  336 amino acids  =  37.4 kDa
ORF:  4677 .. 5432  =  756 bp
ORF:  251 amino acids  =  29.4 kDa
ORF:  6192 .. 6344  =  153 bp
ORF:  50 amino acids  =  6.0 kDa
ORF:  315 .. 596  =  282 bp
ORF:  93 amino acids  =  10.8 kDa
ORF:  2553 .. 2936  =  384 bp
ORF:  127 amino acids  =  14.6 kDa
ORF:  4833 .. 5066  =  234 bp
ORF:  77 amino acids  =  9.1 kDa
ORF:  7227 .. 7463  =  237 bp
ORF:  78 amino acids  =  8.2 kDa
ORF:  2156 .. 2365  =  210 bp
ORF:  69 amino acids  =  8.1 kDa
ORF:  2546 .. 2803  =  258 bp
ORF:  85 amino acids  =  9.1 kDa
ORF:  2813 .. 3091  =  279 bp
ORF:  92 amino acids  =  9.4 kDa
ORF:  5912 .. 6064  =  153 bp
ORF:  50 amino acids  =  5.8 kDa
ORF:  6212 .. 6361  =  150 bp
ORF:  49 amino acids  =  5.8 kDa
ORF:  172 .. 846  =  675 bp
ORF:  224 amino acids  =  24.2 kDa
ORF:  1144 .. 1302  =  159 bp
ORF:  52 amino acids  =  5.8 kDa
ORF:  2059 .. 2256  =  198 bp
ORF:  65 amino acids  =  7.3 kDa
ORF:  3499 .. 3780  =  282 bp
ORF:  93 amino acids  =  10.7 kDa
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Individual Sequences & Maps

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