UnaG

Bilirubin-binding green fluorescent protein from the Japanese eel Anguilla japonica.
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No matches
400 300 200 100 End (420) BsaWI (401) FspI (394) AatII (383) ZraI (381) PshAI (328) BsaAI - SnaBI (297) BseRI (232) SmlI (194) BpuEI (179) TatI - BsrGI (122) BtgZI (107) BanII - Bsp1286I (78) BstXI (49) BanI (19) Start (0) UnaG UnaG 420 bp
End  (420)
0 sites
BsaWI  (401)
1 site
W C C G G W W G G C C W

Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
FspI  (394)
1 site
T G C G C A A C G C G T
AatII  (383)
1 site
G A C G T C C T G C A G
ZraI  (381)
1 site
G A C G T C C T G C A G
PshAI  (328)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BsaAI  (297)
1 site
Y A C G T R R T G C A Y
SnaBI  (297)
1 site
T A C G T A A T G C A T
BseRI  (232)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
SmlI  (194)
1 site
C T Y R A G G A R Y T C

Cleavage may be enhanced when more than one copy of the SmlI recognition sequence is present.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (179)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
TatI  (122)
1 site
W G T A C W W C A T G W
BsrGI  (122)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BtgZI  (107)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BanII  (78)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Bsp1286I  (78)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
BstXI  (49)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BanI  (19)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
Start  (0)
0 sites
UnaG
1 .. 420  =  420 bp
139 amino acids  =  15.6 kDa
Product: bilirubin-binding green fluorescent protein from Anguilla japonica (Kumagai et al., 2013)
UnaG
1 .. 420  =  420 bp
139 amino acids  =  15.6 kDa
Product: bilirubin-binding green fluorescent protein from Anguilla japonica (Kumagai et al., 2013)
ORF:  1 .. 420  =  420 bp
ORF:  139 amino acids  =  15.6 kDa
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Download UnaG.dna file

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Individual Sequences & Maps

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