pKatushka2S-N

Vector for fusing Katushka2S to the C-terminus of a partner protein.

Sequence Author: Evrogen

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HomePlasmidsFluorescent Protein Genes & PlasmidspKatushka2S-N
AflIII - PciI (4662) EcoO109I (3842) PfoI (3519) RsrII (3260) BsrDI (2977) PflFI - Tth111I (2862) FspI (2846) PluTI (2747) SfoI (2745) NarI (2744) KasI (2743) BspDI * - ClaI * (2584) StuI (2565) BseRI (2562) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) BglII (609) PaeR7I - XhoI (613) Eco53kI (618) SacI (620) HindIII (622) EcoRI (629) PstI (638) SalI (639) AccI (640) Acc65I (645) KpnI (649) SacII (652) PspOMI (653) TspMI - XmaI (656) ApaI (657) SmaI (658) BamHI (660) AgeI (666) AleI (679) BstXI (681) BclI * (701) BsrGI (728) BbsI (986) BsgI (1183) PshAI (1318) BlpI (1383) NotI (1388) XbaI * (1398) MfeI (1494) HpaI (1507) AflII (1626) DraIII (1860) CsiI - SexAI * (2333) pKatushka2S-N 4720 bp
AflIII  (4662)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (4662)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
EcoO109I  (3842)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
PfoI  (3519)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
RsrII  (3260)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2977)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2862)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2862)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2846)
1 site		 T G C G C A A C G C G T
PluTI  (2747)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2745)
1 site		 G G C G C C C C G C G G
NarI  (2744)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2743)
1 site		 G G C G C C C C G C G G
BspDI  (2584)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2584)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2565)
1 site		 A G G C C T T C C G G A
BseRI  (2562)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
AseI  (7)
1 site		 A T T A A T T A A T T A
NdeI  (234)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site		 T A C G T A A T G C A T
NheI  (591)
1 site		 G C T A G C C G A T C G
BmtI  (595)
1 site		 G C T A G C C G A T C G
AfeI  (596)
1 site		 A G C G C T T C G C G A
BglII  (609)
1 site		 A G A T C T T C T A G A
PaeR7I  (613)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (613)
1 site		 C T C G A G G A G C T C
Eco53kI  (618)
1 site		 G A G C T C C T C G A G
SacI  (620)
1 site		 G A G C T C C T C G A G
HindIII  (622)
1 site		 A A G C T T T T C G A A
EcoRI  (629)
1 site		 G A A T T C C T T A A G
PstI  (638)
1 site		 C T G C A G G A C G T C
SalI  (639)
1 site		 G T C G A C C A G C T G
AccI  (640)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (645)
1 site		 G G T A C C C C A T G G
KpnI  (649)
1 site		 G G T A C C C C A T G G
SacII  (652)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
PspOMI  (653)
1 site		 G G G C C C C C C G G G
TspMI  (656)
1 site		 C C C G G G G G G C C C
XmaI  (656)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
ApaI  (657)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
SmaI  (658)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BamHI  (660)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AgeI  (666)
1 site		 A C C G G T T G G C C A
AleI  (679)
1 site		 C A C N N N N G T G G T G N N N N C A C
BstXI  (681)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
BclI  (701)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BsrGI  (728)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
BbsI  (986)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsgI  (1183)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PshAI  (1318)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BlpI  (1383)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
NotI  (1388)
1 site		 G C G G C C G C C G C C G G C G
XbaI  (1398)
1 site		 T C T A G A A G A T C T
* Blocked by Dam methylation.
MfeI  (1494)
1 site		 C A A T T G G T T A A C
HpaI  (1507)
1 site		 G T T A A C C A A T T G
AflII  (1626)
1 site		 C T T A A G G A A T T C
DraIII  (1860)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
CsiI  (2333)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (2333)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
NeoR/KanR
2616 .. 3410  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
2616 .. 3410  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
Katushka2S
679 .. 1386  =  708 bp
235 amino acids  =  26.4 kDa
Product: improved version of the far-red fluorescent protein TurboFP635/Katushka (Luker et al., 2015)
mammalian codon-optimized
Katushka2S
679 .. 1386  =  708 bp
235 amino acids  =  26.4 kDa
Product: improved version of the far-red fluorescent protein TurboFP635/Katushka (Luker et al., 2015)
mammalian codon-optimized
ori
4018 .. 4606  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4018 .. 4606  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1636 .. 2091  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1636 .. 2091  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 promoter
2224 .. 2581  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
2224 .. 2581  =  358 bp
SV40 enhancer and early promoter
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
1508 .. 1629  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1508 .. 1629  =  122 bp
SV40 polyadenylation signal
AmpR promoter
2118 .. 2222  =  105 bp
AmpR promoter
2118 .. 2222  =  105 bp
MCS
591 .. 671  =  81 bp
multiple cloning site
MCS
591 .. 671  =  81 bp
multiple cloning site
HSV TK poly(A) signal
3642 .. 3689  =  48 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
3642 .. 3689  =  48 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
SV40 ori
2432 .. 2567  =  136 bp
SV40 origin of replication
SV40 ori
2432 .. 2567  =  136 bp
SV40 origin of replication
ORF:  679 .. 1386  =  708 bp
ORF:  235 amino acids  =  26.4 kDa
ORF:  2788 .. 3174  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  3431 .. 3880  =  450 bp
ORF:  149 amino acids  =  16.3 kDa
ORF:  2616 .. 3410  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  607 .. 1161  =  555 bp
ORF:  184 amino acids  =  19.3 kDa
ORF:  447 .. 734  =  288 bp
ORF:  95 amino acids  =  10.2 kDa
ORF:  792 .. 1037  =  246 bp
ORF:  81 amino acids  =  9.2 kDa
ORF:  2925 .. 3461  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  3636 .. 3869  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
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