pDendra2

Vector for expressing Dendra2 in bacteria.

Sequence Author: Clontech (TaKaRa)

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EcoO109I (4107) AatII (4053) ZraI (4051) XmnI (3730) PvuI (3501) FspI (3353) NmeAIII (3279) BsrFI (3211) BsaI (3192) AhdI (3131) AlwNI (2654) AflIII - PciI (2238) BspQI - SapI (2122) BstAPI (2062) PaeR7I - XhoI (1) PsiI (49) MfeI (59) lac operator ATG BseRI (115) BsaBI * (125) BamHI (126) TspMI - XmaI (140) SmaI (142) BclI * (154) EcoNI (156) KasI (285) NarI (286) SfoI (287) PluTI (289) AleI (324) BanII (568) PmlI (587) BstXI (689) BsrGI (838) HindIII (849) BlpI (861) NheI (969) BmtI (973) Bpu10I (994) BspEI (1217) PasI (1453) MscI (1488) NcoI - StyI (1522) XbaI (1825) PfoI (1882) PflFI - Tth111I (1985) AccI (2010) BstZ17I (2011) NdeI (2061) pDendra2 4123 bp
EcoO109I  (4107)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (4053)
1 site
G A C G T C C T G C A G
ZraI  (4051)
1 site
G A C G T C C T G C A G
XmnI  (3730)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (3501)
1 site
C G A T C G G C T A G C
FspI  (3353)
1 site
T G C G C A A C G C G T
NmeAIII  (3279)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrFI  (3211)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsaI  (3192)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3131)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2654)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AflIII  (2238)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2238)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2122)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2122)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstAPI  (2062)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T G G T T A A C
BseRI  (115)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BsaBI  (125)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BamHI  (126)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
TspMI  (140)
1 site
C C C G G G G G G C C C
XmaI  (140)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (142)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BclI  (154)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
EcoNI  (156)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
KasI  (285)
1 site
G G C G C C C C G C G G
NarI  (286)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (287)
1 site
G G C G C C C C G C G G
PluTI  (289)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
AleI  (324)
1 site
C A C N N N N G T G G T G N N N N C A C
BanII  (568)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
PmlI  (587)
1 site
C A C G T G G T G C A C
BstXI  (689)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BsrGI  (838)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
HindIII  (849)
1 site
A A G C T T T T C G A A
BlpI  (861)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
NheI  (969)
1 site
G C T A G C C G A T C G
BmtI  (973)
1 site
G C T A G C C G A T C G
Bpu10I  (994)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BspEI  (1217)
1 site
T C C G G A A G G C C T
PasI  (1453)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
MscI  (1488)
1 site
T G G C C A A C C G G T
NcoI  (1522)
1 site
C C A T G G G G T A C C
StyI  (1522)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
XbaI  (1825)
1 site
T C T A G A A G A T C T
PfoI  (1882)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
PflFI  (1985)
1 site
G A C N N N G T C C T<