pDsRed-Monomer-N1

Vector for fusing DsRed-Monomer to the C-terminus of a partner protein.

Sequence Author: Clontech (TaKaRa)

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AflIII - PciI (4633) ApaLI (4319) EcoO109I (3813) BsaI (3704) PfoI (3490) RsrII (3231) BsrDI (2948) PflFI - Tth111I (2833) MscI (2797) PluTI (2718) SfoI (2716) NarI (2715) KasI (2714) BspDI * - ClaI * (2555) SfiI (2490) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) PaeR7I - XhoI (613) Eco53kI (618) SacI (620) HindIII (622) EcoRI (629) SalI (639) AccI (640) Acc65I (645) KpnI (649) SacII (652) PspOMI (653) TspMI - XmaI (656) ApaI (657) SmaI (658) BamHI (660) AgeI (666) FspAI (727) BstEII (819) AhdI (858) SbfI (1019) BbsI (1099) BsrGI (1217) PflMI (1260) AleI (1306) NotI (1359) XbaI * (1369) MfeI (1465) HpaI (1478) BtsI - BtsαI (1554) AflII (1597) CsiI - SexAI * (2304) pDsRed-Monomer-N1 4691 bp
AflIII  (4633)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4633)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4319)
1 site
G T G C A C C A C G T G
EcoO109I  (3813)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3704)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3490)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3231)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2948)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2833)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2833)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
MscI  (2797)
1 site
T G G C C A A C C G G T
PluTI  (2718)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2716)
1 site
G G C G C C C C G C G G
NarI  (2715)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2714)
1 site
G G C G C C C C G C G G
BspDI  (2555)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2555)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
SfiI  (2490)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
PaeR7I  (613)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (613)
1 site
C T C G A G G A G C T C
Eco53kI  (618)
1 site
G A G C T C C T C G A G
SacI  (620)
1 site
G A G C T C C T C G A G
HindIII  (622)
1 site
A A G C T T T T C G A A
EcoRI  (629)
1 site
G A A T T C C T T A A G
SalI  (639)
1 site
G T C G A C C A G C T G
AccI  (640)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (645)
1 site
G G T A C C C C A T G G
KpnI  (649)
1 site
G G T A C C C C A T G G
SacII  (652)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PspOMI  (653)
1 site
G G G C C C C C C G G G
TspMI  (656)
1 site
C C C G G G G G G C C C
XmaI  (656)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
ApaI  (657)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SmaI  (658)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (660)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AgeI  (666)
1 site
A C C G G T T G G C C A
FspAI  (727)
1 site
R T G C G C A Y Y A C G C G T R
BstEII  (819)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AhdI  (858)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SbfI  (1019)
1 site
C C T G C A G G G G A C G T C C
BbsI  (1099)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsrGI  (1217)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
PflMI  (1260)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AleI  (1306)
1 site
C A C N N N N G T G G T G N N N N C A C
NotI  (1359)
1 site
G C G G C C G C C G C C G G C G
XbaI  (1369)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
MfeI  (1465)
1 site
C A A T T G G T T A A C
HpaI  (1478)
1 site
G T T A A C C A A T T G
BtsI  (1554)
1 site
G C A G T G N N C G T C A C
BtsαI  (1554)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
AflII  (1597)
1 site
C T T A A G G A A T T C
CsiI  (2304)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (2304)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
NeoR/KanR
2587 .. 3381  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
NeoR/KanR
2587 .. 3381  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
DsRed-Monomer
679 .. 1356  =  678 bp
225 amino acids  =  25.4 kDa
Product: monomeric derivative of DsRed fluorescent protein
mammalian codon-optimized
DsRed-Monomer
679 .. 1356  =  678 bp
225 amino acids  =  25.4 kDa
Product: monomeric derivative of DsRed fluorescent protein
mammalian codon-optimized
ori
3989 .. 4577  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3989 .. 4577  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1607 .. 2062  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis