pECFP-C1
Vector for fusing ECFP to the N-terminus of a partner protein
Sequence Author: Clontech (TaKaRa)
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Sticky ends from different AflIII sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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Sticky ends from different BsrDI sites may not be compatible. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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* Blocked by Dam methylation. |
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* Blocked by Dam methylation. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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ApaI can be used between 25°C and 37°C. |
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SmaI can be used at 37°C for brief incubations. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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* Blocked by Dam methylation. |
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* Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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Sticky ends from different DraIII sites may not be compatible. |
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Sticky ends from different CsiI sites may not be compatible. |
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* Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
NeoR/KanR 2627 .. 3421 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
NeoR/KanR 2627 .. 3421 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
ECFP 613 .. 1329 = 717 bp 239 amino acids = 26.9 kDa 3 segments Segment 1: 613 .. 615 = 3 bp 1 amino acid = 149.2 Da Product: enhanced CFP mammalian codon-optimized |
ECFP 613 .. 1329 = 717 bp 239 amino acids = 26.9 kDa 3 segments Segment 2: 1a 616 .. 618 = 3 bp 1 amino acid = 117.1 Da Product: enhanced CFP mammalian codon-optimized |
ECFP 613 .. 1329 = 717 bp 239 amino acids = 26.9 kDa 3 segments Segment 3: 619 .. 1329 = 711 bp 237 amino acids = 26.7 kDa Product: enhanced CFP mammalian codon-optimized |
ECFP 613 .. 1329 = 717 bp 239 amino acids = 26.9 kDa 3 segments Product: enhanced CFP mammalian codon-optimized |
ori 4029 .. 4617 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 4029 .. 4617 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
f1 ori 1647 .. 2102 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
f1 ori 1647 .. 2102 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
SV40 promoter 2235 .. 2592 = 358 bp SV40 enhancer and early promoter |
SV40 promoter 2235 .. 2592 = 358 bp SV40 enhancer and early promoter |
CMV enhancer 61 .. 364 = 304 bp human cytomegalovirus immediate early enhancer |
CMV enhancer 61 .. 364 = 304 bp human cytomegalovirus immediate early enhancer |
CMV promoter 365 .. 568 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
CMV promoter 365 .. 568 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
SV40 poly(A) signal 1519 .. 1640 = 122 bp SV40 polyadenylation signal |
SV40 poly(A) signal 1519 .. 1640 = 122 bp SV40 polyadenylation signal |
AmpR promoter 2129 .. 2233 = 105 bp |
AmpR promoter 2129 .. 2233 = 105 bp |
MCS 1330 .. 1395 = 66 bp multiple cloning site of fluorescent protein plasmids |
MCS 1330 .. 1395 = 66 bp multiple cloning site of fluorescent protein plasmids |
HSV TK poly(A) signal 3653 .. 3700 = 48 bp herpesvirus thymidine kinase polyadenylation signal |
HSV TK poly(A) signal 3653 .. 3700 = 48 bp herpesvirus thymidine kinase polyadenylation signal |
stop codons 1404 .. 1414 = 11 bp stop codons in all three reading frames |
stop codons 1404 .. 1414 = 11 bp stop codons in all three reading frames |
SV40 ori 2443 .. 2578 = 136 bp SV40 origin of replication |
SV40 ori 2443 .. 2578 = 136 bp SV40 origin of replication |
ORF: 613 .. 1410 = 798 bp ORF: 265 amino acids = 29.3 kDa |
ORF: 3442 .. 3891 = 450 bp ORF: 149 amino acids = 16.3 kDa |
ORF: 2627 .. 3421 = 795 bp ORF: 264 amino acids = 29.0 kDa |
ORF: 2799 .. 3185 = 387 bp ORF: 128 amino acids = 14.6 kDa |
ORF: 598 .. 1428 = 831 bp ORF: 276 amino acids = 27.7 kDa |
ORF: 2936 .. 3472 = 537 bp ORF: 178 amino acids = 19.8 kDa |
ORF: 3647 .. 3880 = 234 bp ORF: 77 amino acids = 8.6 kDa |
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