pDsRed-Express-N1
Vector for fusing rapidly maturing DsRed-Express to the C-terminus of a partner protein.
Sequence Author: Clontech (TaKaRa)
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Sticky ends from different AflIII sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Sticky ends from different PfoI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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Sticky ends from different BsrDI sites may not be compatible. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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| * Blocked by Dam methylation. |
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| * Blocked by Dam methylation. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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ApaI can be used between 25°C and 37°C. |
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SmaI can be used at 37°C for brief incubations. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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| * Blocked by Dcm methylation. Sticky ends from different PflMI sites may not be compatible. |
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Sticky ends from different BstXI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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| * Blocked by Dam methylation. |
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Sticky ends from different BtsαI sites may not be compatible. |
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Sticky ends from different DraIII sites may not be compatible. |
| NeoR/KanR 2585 .. 3379 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
| NeoR/KanR 2585 .. 3379 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
| DsRed-Express 679 .. 1356 = 678 bp 225 amino acids = 25.7 kDa Product: rapidly maturing tetrameric variant of DsRed fluorescent protein mammalian codon-optimized |
| DsRed-Express 679 .. 1356 = 678 bp 225 amino acids = 25.7 kDa Product: rapidly maturing tetrameric variant of DsRed fluorescent protein mammalian codon-optimized |
| ori 3987 .. 4575 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 3987 .. 4575 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| f1 ori 1605 .. 2060 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| f1 ori 1605 .. 2060 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| SV40 promoter 2193 .. 2550 = 358 bp SV40 enhancer and early promoter |
| SV40 promoter 2193 .. 2550 = 358 bp SV40 enhancer and early promoter |
| CMV enhancer 61 .. 364 = 304 bp human cytomegalovirus immediate early enhancer |
| CMV enhancer 61 .. 364 = 304 bp human cytomegalovirus immediate early enhancer |
| CMV promoter 365 .. 568 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| CMV promoter 365 .. 568 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
| SV40 poly(A) signal 1477 .. 1598 = 122 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 1477 .. 1598 = 122 bp SV40 polyadenylation signal |
| AmpR promoter 2087 .. 2191 = 105 bp |