pmOrange2-N1
Vector for fusing mOrange2 to the C-terminus of a partner protein.
Sequence Author: Clontech (TaKaRa)
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Sticky ends from different AflIII sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Sticky ends from different PfoI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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Sticky ends from different BsrDI sites may not be compatible. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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* Blocked by Dam methylation. |
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* Blocked by Dam methylation. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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ApaI can be used between 25°C and 37°C. |
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SmaI can be used at 37°C for brief incubations. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BlpI sites may not be compatible. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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Sticky ends from different PflMI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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The 1-base overhangs produced by XcmI may be hard to ligate.Sticky ends from different XcmI sites may not be compatible. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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* Blocked by Dam methylation. |
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Sticky ends from different BtsαI sites may not be compatible. |
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Sticky ends from different DraIII sites may not be compatible. |
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Sticky ends from different CsiI sites may not be compatible. |
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* Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
NeoR/KanR 2613 .. 3407 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
NeoR/KanR 2613 .. 3407 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
mOrange2 673 .. 1383 = 711 bp 236 amino acids = 26.8 kDa Product: photostable monomeric orange derivative of DsRed fluorescent protein mammalian codon-optimized |
mOrange2 673 .. 1383 = 711 bp 236 amino acids = 26.8 kDa Product: photostable monomeric orange derivative of DsRed fluorescent protein mammalian codon-optimized |
ori 4015 .. 4603 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 4015 .. 4603 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
f1 ori 1633 .. 2088 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
f1 ori 1633 .. 2088 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
SV40 promoter 2221 .. 2578 = 358 bp SV40 enhancer and early promoter |
SV40 promoter 2221 .. 2578 = 358 bp SV40 enhancer and early promoter |
CMV enhancer 61 .. 364 = 304 bp human cytomegalovirus immediate early enhancer |
CMV enhancer 61 .. 364 = 304 bp human cytomegalovirus immediate early enhancer |
CMV promoter 365 .. 568 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
CMV promoter 365 .. 568 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
SV40 poly(A) signal 1505 .. 1626 = 122 bp SV40 polyadenylation signal |
SV40 poly(A) signal 1505 .. 1626 = 122 bp SV40 polyadenylation signal |
AmpR promoter 2115 .. 2219 = 105 bp |
AmpR promoter 2115 .. 2219 = 105 bp |
MCS 591 .. 671 = 81 bp multiple cloning site of fluorescent protein plasmids |
MCS 591 .. 671 = 81 bp multiple cloning site of fluorescent protein plasmids |
HSV TK poly(A) signal 3639 .. 3686 = 48 bp herpesvirus thymidine kinase polyadenylation signal |
HSV TK poly(A) signal 3639 .. 3686 = 48 bp herpesvirus thymidine kinase polyadenylation signal |
SV40 ori 2429 .. 2564 = 136 bp SV40 origin of replication |
SV40 ori 2429 .. 2564 = 136 bp SV40 origin of replication |
ORF: 673 .. 1383 = 711 bp ORF: 236 amino acids = 26.8 kDa |
ORF: 2785 .. 3171 = 387 bp ORF: 128 amino acids = 14.6 kDa |
ORF: 3428 .. 3877 = 450 bp ORF: 149 amino acids = 16.3 kDa |
ORF: 2613 .. 3407 = 795 bp ORF: 264 amino acids = 29.0 kDa |
ORF: 607 .. 879 = 273 bp ORF: 90 amino acids = 9.7 kDa |
ORF: 447 .. 740 = 294 bp ORF: 97 amino acids = 10.6 kDa |
ORF: 2922 .. 3458 = 537 bp ORF: 178 amino acids = 19.8 kDa |
ORF: 3633 .. 3866 = 234 bp ORF: 77 amino acids = 8.6 kDa |
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