mAmetrine1.1

Yellow fluorescent protein with a large Stokes shift, excitable with violet light. Derived from EGFP. More photostable than the original mAmetrine.
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No matches
600 400 200 End (720) BsrGI (710) Bpu10I (604) BsiHKAI (509) BsiEI (464) EagI (461) NmeAIII (356) PpuMI - EcoO109I (323) SfcI (318) PfoI * (281) EciI (249) BtsI - BtsαI (214) DrdI (192) BssSI BssSαI (181) BtgZI (122) BanI (36) BseRI (31) Start (0) mAmetrine1.1 mAmetrine1.1 720 bp
End  (720)
0 sites
BsrGI  (710)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
Bpu10I  (604)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsiHKAI  (509)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
BsiEI  (464)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
EagI  (461)
1 site
C G G C C G G C C G G C
NmeAIII  (356)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PpuMI  (323)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
EcoO109I  (323)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
SfcI  (318)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
PfoI  (281)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
EciI  (249)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
BtsI  (214)
1 site
G C A G T G N N C G T C A C
BtsαI  (214)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
DrdI  (192)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BssSI  (181)
1 site
C A C G A G G T G C T C
BssSαI  (181)
1 site
C A C G A G G T G C T C
BtgZI  (122)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BanI  (36)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
Start  (0)
0 sites
mAmetrine1.1
1 .. 720  =  720 bp
239 amino acids  =  26.8 kDa
3 segments
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: photostable yellow fluorescent protein with a large Stokes shift, excitable with violet light (Ai et al., 2008)
mAmetrine1.1
1 .. 720  =  720 bp
239 amino acids  =  26.8 kDa
3 segments
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.1 Da
Product: photostable yellow fluorescent protein with a large Stokes shift, excitable with violet light (Ai et al., 2008)
mAmetrine1.1
1 .. 720  =  720 bp
239 amino acids  =  26.8 kDa
3 segments
   Segment 3:  
   7 .. 720  =  714 bp
   237 amino acids  =  26.6 kDa
Product: photostable yellow fluorescent protein with a large Stokes shift, excitable with violet light (Ai et al., 2008)
mAmetrine1.1
1 .. 720  =  720 bp
239 amino acids  =  26.8 kDa
3 segments
Product: photostable yellow fluorescent protein with a large Stokes shift, excitable with violet light (Ai et al., 2008)
ORF:  1 .. 720  =  720 bp
ORF:  239 amino acids  =  26.8 kDa
ORF:  3 .. 593  =  591 bp
ORF:  196 amino acids  =  22.7 kDa  (no start codon)
ORF:  1 .. 720  =  720 bp
ORF:  240 amino acids  =  24.6 kDa  (no start codon)
ORF:  318 .. 566  =  249 bp
ORF:  82 amino acids  =  9.1 kDa
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Download mAmetrine1.1.dna file

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