pAcGFP1-C1

Vector for fusing AcGFP1 to the N-terminus of a partner protein. For other reading frames, use pAcGFP1-C2 or pAcGFP1-C3.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsFluorescent Protein Genes & PlasmidspAcGFP1-C1
PciI (4673) ApaLI (4359) BsaI (3744) RsrII (3271) BsrDI (2988) PflFI - Tth111I (2873) FspI (2857) EagI (2661) BspDI * - ClaI * (2595) StuI (2576) BseRI (2573) SfiI (2530) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) BstEII (795) Bpu10I (805) BssHII (939) EcoNI (1237) PpuMI (1242) PflMI (1311) BspEI (1330) BglII (1339) PaeR7I - XhoI (1343) Eco53kI (1348) SacI (1350) HindIII (1352) EcoRI (1359) PstI (1368) SalI (1369) AccI (1370) Acc65I (1375) KpnI (1379) SacII (1382) PspOMI (1383) TspMI - XmaI (1386) ApaI (1387) SmaI (1388) BamHI (1390) XbaI * (1402) stop codons BclI * (1412) MfeI (1505) HpaI (1518) MluI (1641) CsiI - SexAI * (2344) pAcGFP1-C1 4731 bp
PciI  (4673)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
ApaLI  (4359)
1 site		 G T G C A C C A C G T G
BsaI  (3744)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
RsrII  (3271)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2988)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2873)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2873)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2857)
1 site		 T G C G C A A C G C G T
EagI  (2661)
1 site		 C G G C C G G C C G G C
BspDI  (2595)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2595)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2576)
1 site		 A G G C C T T C C G G A
BseRI  (2573)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
SfiI  (2530)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site		 A T T A A T T A A T T A
NdeI  (234)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site		 T A C G T A A T G C A T
NheI  (591)
1 site		 G C T A G C C G A T C G
BmtI  (595)
1 site		 G C T A G C C G A T C G
AfeI  (596)
1 site		 A G C G C T T C G C G A
AgeI  (600)
1 site		 A C C G G T T G G C C A
BstEII  (795)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
Bpu10I  (805)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BssHII  (939)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
EcoNI  (1237)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PpuMI  (1242)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
PflMI  (1311)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
BspEI  (1330)
1 site		 T C C G G A A G G C C T
BglII  (1339)
1 site		 A G A T C T T C T A G A
PaeR7I  (1343)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1343)
1 site		 C T C G A G G A G C T C
Eco53kI  (1348)
1 site		 G A G C T C C T C G A G
SacI  (1350)
1 site		 G A G C T C C T C G A G
HindIII  (1352)
1 site		 A A G C T T T T C G A A
EcoRI  (1359)
1 site		 G A A T T C C T T A A G
PstI  (1368)
1 site		 C T G C A G G A C G T C
SalI  (1369)
1 site		 G T C G A C C A G C T G
AccI  (1370)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (1375)
1 site		 G G T A C C C C A T G G
KpnI  (1379)
1 site		 G G T A C C C C A T G G
SacII  (1382)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
PspOMI  (1383)
1 site		 G G G C C C C C C G G G
TspMI  (1386)
1 site		 C C C G G G G G G C C C
XmaI  (1386)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
ApaI  (1387)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
SmaI  (1388)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BamHI  (1390)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (1402)
1 site		 T C T A G A A G A T C T
* Blocked by Dam methylation.
BclI  (1412)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
MfeI  (1505)
1 site		 C A A T T G G T T A A C
HpaI  (1518)
1 site		 G T T A A C C A A T T G
MluI  (1641)
1 site		 A C G C G T T G C G C A
CsiI  (2344)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (2344)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
NeoR/KanR
2627 .. 3421  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
NeoR/KanR
2627 .. 3421  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
AcGFP1
613 .. 1329  =  717 bp
239 amino acids  =  26.9 kDa
Product: Aequorea coerulescens GFP
mammalian codon-optimized
AcGFP1
613 .. 1329  =  717 bp
239 amino acids  =  26.9 kDa
Product: Aequorea coerulescens GFP
mammalian codon-optimized
ori
4029 .. 4617  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4029 .. 4617  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1647 .. 2102  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1647 .. 2102  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 promoter
2235 .. 2592  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
2235 .. 2592  =  358 bp
SV40 enhancer and early promoter
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
1519 .. 1640  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1519 .. 1640  =  122 bp
SV40 polyadenylation signal
AmpR promoter
2129 .. 2233  =  105 bp
AmpR promoter
2129 .. 2233  =  105 bp
MCS
1330 .. 1395  =  66 bp
multiple cloning site of fluorescent protein plasmids
MCS
1330 .. 1395  =  66 bp
multiple cloning site of fluorescent protein plasmids
HSV TK poly(A) signal
3653 .. 3700  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3653 .. 3700  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
stop codons
1404 .. 1414  =  11 bp
stop codons in all three reading frames
stop codons
1404 .. 1414  =  11 bp
stop codons in all three reading frames
SV40 ori
2443 .. 2578  =  136 bp
SV40 origin of replication
SV40 ori
2443 .. 2578  =  136 bp
SV40 origin of replication
ORF:  613 .. 1410  =  798 bp
ORF:  265 amino acids  =  29.3 kDa
ORF:  3442 .. 3891  =  450 bp
ORF:  149 amino acids  =  16.3 kDa
ORF:  2627 .. 3421  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2799 .. 3185  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  829 .. 1428  =  600 bp
ORF:  199 amino acids  =  20.4 kDa
ORF:  2936 .. 3472  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  3647 .. 3880  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
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Individual Sequences & Maps

AcGFP1
mClover3
pDsRed-Express2-1
pPAmCherry1-C1
Azami-Green
mCyRFP1
pDsRed-Express2-C1
pPAmCherry1-N1
Azurite BFP
mECFP
pDsRed-Express2-N1
pPhi-Yellow-B
BFP
Medium-FT
pDsRed-Monomer-C In-Fusion Ready
pPhi-Yellow-C
CFP
mEGFP
pDsRed-Monomer-C1
pPhi-Yellow-N
Citrine
mEmerald
pDsRed-Monomer-N In-Fusion Ready
pPhi-Yellow-PRL
Clover
mEos2
pDsRed-Monomer-N1
pPS-CFP2-C
CopGFP
mEos3.2
pDsRed2
pPS-CFP2-N
cp173Venus
mEos4a
pDsRed2-1
pPSmOrange-C1
Cycle 3 GFP
mEos4b
pDsRed2-C1
pPSmOrange-N1
CyOFP1
mEYFP
pDsRed2-N1
pRSET-BFP
CyPet
mgfp5
pE2-Crimson
pRSET-CFP
CyPet (humanized)
mGold
pE2-Crimson-C1
pRSET-EmGFP
CyRFP1
mGreenLantern
pE2-Crimson-N1
PS-CFP2
d1EGFP
mHoneydew
pECFP
PSmOrange
d2ECFP
MiCy
pECFP-1
PSmOrange2
d2EGFP
mIFP
pECFP-C1
pTagBFP-C
d2EYFP
miniSOG
pECFP-N1
pTagBFP-N
d4EGFP
miRFP670
pEGFP
pTagCFP-C
daGFP
miRFP703
pEGFP-1
pTagCFP-N
Dendra2
miRFP709
pEGFP-C1
pTagGFP2-C
dKeima-Red
mKalama1
pEGFP-C2
pTagGFP2-N
dKeima570
mKate2
pEGFP-C3
pTagRFP-C
Dronpa-Green1
mKeima-Red
pEGFP-N1
pTagRFP-N
Dronpa-Green3
mKikGR1
pEGFP-N2
pTagYFP-C
DsRed-Express
mKO
pEGFP-N3
pTagYFP-N
DsRed-Express2
mKO2
pEYFP
ptd-Tomato-N1
DsRed-Max
mMaple
pEYFP-1
ptdTomato
DsRed-Monomer
mMaroon1
pEYFP-C1
ptdTomato-C1
DsRed.T3
mMiCy1
pEYFP-N1
pTimer
DsRed1
mNectarine
pFusionRed-B
pTimer-1
DsRed2
mNeonGreen
pFusionRed-C
pTurboFP602-B
dTomato
mNeptune
pFusionRed-N
pTurboFP602-C
E2-Crimson
mNeptune2
pGFP
pTurboFP602-N
E2-Orange
mNeptune2.5
pGFPuv
pTurboFP602-PRL
E2-Red_Green
mOrange
pGLO
pTurboGFP-B
EBFP
mOrange2
pHcRed1
pTurboGFP-C
EBFP2
moxGFP
pHcRed1-1
pTurboGFP-N
ECFP
mPapaya1
pHcRed1-C1
pTurboGFP-PRL
ecliptic pHluorin
mPlum
pHcRed1-N1_1
pTurboRFP-B
EGFP
mRaspberry
phdKeima-Red-S1
pTurboRFP-C
Emerald GFP
mRFP1
phdKeima570-S1
pTurboRFP-N
EosFP
mRuby
phKikGR1-S1
pTurboRFP-PRL
EYFP
mRuby2
phKO1-S1
pTurboYFP-B
FAPalpha2
mRuby3
phmAG1-S1
pTurboYFP-C
FAPbeta1
mScarlet
phMGFP
pTurboYFP-N
Fast-FT
mScarlet-2I
phmKeima-Red-S1
pTurboYFP-PRL
Fluorescent Timer
mScarlet-H
phmKO1-S1
pZsGreen
FusionRed
mScarlet-I
phmUkG1-S1
pZsGreen1-1
Gamillus
mseCFP
pHRed
pZsGreen1-C1
GFP
msGFP2
pHTomato
pZsGreen1-N1
GFPuv
mTagBFP2
pHuji
pZsYellow
HcRed1
mTangerine
pKaede-S1
pZsYellow1-C1
hdKeima-Red
mTFP1
pKatushka2S-B
pZsYellow1-N1
hdKeima570
mTurquoise
pKatushka2S-C
ratiometric pHluorin
hKikGR1
mTurquoise2
pKatushka2S-N
Rhacostoma GFP
hKO
mUkG1
pKikGR1-S1
rsEGFP
hmAzami-Green
mVenus
pKillerOrange-B
rsEGFP2
hMGFP
mWasabi
pKillerOrange-C
rsFusionRed1
hmKeima-Red
PA-GFP
pKillerOrange-N
rsFusionRed2
hmKeima8.5
PA-TagRFP
pKillerRed-B
rsFusionRed3
hmKikGR1
pAcGFP1
pKillerRed-C
rsTagRFP
hmKO
pAcGFP1-1
pKillerRed-N
SBFP2
hmKO2
pAcGFP1-C In-Fusion Ready
pKindling-Red-B
SCFP3A
hmMiCy1
pAcGFP1-C1
pKindling-Red-N
SGFP2
hmUkG1
pAcGFP1-C2
pKO1-S1
SiriusGFP
hrGFP
pAcGFP1-C3
pLSSmOrange-C1
Skylan-NS
IFP1.1
pAcGFP1-N In-Fusion Ready
pLSSmOrange-N1
Slow-FT
IFP1.4
pAcGFP1-N1
pmAG1-S1
smURFP
IFP2.0
pAcGFP1-N2
pmBanana
super-ecliptic pHluorin
iRFP670
pAcGFP1-N3
pmCherry
superfolder GFP
iRFP682
pAG-S1
pmCherry-1
SuperNova
iRFP702
PAmCherry
pmCherry-C1
SuperNova2