pMFAPbeta1

Vector expressing a plasma membrane-localized version of the fluorogen-activating protein FAPβ1.

Sequence Author: SpectraGenetics

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HomePlasmidsFluorescent Protein Genes & PlasmidspMFAPbeta1
PsiI (6085) DraIII (5960) XmnI (5407) ScaI (5288) PvuI (5178) AhdI (4808) SexAI * (4560) BspDI * - ClaI * (4309) BsaBI * (4292) PluTI (4152) SfoI (4150) NarI (4149) KasI (4148) PflFI - Tth111I (4032) BclI * (3808) RsrII (3638) BspQI - SapI (3600) BstBI (3473) PfoI (3377) BssHII - MauBI (1) CMV enhancer SnaBI (365) HindIII (664) BstXI (709) EcoRV (729) HA PspOMI (828) ApaI (832) SfiI (836) HA NheI (926) BmtI (930) EcoNI (1056) BlpI (1178) BglII (1190) AleI (1205) BstEII (1282) BsmBI - Esp3I (1294) TspMI - XmaI (1397) SmaI (1399) SfiI (1704) SacII (1718) SalI (1727) AccI (1728) BsmI (1768) XcmI (1846) NotI (1917) PaeR7I - XhoI (1923) AflIII - PciI (2235) pMFAP β1 6184 bp
PsiI  (6085)
1 site		 T T A T A A A A T A T T
DraIII  (5960)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
XmnI  (5407)
1 site		 G A A N N N N T T C C T T N N N N A A G
ScaI  (5288)
1 site		 A G T A C T T C A T G A
PvuI  (5178)
1 site		 C G A T C G G C T A G C
AhdI  (4808)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SexAI  (4560)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BspDI  (4309)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (4309)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
BsaBI  (4292)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
PluTI  (4152)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (4150)
1 site		 G G C G C C C C G C G G
NarI  (4149)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (4148)
1 site		 G G C G C C C C G C G G
PflFI  (4032)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4032)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BclI  (3808)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
RsrII  (3638)
1 site		 C G G