pTurboRFP-B

EcoO109I  (4086) 1 site

Sticky ends from different EcoO109I sites may not be compatible.

AatII  (4032) 1 site

ZraI  (4030) 1 site

XmnI  (3709) 1 site

PvuI  (3480) 1 site

FspI  (3332) 1 site

NmeAIII  (3258) 1 site

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be compatible. For full activity, add fresh S-adenosylmethionine (SAM).

BsrFI  (3190) 1 site

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present. After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.

AhdI  (3110) 1 site

The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible.

AlwNI  (2633) 1 site

Sticky ends from different AlwNI sites may not be compatible.

AflIII  (2217) 1 site

Sticky ends from different AflIII sites may not be compatible.

PciI  (2217) 1 site

PciI is inhibited by nonionic detergents.

BspQI  (2101) 1 site

Sticky ends from different BspQI sites may not be compatible.

SapI  (2101) 1 site

Sticky ends from different SapI sites may not be compatible. SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.

BstAPI  (2041) 1 site

Sticky ends from different BstAPI sites may not be compatible.

AvaI  (1) 1 site

Sticky ends from different AvaI sites may not be compatible.

BsoBI  (1) 1 site

Sticky ends from different BsoBI sites may not be compatible. BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.

PaeR7I  (1) 1 site

PaeR7I does not recognize the sequence CTCTCGAG.

XhoI  (1) 1 site

PsiI  (49) 1 site

MfeI  (59) 1 site

BseRI  (115) 1 site

Sticky ends from different BseRI sites may not be compatible. BseRI quickly loses activity at 37°C. Prolonged incubation with BseRI may lead to degradation of the DNA.

BsaBI  (125) 1 site

* Blocked by Dam methylation.

BamHI  (126) 1 site

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.

BclI  (142) 1 site

* Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C.

BsrGI  (169) 1 site

BsrGI is typically used at 37°C, but is even more active at 60°C.

Bsu36I  (385) 1 site

Sticky ends from different Bsu36I sites may not be compatible.

SfiI  (595) 1 site

Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible.

BsgI  (624) 1 site

Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible. For full activity, add fresh S-adenosylmethionine (SAM).

BfuAI  (646) 1 site

Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible. BfuAI is typically used at 50°C, but is 50% active at 37°C.

BspMI  (646) 1 site

Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible.

DraIII  (713) 1 site

Sticky ends from different DraIII sites may not be compatible.

PshAI  (759) 1 site

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.

HindIII  (827) 1 site

BlpI  (839) 1 site

Sticky ends from different BlpI sites may not be compatible.

NheI  (947) 1 site

BmtI  (951) 1 site

Bpu10I  (972) 1 site

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site. Sticky ends from different Bpu10I sites may not be compatible.

PvuII  (1099) 1 site

BspEI  (1195) 1 site

BtgI  (1500) 1 site

Sticky ends from different BtgI sites may not be compatible.

NcoI  (1500) 1 site

StyI  (1500) 1 site

Sticky ends from different StyI sites may not be compatible.

XbaI  (1804) 1 site

PfoI  (1861) 1 site

Sticky ends from different PfoI sites may not be compatible.

PflFI  (1964) 1 site

The 1-base overhangs produced by PflFI may be hard to ligate. Sticky ends from different PflFI sites may not be compatible.

Tth111I  (1964) 1 site

The 1-base overhangs produced by Tth111I may be hard to ligate. Sticky ends from different Tth111I sites may not be compatible.

BsaAI  (1971) 1 site

AccI  (1989) 1 site

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence. Sticky ends from different AccI sites may not be compatible.

BstZ17I  (1990) 1 site

NdeI  (2040) 1 site

Prolonged incubation with NdeI may lead to removal of additional nucleotides.

AmpR 3037 .. 3897  =  861 bp 286 amino acids  =  31.5 kDa 2 segments    Segment 2:      3037 .. 3828  =  792 bp    263 amino acids  =  28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 3037 .. 3897  =  861 bp 286 amino acids  =  31.5 kDa 2 segments    Segment 1:  signal sequence      3829 .. 3897  =  69 bp    23 amino acids  =  2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR 3037 .. 3897  =  861 bp 286 amino acids  =  31.5 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics

ATG 114 .. 116  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

ATG 114 .. 116  =  3 bp 1 amino acid  =  149.2 Da Product: start codon

TurboRFP 132 .. 827  =  696 bp 231 amino acids  =  26.1 kDa Product: red fluorescent protein from Entacmaea quadricolor mammalian codon-optimized

TurboRFP 132 .. 827  =  696 bp 231 amino acids  =  26.1 kDa Product: red fluorescent protein from Entacmaea quadricolor mammalian codon-optimized

CmR 986 .. 1645  =  660 bp 219 amino acids  =  25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol

CmR 986 .. 1645  =  660 bp 219 amino acids  =  25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol

ori 2278 .. 2866  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori 2278 .. 2866  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

AmpR promoter 3898 .. 4002  =  105 bp

AmpR promoter 3898 .. 4002  =  105 bp

lambda t0 terminator 848 .. 942  =  95 bp transcription terminator from phage lambda

lambda t0 terminator 848 .. 942  =  95 bp transcription terminator from phage lambda

rrnB T1 terminator 1710 .. 1796  =  87 bp transcription terminator T1 from the E. coli rrnB gene

rrnB T1 terminator 1710 .. 1796  =  87 bp transcription terminator T1 from the E. coli rrnB gene

T5 promoter 10 .. 54  =  45 bp 4 segments    Segment 1:      10 .. 24  =  15 bp bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator

T5 promoter 10 .. 54  =  45 bp 4 segments    Segment 2:  -35      25 .. 30  =  6 bp bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator

T5 promoter 10 .. 54  =  45 bp 4 segments    Segment 3:      31 .. 47  =  17 bp bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator

T5 promoter 10 .. 54  =  45 bp 4 segments    Segment 4:  -10      48 .. 54  =  7 bp bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator

T5 promoter 10 .. 54  =  45 bp 4 segments bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator

lac operator 62 .. 78  =  17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

lac operator 62 .. 78  =  17 bp The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

lac operator 30 .. 46  =  17 bp The lac repressor binds to the lac operator to inhib