pPAmCherry-C1
Vector for fusing photoactivatable mCherry to the N-terminus of a partner protein.
Sequence Author: Clontech (TaKaRa)
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Sticky ends from different PfoI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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Sticky ends from different BsrDI sites may not be compatible. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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* Blocked by Dam methylation. |
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* Blocked by Dam methylation. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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Sticky ends from different PflMI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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The 1-base overhangs produced by XcmI may be hard to ligate.Sticky ends from different XcmI sites may not be compatible. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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ApaI can be used between 25°C and 37°C. |
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SmaI can be used at 37°C for brief incubations. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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* Blocked by Dam methylation. |
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* Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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Sticky ends from different BtsαI sites may not be compatible. |
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Sticky ends from different DraIII sites may not be compatible. |
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Sticky ends from different CsiI sites may not be compatible. |
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* Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
NeoR/KanR 2617 .. 3411 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
NeoR/KanR 2617 .. 3411 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
PAmCherry 612 .. 1319 = 708 bp 236 amino acids = 26.8 kDa Product: photoactivatable monomeric derivative of DsRed fluorescent protein mammalian codon-optimized |
PAmCherry 612 .. 1319 = 708 bp 236 amino acids = 26.8 kDa Product: photoactivatable monomeric derivative of DsRed fluorescent protein mammalian codon-optimized |
ori 4019 .. 4607 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 4019 .. 4607 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
f1 ori 1637 .. 2092 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
f1 ori 1637 .. 2092 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
SV40 promoter 2225 .. 2582 = 358 bp SV40 enhancer and early promoter |
SV40 promoter 2225 .. 2582 = 358 bp SV40 enhancer and early promoter |
CMV enhancer 61 .. 364 = 304 bp human cytomegalovirus immediate early enhancer |
CMV enhancer 61 .. 364 = 304 bp human cytomegalovirus immediate early enhancer |
CMV promoter 365 .. 568 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
CMV promoter 365 .. 568 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
SV40 poly(A) signal 1509 .. 1630 = 122 bp SV40 polyadenylation signal |
SV40 poly(A) signal 1509 .. 1630 = 122 bp SV40 polyadenylation signal |
AmpR promoter 2119 .. 2223 = 105 bp |
AmpR promoter 2119 .. 2223 = 105 bp |
MCS 1320 .. 1385 = 66 bp multiple cloning site of fluorescent protein plasmids |
MCS 1320 .. 1385 = 66 bp multiple cloning site of fluorescent protein plasmids |
HSV TK poly(A) signal 3643 .. 3690 = 48 bp herpesvirus thymidine kinase polyadenylation signal |
HSV TK poly(A) signal 3643 .. 3690 = 48 bp herpesvirus thymidine kinase polyadenylation signal |
SV40 ori 2433 .. 2568 = 136 bp SV40 origin of replication |
SV40 ori 2433 .. 2568 = 136 bp SV40 origin of replication |
ORF: 2617 .. 3411 = 795 bp ORF: 264 amino acids = 29.0 kDa |
ORF: 2789 .. 3175 = 387 bp ORF: 128 amino acids = 14.6 kDa |
ORF: 612 .. 1400 = 789 bp ORF: 262 amino acids = 29.2 kDa |
ORF: 3432 .. 3881 = 450 bp ORF: 149 amino acids = 16.3 kDa |
ORF: 2926 .. 3462 = 537 bp ORF: 178 amino acids = 19.8 kDa |
ORF: 3637 .. 3870 = 234 bp ORF: 77 amino acids = 8.6 kDa |
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