pAsRed2-C1
Vector for fusing AsRed2 to the N-terminus of a partner protein.
Sequence Author: Clontech (TaKaRa)
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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Sticky ends from different BsrDI sites may not be compatible. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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* Blocked by Dam methylation. |
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* Blocked by Dam methylation. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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FseI gradually loses activity when stored at -20°C. |
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.Cleavage can be improved with PaqCI Activator. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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ApaI can be used between 25°C and 37°C. |
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SmaI can be used at 37°C for brief incubations. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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* Blocked by Dam methylation. |
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* Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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Sticky ends from different BtsαI sites may not be compatible. |
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Sticky ends from different DraIII sites may not be compatible. |
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Sticky ends from different CsiI sites may not be compatible. |
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* Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
NeoR/KanR 2606 .. 3400 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
NeoR/KanR 2606 .. 3400 = 795 bp 264 amino acids = 29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin) |
AsRed2 613 .. 1308 = 696 bp 232 amino acids = 25.8 kDa Product: Anemonia sulcata red fluorescent protein mammalian codon-optimized |
AsRed2 613 .. 1308 = 696 bp 232 amino acids = 25.8 kDa Product: Anemonia sulcata red fluorescent protein mammalian codon-optimized |
ori 4008 .. 4596 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 4008 .. 4596 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
f1 ori 1626 .. 2081 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
f1 ori 1626 .. 2081 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
SV40 promoter 2214 .. 2571 = 358 bp SV40 enhancer and early promoter |
SV40 promoter 2214 .. 2571 = 358 bp SV40 enhancer and early promoter |
CMV enhancer 61 .. 364 = 304 bp human cytomegalovirus immediate early enhancer |
CMV enhancer 61 .. 364 = 304 bp human cytomegalovirus immediate early enhancer |
CMV promoter 365 .. 568 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
CMV promoter 365 .. 568 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
SV40 poly(A) signal 1498 .. 1619 = 122 bp SV40 polyadenylation signal |
SV40 poly(A) signal 1498 .. 1619 = 122 bp SV40 polyadenylation signal |
AmpR promoter 2108 .. 2212 = 105 bp |
AmpR promoter 2108 .. 2212 = 105 bp |
MCS 1309 .. 1374 = 66 bp multiple cloning site of fluorescent protein plasmids |
MCS 1309 .. 1374 = 66 bp multiple cloning site of fluorescent protein plasmids |
HSV TK poly(A) signal 3632 .. 3679 = 48 bp herpesvirus thymidine kinase polyadenylation signal |
HSV TK poly(A) signal 3632 .. 3679 = 48 bp herpesvirus thymidine kinase polyadenylation signal |
stop codons 1383 .. 1393 = 11 bp stop codons in all three reading frames |
stop codons 1383 .. 1393 = 11 bp stop codons in all three reading frames |
SV40 ori 2422 .. 2557 = 136 bp SV40 origin of replication |
SV40 ori 2422 .. 2557 = 136 bp SV40 origin of replication |
ORF: 613 .. 1389 = 777 bp ORF: 258 amino acids = 28.2 kDa |
ORF: 3421 .. 3870 = 450 bp ORF: 149 amino acids = 16.3 kDa |
ORF: 2606 .. 3400 = 795 bp ORF: 264 amino acids = 29.0 kDa |
ORF: 2778 .. 3164 = 387 bp ORF: 128 amino acids = 14.6 kDa |
ORF: 598 .. 1407 = 810 bp ORF: 269 amino acids = 26.0 kDa |
ORF: 2915 .. 3451 = 537 bp ORF: 178 amino acids = 19.8 kDa |
ORF: 3626 .. 3859 = 234 bp ORF: 77 amino acids = 8.6 kDa |
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