pAmCyan1-N1

Vector for fusing AmCyan1 to the C-terminus of a partner protein.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsFluorescent Protein Genes & PlasmidspAmCyan1-N1
AflIII - PciI (4645) ApaLI (4331) BsaI (3716) PfoI (3502) RsrII (3243) BsrDI (2960) PflFI - Tth111I (2845) FspI (2829) PluTI (2730) SfoI (2728) NarI (2727) KasI (2726) BspDI * - ClaI * (2567) BseRI (2545) SfiI (2502) AseI (7) CMV enhancer NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) BglII (609) PaeR7I - XhoI (613) Eco53kI (618) SacI (620) HindIII (622) EcoRI (629) SalI (639) AccI (640) Acc65I (645) KpnI (649) SacII (652) PspOMI (653) TspMI - XmaI (656) ApaI (657) SmaI (658) BamHI (660) AgeI (666) BstEII (819) BbsI (1105) KflI - PpuMI (1113) BmgBI (1164) BstXI - PmlI (1263) EcoNI (1297) BsgI (1336) AleI (1351) NotI (1371) XbaI * (1381) MfeI (1477) HpaI (1490) BtsI - BtsαI (1566) AflII (1609) CsiI - SexAI * (2316) pAmCyan1-N1 4703 bp
AflIII  (4645)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (4645)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
ApaLI  (4331)
1 site		 G T G C A C C A C G T G
BsaI  (3716)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3502)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
RsrII  (3243)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2960)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2845)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2845)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2829)
1 site		 T G C G C A A C G C G T
PluTI  (2730)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2728)
1 site		 G G C G C C C C G C G G
NarI  (2727)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2726)
1 site		 G G C G C C C C G C G G
BspDI  (2567)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2567)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
BseRI  (2545)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
SfiI  (2502)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site		 A T T A A T T A A T T A
NdeI  (234)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site		 T A C G T A A T G C A T
NheI  (591)
1 site		 G C T A G C C G A T C G
BmtI  (595)
1 site		 G C T A G C C G A T C G
AfeI  (596)
1 site		 A G C G C T T C G C G A
BglII  (609)
1 site		 A G A T C T T C T A G A
PaeR7I  (613)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (613)
1 site		 C T C G A G G A G C T C
Eco53kI  (618)
1 site		 G A G C T C C T C G A G
SacI  (620)
1 site		 G A G C T C C T C G A G
HindIII  (622)
1 site		 A A G C T T T T C G A A
EcoRI  (629)
1 site		 G A A T T C C T T A A G
SalI  (639)
1 site		 G T C G A C C A G C T G
AccI  (640)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (645)
1 site		 G G T A C C C C A T G G
KpnI  (649)
1 site		 G G T A C C C C A T G G
SacII  (652)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
PspOMI  (653)
1 site		 G G G C C C C C C G G G
TspMI  (656)
1 site		 C C C G G G G G G C C C
XmaI  (656)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
ApaI  (657)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
SmaI  (658)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BamHI  (660)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AgeI  (666)
1 site		 A C C G G T T G G C C A
BstEII  (819)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BbsI  (1105)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
KflI  (1113)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
PpuMI  (1113)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
BmgBI  (1164)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BstXI  (1263)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
PmlI  (1263)
1 site		 C A C G T G G T G C A C
EcoNI  (1297)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BsgI  (1336)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AleI  (1351)
1 site		 C A C N N N N G T G G T G N N N N C A C
NotI  (1371)
1 site		 G C G G C C G C C G C C G G C G
XbaI  (1381)
1 site		 T C T A G A A G A T C T
* Blocked by Dam methylation.
MfeI  (1477)
1 site		 C A A T T G G T T A A C
HpaI  (1490)
1 site		 G T T A A C C A A T T G
BtsI  (1566)
1 site		 G C A G T G N N C G T C A C
BtsαI  (1566)
1 site		 G C A G T G N N C G T C A C
Sticky ends from different BtsαI sites may not be compatible.
AflII  (1609)
1 site		 C T T A A G G A A T T C
CsiI  (2316)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (2316)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
NeoR/KanR
2599 .. 3393  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
NeoR/KanR
2599 .. 3393  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
AmCyan1
679 .. 1368  =  690 bp
229 amino acids  =  25.3 kDa
Product: Anemonia majano cyan fluorescent protein
mammalian codon-optimized
AmCyan1
679 .. 1368  =  690 bp
229 amino acids  =  25.3 kDa
Product: Anemonia majano cyan fluorescent protein
mammalian codon-optimized
ori
4001 .. 4589  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4001 .. 4589  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1619 .. 2074  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1619 .. 2074  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 promoter
2207 .. 2564  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
2207 .. 2564  =  358 bp
SV40 enhancer and early promoter
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
1491 .. 1612  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1491 .. 1612  =  122 bp
SV40 polyadenylation signal
AmpR promoter
2101 .. 2205  =  105 bp
AmpR promoter
2101 .. 2205  =  105 bp
MCS
591 .. 671  =  81 bp
multiple cloning site of fluorescent protein plasmids
MCS
591 .. 671  =  81 bp
multiple cloning site of fluorescent protein plasmids
HSV TK poly(A) signal
3625 .. 3672  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3625 .. 3672  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
SV40 ori
2415 .. 2550  =  136 bp
SV40 origin of replication
SV40 ori
2415 .. 2550  =  136 bp
SV40 origin of replication
ORF:  679 .. 1368  =  690 bp
ORF:  229 amino acids  =  25.3 kDa
ORF:  2599 .. 3393  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2771 .. 3157  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  3414 .. 3863  =  450 bp
ORF:  149 amino acids  =  16.3 kDa
ORF:  447 .. 713  =  267 bp
ORF:  88 amino acids  =  9.4 kDa
ORF:  2908 .. 3444  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  3619 .. 3852  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
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Individual Sequences & Maps

AcGFP1
mCyRFP1
pDsRed-Express2
pPAmCherry1-C1
Azami-Green
mECFP
pDsRed-Express2-1
pPAmCherry1-N1
Azurite BFP
Medium-FT
pDsRed-Express2-C1
pPhi-Yellow-B
BFP
mEGFP
pDsRed-Express2-N1
pPhi-Yellow-C
CFP
mEmerald
pDsRed-Monomer-C In-Fusion Ready
pPhi-Yellow-N
Citrine
mEos2
pDsRed-Monomer-C1
pPhi-Yellow-PRL
Clover
mEos3.1
pDsRed-Monomer-N In-Fusion Ready
pPS-CFP2-C
CopGFP
mEos3.2
pDsRed-Monomer-N1
pPS-CFP2-N
cp173Venus
mEos4a
pDsRed2
pPSmOrange-C1
Cycle 3 GFP
mEos4b
pDsRed2-1
pPSmOrange-N1
CyOFP1
mEYFP
pDsRed2-C1
pRSET-BFP
CyPet
mgfp5
pDsRed2-N1
pRSET-CFP
CyPet (humanized)
mGold
pE2-Crimson
pRSET-EmGFP
CyRFP1
mGreenLantern
pE2-Crimson-C1
PS-CFP2
d1EGFP
mHoneydew
pE2-Crimson-N1
PSmOrange
d2ECFP
MiCy
pECFP
PSmOrange2
d2EGFP
mIFP
pECFP-1
pTagBFP-C
d2EYFP
miniSOG
pECFP-C1
pTagBFP-N
d4EGFP
miRFP670
pECFP-N1
pTagCFP-C
daGFP
miRFP703
pEGFP
pTagCFP-N
Dendra2
miRFP709
pEGFP-1
pTagGFP2-C
dKeima-Red
mKalama1
pEGFP-C1
pTagGFP2-N
dKeima570
mKate2
pEGFP-C2
pTagRFP-C
Dronpa-Green1
mKeima-Red
pEGFP-C3
pTagRFP-N
Dronpa-Green3
mKikGR1
pEGFP-N1
pTagYFP-C
DsRed-Express
mKO
pEGFP-N2
pTagYFP-N
DsRed-Express2
mKO2
pEGFP-N3
ptd-Tomato-N1
DsRed-Max
mMaple
pEYFP
ptdTomato
DsRed-Monomer
mMaroon1
pEYFP-1
ptdTomato-C1
DsRed.T3
mMiCy1
pEYFP-C1
pTimer
DsRed1
mNectarine
pEYFP-N1
pTimer-1
DsRed2
mNeonGreen
pFusionRed-B
pTurboFP602-B
dTomato
mNeptune
pFusionRed-C
pTurboFP602-C
E2-Crimson
mNeptune2
pFusionRed-N
pTurboFP602-N
E2-Orange
mNeptune2.5
pGFP
pTurboFP602-PRL
E2-Red_Green
mOrange
pGFPuv
pTurboGFP-B
EBFP