pMFAPalpha2

Vector expressing a plasma membrane-localized version of the fluorogen-activating protein FAPα2.

Sequence Author: SpectraGenetics

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PsiI (5950) DraIII (5825) XmnI (5272) ScaI (5153) PvuI (5043) AhdI (4673) SexAI * (4425) StuI (4195) BspDI * - ClaI * (4174) BsaBI * (4157) PluTI (4017) SfoI (4015) NarI (4014) KasI (4013) BclI * (3673) RsrII (3503) BspQI - SapI (3465) BstBI (3338) BssHII - MauBI (1) CMV enhancer SnaBI (365) HindIII (664) Acc65I (670) KpnI (674) BstXI (709) EcoRI (713) EcoRV (729) HA PspOMI (828) ApaI (832) SfiI (836) BlpI (1080) BmgBI (1123) BspEI (1214) SfiI (1569) SacII (1583) PstI (1590) SalI (1592) AccI (1593) BsmI (1633) NotI (1782) AvaI - BmeT110I - BsoBI - PaeR7I - XhoI (1788) BbsI (1994) AflIII - PciI (2100) pMFAP α2 6049 bp
PsiI  (5950)
1 site
T T A T A A A A T A T T
DraIII  (5825)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
XmnI  (5272)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (5153)
1 site
A G T A C T T C A T G A
PvuI  (5043)
1 site
C G A T C G G C T A G C
AhdI  (4673)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SexAI  (4425)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
StuI  (4195)
1 site
A G G C C T T C C G G A
BspDI  (4174)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (4174)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
BsaBI  (4157)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
PluTI  (4017)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (4015)
1 site
G G C G C C C C G C G G
NarI  (4014)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (4013)
1 site
G G C G C C C C G C G G
BclI  (3673)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
RsrII  (3503)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BspQI  (3465)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3465)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstBI  (3338)
1 site
T T C G A A A A G C T T
BssHII  (1)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
MauBI  (1)
1 site
C G C G C G C G G C G C G C G C
SnaBI  (365)
1 site
T A C G T A A T G C A T
HindIII  (664)
1 site
A A G C T T T T C G A A
Acc65I  (670)
1 site
G G T A C C C C A T G G