pMFAPalpha2
Vector expressing a plasma membrane-localized version of the fluorogen-activating protein FAPα2.
Sequence Author: SpectraGenetics
Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets
No matches
|
| ||
Sticky ends from different DraIII sites may not be compatible. |
|
|
|
| ||
The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
| ||
| * Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
|
| ||
| * Blocked by Dam methylation. |
| ||
| * Blocked by Dam methylation. |
| ||
| * Blocked by Dam methylation. |
| ||
Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
|
| ||
Efficient cleavage requires at least two copies of the NarI recognition sequence. |
|
| ||
| * Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
| ||
Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
| ||
Sticky ends from different BspQI sites may not be compatible. |
| ||
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
|
| ||
BssHII is typically used at 50°C, but is 75% active at 37°C. |
|
|
|
|
|