pDsRed-Express-C1

Vector for fusing rapidly maturing DsRed-Express to the N-terminus of a partner protein.

Sequence Author: Clontech (TaKaRa)

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PciI (4622) ApaLI (4308) BsaI (3693) PfoI (3479) RsrII (3220) BsrDI (2937) PflFI - Tth111I (2822) PluTI (2707) SfoI (2705) NarI (2704) KasI (2703) EagI (2610) BspDI * - ClaI * (2544) SfiI (2479) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) FspAI (661) AhdI (792) SbfI (953) BbsI (1033) PflMI * (1131) BstXI (1155) BsgI (1192) AleI (1240) BssHII (1257) BglII (1288) PaeR7I - XhoI (1292) Eco53kI (1297) SacI (1299) HindIII (1301) EcoRI (1308) SalI (1318) AccI (1319) Acc65I (1324) KpnI (1328) SacII (1331) PspOMI (1332) TspMI - XmaI (1335) ApaI (1336) SmaI (1337) BamHI (1339) XbaI * (1351) stop codons BclI * (1361) MfeI (1454) HpaI (1467) BtsI - BtsαI (1543) MluI (1590) DraIII (1820) pDsRed-Express-C1 4680 bp
PciI  (4622)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4308)
1 site
G T G C A C C A C G T G
BsaI  (3693)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3479)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3220)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2937)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2822)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2822)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PluTI  (2707)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2705)
1 site
G G C G C C C C G C G G
NarI  (2704)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2703)
1 site
G G C G C C C C G C G G
EagI  (2610)
1 site
C G G C C G G C C G G C
BspDI  (2544)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2544)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
SfiI  (2479)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
AgeI  (600)
1 site
A C C G G T T G G C C A
FspAI  (661)
1 site
R T G C G C A Y Y A C G C G T R
AhdI  (792)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SbfI  (953)
1 site
C C T G C A G G G G A C G T C C
BbsI  (1033)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PflMI  (1131)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
BstXI  (1155)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BsgI  (1192)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AleI  (1240)
1 site
C A C N N N N G T G G T G N N N N C A C
BssHII  (1257)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BglII  (1288)
1 site
A G A T C T T C T A G A
PaeR7I  (1292)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1292)
1 site
C T C G A G G A G C T C
Eco53kI  (1297)
1 site
G A G C T C C T C G A G
SacI  (1299)
1 site
G A G C T C C T C G A G
HindIII  (1301)
1 site
A A G C T T T T C G A A
EcoRI  (1308)
1 site
G A A T T C C T T A A G
SalI  (1318)
1 site
G T C G A C C A G C T G
AccI  (1319)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (1324)
1 site
G G T A C C C C A T G G
KpnI  (1328)
1 site
G G T A C C C C A T G G
SacII  (1331)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PspOMI  (1332)
1 site
G G G C C C C C C G G G
TspMI  (1335)
1 site
C C C G G G G G G C C C
XmaI  (1335)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
ApaI  (1336)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SmaI  (1337)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (1339)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (1351)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
BclI  (1361)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
MfeI  (1454)
1 site
C A A T T G G