pDsRed-Express-C1

Vector for fusing rapidly maturing DsRed-Express to the N-terminus of a partner protein.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsFluorescent Protein Genes & PlasmidspDsRed-Express-C1
PciI (4622) ApaLI (4308) BsaI (3693) PfoI (3479) RsrII (3220) BsrDI (2937) PflFI - Tth111I (2822) PluTI (2707) SfoI (2705) NarI (2704) KasI (2703) EagI (2610) BspDI * - ClaI * (2544) SfiI (2479) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) FspAI (661) AhdI (792) SbfI (953) BbsI (1033) PflMI * (1131) BstXI (1155) BsgI (1192) AleI (1240) BssHII (1257) BglII (1288) PaeR7I - XhoI (1292) Eco53kI (1297) SacI (1299) HindIII (1301) EcoRI (1308) SalI (1318) AccI (1319) Acc65I (1324) KpnI (1328) SacII (1331) PspOMI (1332) TspMI - XmaI (1335) ApaI (1336) SmaI (1337) BamHI (1339) XbaI * (1351) stop codons BclI * (1361) MfeI (1454) HpaI (1467) BtsI - BtsαI (1543) MluI (1590) DraIII (1820) pDsRed-Express-C1 4680 bp
PciI  (4622)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
ApaLI  (4308)
1 site		 G T G C A C C A C G T G
BsaI  (3693)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3479)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
RsrII  (3220)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2937)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2822)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2822)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PluTI  (2707)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2705)
1 site		 G G C G C C C C G C G G
NarI  (2704)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2703)
1 site		 G G C G C C C C G C G G
EagI  (2610)
1 site		 C G G C C G G C C G G C
BspDI  (2544)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2544)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
SfiI  (2479)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site		 A T T A A T T A A T T A
NdeI  (234)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site		 T A C G T A A T G C A T
NheI  (591)
1 site		 G C T A G C C G A T C G
BmtI  (595)
1 site		 G C T A G C C G A T C G
AfeI  (596)
1 site		 A G C G C T T C G C G A
AgeI  (600)
1 site		 A C C G G T T G G C C A
FspAI  (661)
1 site		 R T G C G C A Y Y A C G C G T R
AhdI  (792)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SbfI  (953)
1 site		 C C T G C A G G G G A C G T C C
BbsI  (1033)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PflMI  (1131)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
BstXI  (1155)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
BsgI  (1192)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AleI  (1240)
1 site		 C A C N N N N G T G G T G N N N N C A C
BssHII  (1257)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
BglII  (1288)
1 site		 A G A T C T T C T A G A
PaeR7I  (1292)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1292)
1 site		 C T C G A G G A G C T C
Eco53kI  (1297)
1 site		 G A G C T C C T C G A G
SacI  (1299)
1 site		 G A G C T C C T C G A G
HindIII  (1301)
1 site		 A A G C T T T T C G A A
EcoRI  (1308)
1 site		 G A A T T C C T T A A G
SalI  (1318)
1 site		 G T C G A C C A G C T G
AccI  (1319)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (1324)
1 site		 G G T A C C C C A T G G
KpnI  (1328)
1 site		 G G T A C C C C A T G G
SacII  (1331)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
PspOMI  (1332)
1 site		 G G G C C C C C C G G G
TspMI  (1335)
1 site		 C C C G G G G G G C C C
XmaI  (1335)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
ApaI  (1336)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
SmaI  (1337)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BamHI  (1339)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (1351)
1 site		 T C T A G A A G A T C T
* Blocked by Dam methylation.
BclI  (1361)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
MfeI  (1454)
1 site		 C A A T T G G T T A A C
HpaI  (1467)
1 site		 G T T A A C C A A T T G
BtsI  (1543)
1 site		 G C A G T G N N C G T C A C
BtsαI  (1543)
1 site		 G C A G T G N N C G T C A C
Sticky ends from different BtsαI sites may not be compatible.
MluI  (1590)
1 site		 A C G C G T T G C G C A
DraIII  (1820)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
NeoR/KanR
2576 .. 3370  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
NeoR/KanR
2576 .. 3370  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
DsRed-Express
613 .. 1287  =  675 bp
225 amino acids  =  25.7 kDa
Product: rapidly maturing tetrameric variant of DsRed fluorescent protein
mammalian codon-optimized
DsRed-Express
613 .. 1287  =  675 bp
225 amino acids  =  25.7 kDa
Product: rapidly maturing tetrameric variant of DsRed fluorescent protein
mammalian codon-optimized
ori
3978 .. 4566  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3978 .. 4566  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1596 .. 2051  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1596 .. 2051  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 promoter
2184 .. 2541  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
2184 .. 2541  =  358 bp
SV40 enhancer and early promoter
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
61 .. 364  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
365 .. 568  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
1468 .. 1589  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1468 .. 1589  =  122 bp
SV40 polyadenylation signal
AmpR promoter
2078 .. 2182  =  105 bp
AmpR promoter
2078 .. 2182  =  105 bp
MCS
1288 .. 1344  =  57 bp
multiple cloning site of fluorescent protein plasmids
MCS
1288 .. 1344  =  57 bp
multiple cloning site of fluorescent protein plasmids
HSV TK poly(A) signal
3602 .. 3649  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3602 .. 3649  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
stop codons
1353 .. 1363  =  11 bp
stop codons in all three reading frames
stop codons
1353 .. 1363  =  11 bp
stop codons in all three reading frames
SV40 ori
2392 .. 2527  =  136 bp
SV40 origin of replication
SV40 ori
2392 .. 2527  =  136 bp
SV40 origin of replication
ORF:  613 .. 1359  =  747 bp
ORF:  248 amino acids  =  27.9 kDa
ORF:  3391 .. 3840  =  450 bp
ORF:  149 amino acids  =  16.3 kDa
ORF:  2576 .. 3370  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2748 .. 3134  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  598 .. 1377  =  780 bp
ORF:  259 amino acids  =  26.0 kDa
ORF:  2885 .. 3421  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  3596 .. 3829  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
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Individual Sequences & Maps

AcGFP1
mCyRFP1
pDsRed-Express2
pPAmCherry1-C1
Azami-Green
mECFP
pDsRed-Express2-1
pPAmCherry1-N1
Azurite BFP
Medium-FT
pDsRed-Express2-C1
pPhi-Yellow-B
BFP
mEGFP
pDsRed-Express2-N1
pPhi-Yellow-C
CFP
mEmerald
pDsRed-Monomer-C In-Fusion Ready
pPhi-Yellow-N
Citrine
mEos2
pDsRed-Monomer-C1
pPhi-Yellow-PRL
Clover
mEos3.1
pDsRed-Monomer-N In-Fusion Ready
pPS-CFP2-C
CopGFP
mEos3.2
pDsRed-Monomer-N1
pPS-CFP2-N
cp173Venus