pBIND-ER(alpha)
Renilla luciferase vector encoding the Gal4 DNA-binding domain fused to an estrogen receptor ligand-binding domain.
Sequence Author: Promega
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PciI is inhibited by nonionic detergents. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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Sticky ends from different BstXI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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Efficient cleavage requires at least two copies of the NaeI recognition sequence. |
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Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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Sticky ends from different EcoO109I sites may not be compatible. |
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Sticky ends from different Bsu36I sites may not be compatible. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
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Sticky ends from different Esp3I sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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SmaI can be used at 37°C for brief incubations. |
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Sticky ends from different CsiI sites may not be compatible. |
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| * Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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Sticky ends from different DraIII sites may not be compatible. |
| hRluc 3399 .. 4331 = 933 bp 311 amino acids = 36.0 kDa Product: Renilla luciferase human codon-optimized |
| hRluc 3399 .. 4331 = 933 bp 311 amino acids = 36.0 kDa Product: Renilla luciferase human codon-optimized |
| NeoR/KanR 4383 .. 5174 = 792 bp 263 amino acids = 28.9 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin®) |
| NeoR/KanR 4383 .. 5174 = 792 bp 263 amino acids = 28.9 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin®) |
| GAL4 DNA binding domain 1083 .. 1520 = 438 bp 146 amino acids = 16.8 kDa Product: DNA binding domain of the GAL4 transcriptional activator |
| GAL4 DNA binding domain 1083 .. 1520 = 438 bp 146 amino acids = 16.8 kDa Product: DNA binding domain of the GAL4 transcriptional activator |
| IgA linker 1527 .. 1553 = 27 bp 9 amino acids = 880.0 Da Product: peptide derived from a human Ig alpha chain |
| IgA linker 1527 .. 1553 = 27 bp 9 amino acids = 880.0 Da Product: peptide derived from a human Ig alpha chain |
| ER-LBD 1563 .. 2456 = 894 bp 297 amino acids = 33.5 kDa Product: estrogen nuclear receptor alpha ligand-binding domain |
| ER-LBD 1563 .. 2456 = 894 bp 297 amino acids = 33.5 kDa Product: estrogen nuclear receptor alpha ligand-binding domain |
| AmpR 5547 .. 6407 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 5547 .. 5615 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 5547 .. 6407 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 5616 .. 6407 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 5547 .. 6407 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| ori 6565 .. 7153 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 6565 .. 7153 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| SV40 promoter 2991 .. 3348 = 358 bp SV40 enhancer and early promoter |