pSF-CMV-FMDV-FLuc

Mammalian vector for co-expressing a gene together with firefly luciferase expressed from the foot-and-mouth disease virus IRES.

Sequence Author: Oxford Genetics

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PmeI (6250) RsrII (5943) BsrDI (5660) PflFI - Tth111I (5545) PmeI (5202) SanDI (5136) AscI - BssHII (5045) FseI (4899) SwaI (4793) AlwNI (4320) ApaLI (4218) SwaI (3900) PacI (3774) 3' β-globin insulator PstI - SbfI (3642) MfeI (3305) AanI - PsiI (3276) BtsI (3222) AsiSI - PvuI (5) BglII (232) CMV enhancer SnaBI (566) BglII (816) NotI (858) Eco53kI (879) SacI (881) EcoRI (885) NcoI (905) EcoRV (921) AbsI - PaeR7I - PspXI - XhoI (928) BsgI (940) BspDI - ClaI (977) EcoNI (1011) PspOMI (1226) ApaI (1230) PflMI (1308) AhdI (1438) BbsI (1531) PvuII (1547) ScaI (1638) BsrGI (1963) FspI (2295) KasI (2420) NarI (2421) SfoI (2422) PluTI (2424) BlpI (2427) AgeI (2604) DraIII (2619) SexAI * (2812) BpmI (2853) NheI (3126) BmtI (3130) stop codons pSF-CMV-FMDV-FLuc 6369 bp
PmeI  (6250)
2 sites
G T T T A A A C C A A A T T T G
RsrII  (5943)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (5660)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (5545)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5545)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PmeI  (5202)
2 sites
G T T T A A A C C A A A T T T G
SanDI  (5136)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
AscI  (5045)
1 site
G G C G C G C C C C G C G C G G
BssHII  (5045)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
FseI  (4899)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
SwaI  (4793)
2 sites
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
AlwNI  (4320)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
ApaLI  (4218)
1 site
G T G C A C C A C G T G
SwaI  (3900)
2 sites
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
PacI  (3774)
1 site
T T A A T T A A A A T T A A T T
PstI  (3642)
1 site
C T G C A G G A C G T C
SbfI  (3642)
1 site
C C T G C A G G G G A C G T C C
MfeI  (3305)
1 site
C A A T