pGL4.20[luc2 Puro]

Promoterless vector for measuring the activity of promoter and enhancer sequences with a luciferase assay.

Sequence Author: Promega

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Luciferase Vectors | More Plasmid Sets
No matches
AarI - BfuAI - BspMI (5374) RVprimer3 (5353 .. 5372) poly(A) signal BsmBI (5201) SpeI (5188) BstZ17I (4869) SacII (4753) PvuI (4729) Bsu36I (4715) AhdI (4359) BstEII (4284) BstXI - PstI (4281) NotI (4257) ApaLI (3751) BciVI (3640) AflIII - PciI (3437) RVprimer4 (3238 .. 3257) SalI (3187) BstBI (3173) BglI - SfiI (8) Acc65I (14) KpnI (18) Eco53kI (23) SacI (25) NheI (27) BmtI (31) AbsI - PaeR7I - PspXI - XhoI (33) MCS EcoRV (41) BglII (46) BglI - SfiI (59) HindIII (65) PspOMI (126) ApaI (130) MreI (163) BsrGI (590) BbvCI (811) BpmI (1480) FseI (1776) ApoI (1862) PsiI (1897) MfeI (1926) BamHI (2019) StuI (2452) NruI (2536) pGL4.20 [luc2/Puro] 5404 bp
AarI  (5374)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Cleavage may be enhanced when more than one copy of the AarI recognition sequence is present.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BfuAI  (5374)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50Ā°C, but is 50% active at 37Ā°C.
BspMI  (5374)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BsmBI  (5201)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
SpeI  (5188)
1 site
A C T A G T T G A T C A
BstZ17I  (4869)
1 site
G T A T A C C A T A T G
SacII  (4753)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PvuI  (4729)
1 site
C G A T C G G C T A G C
Bsu36I  (4715)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
AhdI  (4359)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BstEII  (4284)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60Ā°C, but is 50% active at 37Ā°C.
BstXI  (4281)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
PstI  (4281)
1 site
C T G C A G G A C G T C
NotI  (4257)
1 site
G C G G C C G C C G C C G G C G
ApaLI  (3751)
1 site
G T G C A C C A C G T G
BciVI  (3640)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
AflIII  (3437)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (3437)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
SalI  (3187)
1 site
G T C G A C C A G C T G
BstBI  (3173)
1 site
T T C G A A A A G C T T
BglI  (8)
2 sites
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfiI  (8)
2 sites
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
Acc65I  (14)
1 site
G G T A C C C C A T G G
KpnI  (18)
1 site
G G T A C C C C A T G G
Eco53kI  (23)
1 site
G A G C T C C T C G A G
SacI  (25)
1 site
G A G C T C C T C G A G
NheI  (27)
1 site
G C T A G C C G A T C G
BmtI  (31)
1 site
G C T A G C C G A T C G
AbsI  (33)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (33)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (33)
1 site
V C T C G A G B B G A G C T C V
XhoI  (33)
1 site
C T C G A G G A G C T C
EcoRV  (41)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BglII  (46)
1 site
A G A T C T T C T A G A
BglI  (59)
2 sites
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfiI  (59)
2 sites
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
HindIII  (65)
1 site
A A G C T T T T C G A A
PspOMI  (126)
1 site
G G G C C C C C C G G G
ApaI  (130)
1 site
G G G C C C C C C G G G

ApaI can be used between 25Ā°C and 37Ā°C.
MreI  (163)
1 site
C G C C G G C G G C G G C C G C
BsrGI  (590)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37Ā°C, but is even more active at 60Ā°C.
BbvCI  (811)
1 site
C C T C A G C G G A G T C G
BpmI  (1480)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37Ā°C.
FseI  (1776)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20Ā°C.
ApoI  (1862)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50Ā°C, but is 50% active at 37Ā°C.
PsiI  (1897)
1 site
T T A T A A A A T A T T
MfeI  (1926)
1 site
C A A T T G G T T A A C
BamHI  (2019)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HFĀ® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
StuI  (2452)
1 site
A G G C C T T C C G G A
NruI  (2536)
1 site
T C G C G A A G C G C T
RVprimer3
20-mer  /  50% GC
1 binding site
5353 .. 5372  =  20 annealed bases
Tm  =  54Ā°C
RVprimer4
20-mer  /  65% GC
1 binding site
3238 .. 3257  =  20 annealed bases
Tm  =  62Ā°C
luciferase
100 .. 1752  =  1653 bp
550 amino acids  =  60.6 kDa
Product: firefly luciferase
synthetic luc2 version of the luciferase gene
luciferase
100 .. 1752  =  1653 bp
550 amino acids  =  60.6 kDa
Product: firefly luciferase
synthetic luc2 version of the luciferase gene
AmpR
4286 .. 5146  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4286 .. 5077  =  792 bp
   263 amino acids  =  28.9 kDa
Product: Ī²-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4286 .. 5146  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5078 .. 5146  =  69 bp
   23 amino acids  =  2.6 kDa
Product: Ī²-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4286 .. 5146  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: Ī²-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
PuroR
2499 .. 3098  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
2499 .. 3098  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
ori
3498 .. 4086  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3498 .. 4086  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
SV40 promoter
2111 .. 2468  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
2111 .. 2468  =  358 bp
SV40 enhancer and early promoter
SV40 poly(A) signal
1796 .. 1917  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1796 .. 1917  =  122 bp
SV40 polyadenylation signal
pause site
5313 .. 5404  =  92 bp
RNA polymerase II transcriptional pause signal from the human Ī±2 globin gene
pause site
5313 .. 5404  =  92 bp
RNA polymerase II transcriptional pause signal from the human Ī±2 globin gene
MCS
1 .. 70  =  70 bp
multiple cloning site
MCS
1 .. 70  =  70 bp
multiple cloning site
poly(A) signal
3123 .. 3171  =  49 bp
synthetic polyadenylation signal
poly(A) signal
3123 .. 3171  =  49 bp
synthetic polyadenylation signal
poly(A) signal
5251 .. 5299  =  49 bp
synthetic polyadenylation signal
poly(A) signal
5251 .. 5299  =  49 bp
synthetic polyadenylation signal
ORF:  100 .. 1752  =  1653 bp
ORF:  550 amino acids  =  60.6 kDa
ORF:  2499 .. 3098  =  600 bp
ORF:  199 amino acids  =  21.5 kDa
ORF:  4416 .. 4682  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  4286 .. 5146  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  43 .. 327  =  285 bp
ORF:  94 amino acids  =  10.0 kDa
ORF:  2728 .. 3006  =  279 bp
ORF:  92 amino acids  =  10.6 kDa
ORF:  2454 .. 3116  =  663 bp
ORF:  220 amino acids  =  21.9 kDa
Click here to try SnapGene

Download pGL4.20[luc2 Puro].dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ā€™ā€™www.snapgene.com/resourcesā€™ā€™. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.