pGL4.49[luc2P TCF-LEF RE Hygro]
Vector with a minimal promoter and a TCF/LEF response element for studying cell signaling using destabilized luciferase.
Sequence Author: Promega
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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Sticky ends from different Bsu36I sites may not be compatible. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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Sticky ends from different BstXI sites may not be compatible. |
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Sticky ends from different AlwNI sites may not be compatible. |
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The 1-base overhangs produced by BciVI may be hard to ligate.Sticky ends from different BciVI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
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Cleavage may be enhanced when more than one copy of the AarI recognition sequence is present. Sticky ends from different AarI sites may not be compatible.After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility. |
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Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
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Sticky ends from different BglI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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Sticky ends from different BglI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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Sticky ends from different BlpI sites may not be compatible. |
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Sticky ends from different DraIII sites may not be compatible. |
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Efficient cleavage requires at least two copies of the BpmI recognition sequence. Sticky ends from different BpmI sites may not be compatible.After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.BpmI quickly loses activity at 37°C. |
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FseI gradually loses activity when stored at -20°C. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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| RVprimer4 20-mer / 65% GC 1 binding site 4216 .. 4235 = 20 annealed bases Tm = 62°C |
| RVprimer3 20-mer / 50% GC 1 binding site 207 .. 226 = 20 annealed bases Tm = 54°C |
| luciferase 513 .. 2162 = 1650 bp 550 amino acids = 60.6 kDa Product: firefly luciferase synthetic luc2 version of the luciferase gene |
| luciferase 513 .. 2162 = 1650 bp 550 amino acids = 60.6 kDa Product: firefly luciferase synthetic luc2 version of the luciferase gene |
| hPEST 2166 .. 2285 = 120 bp 40 amino acids = 4.2 kDa Product: PEST degradation sequence from mouse ornithine decarboxylase human codon-optimized |
| hPEST 2166 .. 2285 = 120 bp 40 amino acids = 4.2 kDa Product: PEST degradation sequence from mouse ornithine decarboxylase human codon-optimized |
| HygR 3040 .. 4077 = 1038 bp 345 amino acids = 38.4 kDa Product: hygromycin B phosphotransferase confers resistance to hygromycin |
| HygR 3040 .. 4077 = 1038 bp 345 amino acids = 38.4 kDa Product: hygromycin B phosphotransferase confers resistance to hygromycin |
| AmpR 5264 .. 6124 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 5264 .. 6055 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 5264 .. 6124 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 6056 .. 6124 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 5264 .. 6124 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| ori 4476 .. 5064 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 4476 .. 5064 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| SV40 promoter 2652 .. 3009 = 358 bp SV40 enhancer and early promoter |
| SV40 promoter 2652 .. 3009 = 358 bp SV40 enhancer and early promoter |
| SV40 poly(A) signal 2337 .. 2458 = 122 bp SV40 polyadenylation signal |
| SV40 poly(A) signal 2337 .. 2458 = 122 bp SV40 polyadenylation signal |
| TCF/LEF RE 285 .. 404 = 120 bp TCF/LEF response element (8 copies) |
| TCF/LEF RE 285 .. 404 = 120 bp TCF/LEF response element (8 copies) |
| pause site 167 .. 258 = 92 bp RNA polymerase II transcriptional pause signal from the human α2 globin gene |
| pause site 167 .. 258 = 92 bp RNA polymerase II transcriptional pause signal from the human α2 globin gene |