pBIND-GR

Renilla luciferase vector encoding the Gal4 DNA-binding domain fused to the glucocorticoid receptor ligand-binding domain.

Sequence Author: Promega

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HomePlasmidsLuciferase VectorspBIND-GR
BglII (7543) MluI (7424) AhdI (6277) BspDI - ClaI (5232) poly(A) signal BstXI (5172) RsrII (4967) NaeI (4953) NgoMIV (4951) BssHII (4848) PluTI (4454) SfoI (4452) NarI (4451) KasI (4450) EcoO109I (4187) PasI (4116) NruI (4003) EcoRV (3827) SpeI (152) CMV enhancer NdeI (387) T7 promoter NheI (1052) BmtI (1056) PaeR7I - XhoI (1300) GAL4 DNA binding domain AsiSI - PvuI (1558) XcmI (1729) DraI - PmeI (2403) TspMI - XmaI (2424) SmaI (2426) XbaI (2435) SbfI (2451) BlpI (2516) NotI (2546) PsiI (2669) MfeI (2698) SfiI (3229) StuI (3275) AvrII (3276) PflMI (3330) DraIII (3530) pBIND-GR 7548 bp
BglII  (7543)
1 site		 A G A T C T T C T A G A
MluI  (7424)
1 site		 A C G C G T T G C G C A
AhdI  (6277)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BspDI  (5232)
1 site		 A T C G A T T A G C T A
ClaI  (5232)
1 site		 A T C G A T T A G C T A
BstXI  (5172)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
RsrII  (4967)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
NaeI  (4953)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (4951)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BssHII  (4848)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
PluTI  (4454)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (4452)
1 site		 G G C G C C C C G C G G
NarI  (4451)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (4450)
1 site		 G G C G C C C C G C G G
EcoO109I  (4187)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
PasI  (4116)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
NruI  (4003)
1 site		 T C G C G A A G C G C T
EcoRV  (3827)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
SpeI  (152)
1 site		 A C T A G T T G A T C A
NdeI  (387)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
NheI  (1052)
1 site		 G C T A G C C G A T C G
BmtI  (1056)
1 site		 G C T A G C C G A T C G
PaeR7I  (1300)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1300)
1 site		 C T C G A G G A G C T C
AsiSI  (1558)
1 site		 G C G A T C G C C G C T A G C G
PvuI  (1558)
1 site		 C G A T C G G C T A G C
XcmI  (1729)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
DraI  (2403)
1 site		 T T T A A A A A A T T T
PmeI  (2403)
1 site		 G T T T A A A C C A A A T T T G
TspMI  (2424)
1 site		 C C C G G G G G G C C C
XmaI  (2424)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (2426)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
XbaI  (2435)
1 site		 T C T A G A A G A T C T
SbfI  (2451)
1 site		 C C T G C A G G G G A C G T C C
BlpI  (2516)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
NotI  (2546)
1 site		 G C G G C C G C C G C C G G C G
PsiI  (2669)
1 site		 T T A T A A A A T A T T
MfeI  (2698)
1 site		 C A A T T G G T T A A C
SfiI  (3229)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (3275)
1 site		 A G G C C T T C C G G A
AvrII  (3276)
1 site		 C C T A G G G G A T C C
PflMI  (3330)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
DraIII  (3530)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
hRluc
3342 .. 4274  =  933 bp
311 amino acids  =  36.0 kDa
Product: Renilla luciferase
human codon-optimized
hRluc
3342 .. 4274  =  933 bp
311 amino acids  =  36.0 kDa
Product: Renilla luciferase
human codon-optimized
NeoR/KanR
4326 .. 5117  =  792 bp
263 amino acids  =  28.9 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
4326 .. 5117  =  792 bp
263 amino acids  =  28.9 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
GAL4 DNA binding domain
1083 .. 1520  =  438 bp
146 amino acids  =  16.8 kDa
Product: DNA binding domain of the GAL4 transcriptional activator
GAL4 DNA binding domain
1083 .. 1520  =  438 bp
146 amino acids  =  16.8 kDa
Product: DNA binding domain of the GAL4 transcriptional activator
IgA linker
1527 .. 1553  =  27 bp
9 amino acids  =  880.0 Da
Product: peptide derived from a human Ig alpha chain
IgA linker
1527 .. 1553  =  27 bp
9 amino acids  =  880.0 Da
Product: peptide derived from a human Ig alpha chain
GR-LBD
1563 .. 2399  =  837 bp
278 amino acids  =  32.0 kDa
Product: glucocorticoid nuclear receptor ligand binding domain
GR-LBD
1563 .. 2399  =  837 bp
278 amino acids  =  32.0 kDa
Product: glucocorticoid nuclear receptor ligand binding domain
AmpR
5490 .. 6350  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5490 .. 5558  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5490 .. 6350  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   5559 .. 6350  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
5490 .. 6350  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
6508 .. 7096  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6508 .. 7096  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
SV40 promoter
2934 .. 3291  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
2934 .. 3291  =  358 bp
SV40 enhancer and early promoter
CMV enhancer
214 .. 517  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
214 .. 517  =  304 bp
human cytomegalovirus immediate early enhancer
cer region
7212 .. 7495  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
cer region
7212 .. 7495  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
CMV promoter
518 .. 721  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
518 .. 721  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
chimeric intron
857 .. 989  =  133 bp
chimera between introns from human β-globin and immunoglobulin heavy chain genes
chimeric intron
857 .. 989  =  133 bp
chimera between introns from human β-globin and immunoglobulin heavy chain genes
SV40 poly(A) signal
2568 .. 2689  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2568 .. 2689  =  122 bp
SV40 polyadenylation signal
AmpR promoter
5385 .. 5489  =  105 bp
AmpR promoter
5385 .. 5489  =  105 bp
poly(A) signal
5181 .. 5229  =  49 bp
synthetic polyadenylation signal
poly(A) signal
5181 .. 5229  =  49 bp
synthetic polyadenylation signal
T7 promoter
1033 .. 1051  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1033 .. 1051  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
3142 .. 3277  =  136 bp
SV40 origin of replication
SV40 ori
3142 .. 3277  =  136 bp
SV40 origin of replication
ORF:  4495 .. 4881  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  1083 .. 2399  =  1317 bp
ORF:  438 amino acids  =  50.1 kDa
ORF:  3342 .. 5117  =  1776 bp
ORF:  591 amino acids  =  66.0 kDa
ORF:  5490 .. 6350  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  4993 .. 5298  =  306 bp
ORF:  101 amino acids  =  11.5 kDa
ORF:  3207 .. 3494  =  288 bp
ORF:  95 amino acids  =  10.2 kDa
ORF:  4632 .. 5168  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  1565 .. 2002  =  438 bp
ORF:  145 amino acids  =  15.9 kDa
ORF:  5954 .. 6220  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Individual Sequences & Maps

pBI-CMV5
pGL4.22[luc2CP Puro]
pGL4.81[hRlucCP Neo]
pRL-null
pBIND-ER(alpha)
pGL4.23[luc2 minP]
pGL4.82[hRluc Puro]
pRL-SV40
pBIND-GR
pGL4.24[luc2P minP]
pGL4.83[hRlucP Puro]
pRL-TK
pCBG68-Basic
pGL4.25[luc2CP minP]
pGL4.84[hRlucCP Puro]
pSELECT-zeo-Lucia
pCBG68-Control
pGL4.26[luc2 minP Hygro]
pGLuc Mini-TK 2
pSF-CMV-EMCV-FLuc
pCBG99-Basic
pGL4.27[luc2P minP Hygro]
pGLuc-Basic 2
pSF-CMV-EMCV-RLuc
pCBG99-Control
pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc