pGL4.83[hRlucP Puro]
Promoterless vector encoding destabilized Renilla luciferase for measuring the activity of promoter and enhancer sequences.
Sequence Author: Promega
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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Cleavage may be enhanced when more than one copy of the AarI recognition sequence is present. Sticky ends from different AarI sites may not be compatible.After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility. |
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Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
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Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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Sticky ends from different BstXI sites may not be compatible. |
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The 1-base overhangs produced by BciVI may be hard to ligate.Sticky ends from different BciVI sites may not be compatible. |
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Sticky ends from different AflIII sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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Sticky ends from different BglI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present. This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.Sticky ends from different Bpu10I sites may not be compatible. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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Sticky ends from different BglI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible. |
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Sticky ends from different BglI sites may not be compatible. |
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* Blocked by Dcm methylation. Sticky ends from different PfoI sites may not be compatible. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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Sticky ends from different PasI sites may not be compatible. |
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FseI gradually loses activity when stored at -20°C. |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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Sticky ends from different BtgZI sites may not be compatible.After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.BtgZI is typically used at 60°C, but is 75% active at 37°C. |
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Sticky ends from different BlpI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
RVprimer3 20-mer / 50% GC 1 binding site 4764 .. 4783 = 20 annealed bases Tm = 54°C |
RVprimer4 20-mer / 65% GC 1 binding site 2649 .. 2668 = 20 annealed bases Tm = 62°C |
hRluc 100 .. 1032 = 933 bp 311 amino acids = 36.0 kDa Product: Renilla luciferase human codon-optimized |
hRluc 100 .. 1032 = 933 bp 311 amino acids = 36.0 kDa Product: Renilla luciferase human codon-optimized |
hPEST 1036 .. 1155 = 120 bp 40 amino acids = 4.2 kDa Product: PEST degradation sequence from mouse ornithine decarboxylase human codon-optimized |
hPEST 1036 .. 1155 = 120 bp 40 amino acids = 4.2 kDa Product: PEST degradation sequence from mouse ornithine decarboxylase human codon-optimized |
AmpR 3697 .. 4557 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 3697 .. 4488 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 3697 .. 4557 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 4489 .. 4557 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 3697 .. 4557 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
PuroR 1910 .. 2509 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
PuroR 1910 .. 2509 = 600 bp 199 amino acids = 21.5 kDa Product: puromycin N-acetyltransferase confers resistance to puromycin |
ori 2909 .. 3497 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 2909 .. 3497 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
SV40 promoter 1522 .. 1879 = 358 bp SV40 enhancer and early promoter |
SV40 promoter 1522 .. 1879 = 358 bp SV40 enhancer and early promoter |
SV40 poly(A) signal 1207 .. 1328 = 122 bp SV40 polyadenylation signal |
SV40 poly(A) signal 1207 .. 1328 = 122 bp SV40 polyadenylation signal |
pause site 4724 .. 4815 = 92 bp RNA polymerase II transcriptional pause signal from the human α2 globin gene |
pause site 4724 .. 4815 = 92 bp RNA polymerase II transcriptional pause signal from the human α2 globin gene |
MCS 1 .. 70 = 70 bp multiple cloning site |
MCS 1 .. 70 = 70 bp multiple cloning site |
poly(A) signal 2534 .. 2582 = 49 bp synthetic polyadenylation signal |
poly(A) signal 2534 .. 2582 = 49 bp synthetic polyadenylation signal |
poly(A) signal 4662 .. 4710 = 49 bp synthetic polyadenylation signal |
poly(A) signal 4662 .. 4710 = 49 bp synthetic polyadenylation signal |
SV40 ori 1730 .. 1865 = 136 bp SV40 origin of replication |
SV40 ori 1730 .. 1865 = 136 bp SV40 origin of replication |
ORF: 100 .. 1158 = 1059 bp ORF: 352 amino acids = 40.2 kDa |
ORF: 1910 .. 2509 = 600 bp ORF: 199 amino acids = 21.5 kDa |
ORF: 3827 .. 4093 = 267 bp ORF: 88 amino acids = 9.3 kDa |
ORF: 3697 .. 4557 = 861 bp ORF: 286 amino acids = 31.6 kDa |
ORF: 2139 .. 2417 = 279 bp ORF: 92 amino acids = 10.6 kDa |
ORF: 1865 .. 2527 = 663 bp ORF: 220 amino acids = 21.9 kDa |
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