pGL4.83[hRlucP Puro]

Promoterless vector encoding destabilized Renilla luciferase for measuring the activity of promoter and enhancer sequences.

Sequence Author: Promega

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Luciferase Vectors | More Plasmid Sets
No matches
BsgI (4813) AarI - BfuAI - BspMI (4785) RVprimer3 (4764 .. 4783) poly(A) signal BsmBI (4612) SpeI (4599) BstZ17I (4280) SacII (4164) PvuI (4140) AhdI (3770) BstEII (3695) BstXI - PstI (3692) AleI (3690) NotI (3668) BspHI (3568) ApaLI (3162) BciVI (3051) BssSI (3021) AflIII - PciI (2848) AfeI (2724) BbsI (2677) RVprimer4 (2649 .. 2668) SalI (2598) BstBI (2584) AgeI (2519) BglI - SfiI (8) Acc65I (14) KpnI (18) Bpu10I (19) Eco53kI (23) SacI (25) NheI (27) BmtI (31) AbsI - PaeR7I - PspXI - XhoI (33) BglII (46) BglI - SfiI (59) HindIII (65) BglI (100) PfoI * (380) XmnI (634) BsaI (640) ZraI (795) PflFI - Tth111I (796) AatII (797) PasI (874) EcoRI (1032) FseI (1187) AanI - PsiI (1308) HpaI (1328) MfeI (1337) BamHI (1430) FspI (1468) PvuII (1540) SV40 promoter StuI (1863) BtgZI (1962) BlpI (2201) SgrAI (2369) pGL4.83[hRlucP/Puro] 4815 bp
BsgI  (4813)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AarI  (4785)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Cleavage may be enhanced when more than one copy of the AarI recognition sequence is present.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
BfuAI  (4785)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (4785)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BsmBI  (4612)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
SpeI  (4599)
1 site
A C T A G T T G A T C A
BstZ17I  (4280)
1 site
G T A T A C C A T A T G
SacII  (4164)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PvuI  (4140)
1 site
C G A T C G G C T A G C
AhdI  (3770)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BstEII  (3695)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstXI  (3692)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
PstI  (3692)
1 site
C T G C A G G A C G T C
AleI  (3690)
1 site
C A C N N N N G T G G T G N N N N C A C
NotI  (3668)
1 site
G C G G C C G C C G C C G G C G
BspHI  (3568)
1 site
T C A T G A A G T A C T
ApaLI  (3162)
1 site
G T G C A C C A C G T G
BciVI  (3051)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BssSI  (3021)
1 site
C A C G A G G T G C T C
AflIII  (2848)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2848)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AfeI  (2724)
1 site
A G C G C T T C G C G A
BbsI  (2677)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
SalI  (2598)
1 site
G T C G A C C A G C T G
BstBI  (2584)
1 site
T T C G A A A A G C T T
AgeI  (2519)
1 site
A C C G G T T G G C C A
BglI  (8)
3 sites
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfiI  (8)
2 sites
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
Acc65I  (14)
1 site
G G T A C C C C A T G G
KpnI  (18)
1 site
G G T A C C C C A T G G
Bpu10I  (19)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
Eco53kI  (23)
1 site
G A G C T C C T C G A G
SacI  (25)
1 site
G A G C T C C T C G A G
NheI  (27)
1 site
G C T A G C C G A T C G
BmtI  (31)
1 site
G C T A G C C G A T C G
AbsI  (33)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (33)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (33)
1 site
V C T C G A G B B G A G C T C V
XhoI  (33)
1 site
C T C G A G G A G C T C
BglII  (46)
1 site
A G A T C T T C T A G A
BglI  (59)
3 sites
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfiI  (59)
2 sites
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
HindIII  (65)
1 site
A A G C T T T T C G A A
BglI  (100)
3 sites
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
PfoI  (380)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
XmnI  (634)
1 site
G A A N N N N T T C C T T N N N N A A G
BsaI  (640)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
ZraI  (795)
1 site
G A C G T C C T G C A G
PflFI  (796)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (796)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
AatII  (797)
1 site
G A C G T C C T G C A G
PasI  (874)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
EcoRI  (1032)
1 site
G A A T T C C T T A A G
FseI  (1187)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
AanI  (1308)
1 site
T T A T A A A A T A T T
PsiI  (1308)
1 site
T T A T A A A A T A T T
HpaI  (1328)
1 site
G T T A A C C A A T T G
MfeI  (1337)
1 site
C A A T T G G T T A A C
BamHI  (1430)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
FspI  (1468)
1 site
T G C G C A A C G C G T
PvuII  (1540)
1 site
C A G C T G G T C G A C
StuI  (1863)
1 site
A G G C C T T C C G G A
BtgZI  (1962)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BlpI  (2201)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
SgrAI  (2369)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
RVprimer3
20-mer  /  50% GC
1 binding site
4764 .. 4783  =  20 annealed bases
Tm  =  54°C
RVprimer4
20-mer  /  65% GC
1 binding site
2649 .. 2668  =  20 annealed bases
Tm  =  62°C
hRluc
100 .. 1032  =  933 bp
311 amino acids  =  36.0 kDa
Product: Renilla luciferase
human codon-optimized
hRluc
100 .. 1032  =  933 bp
311 amino acids  =  36.0 kDa
Product: Renilla luciferase
human codon-optimized
hPEST
1036 .. 1155  =  120 bp
40 amino acids  =  4.2 kDa
Product: PEST degradation sequence from mouse ornithine decarboxylase
human codon-optimized
hPEST
1036 .. 1155  =  120 bp
40 amino acids  =  4.2 kDa
Product: PEST degradation sequence from mouse ornithine decarboxylase
human codon-optimized
AmpR
3697 .. 4557  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3697 .. 4488  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3697 .. 4557  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4489 .. 4557  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3697 .. 4557  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
PuroR
1910 .. 2509  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
1910 .. 2509  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
ori
2909 .. 3497  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2909 .. 3497  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
SV40 promoter
1522 .. 1879  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
1522 .. 1879  =  358 bp
SV40 enhancer and early promoter
SV40 poly(A) signal
1207 .. 1328  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1207 .. 1328  =  122 bp
SV40 polyadenylation signal
pause site
4724 .. 4815  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
pause site
4724 .. 4815  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
MCS
1 .. 70  =  70 bp
multiple cloning site
MCS
1 .. 70  =  70 bp
multiple cloning site
poly(A) signal
2534 .. 2582  =  49 bp
synthetic polyadenylation signal
poly(A) signal
2534 .. 2582  =  49 bp
synthetic polyadenylation signal
poly(A) signal
4662 .. 4710  =  49 bp
synthetic polyadenylation signal
poly(A) signal
4662 .. 4710  =  49 bp
synthetic polyadenylation signal
SV40 ori
1730 .. 1865  =  136 bp
SV40 origin of replication
SV40 ori
1730 .. 1865  =  136 bp
SV40 origin of replication
ORF:  100 .. 1158  =  1059 bp
ORF:  352 amino acids  =  40.2 kDa
ORF:  1910 .. 2509  =  600 bp
ORF:  199 amino acids  =  21.5 kDa
ORF:  3827 .. 4093  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  3697 .. 4557  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  2139 .. 2417  =  279 bp
ORF:  92 amino acids  =  10.6 kDa
ORF:  1865 .. 2527  =  663 bp
ORF:  220 amino acids  =  21.9 kDa
Click here to try SnapGene

Download pGL4.83[hRlucP Puro].dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
<