pSF-CMV-PGK-FLuc

Dual-promoter mammalian vector for strong constitutive expression of a gene together with firefly luciferase.

Sequence Author: Oxford Genetics

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PmeI (6275) RsrII (5968) PflFI - Tth111I (5570) PmeI (5227) SanDI (5161) AscI - BssHII (5070) FseI (4924) SwaI (4818) ApaLI (4243) SwaI (3925) PacI (3799) BstEII (3773) 3' β-globin insulator PstI - SbfI (3667) MfeI (3330) AanI - PsiI (3301) BtsI (3247) stop codons AsiSI - PvuI (5) BglII (232) CMV enhancer SnaBI (566) BglII (816) EagI - NotI (858) HindIII (869) Eco53kI (879) SacI (881) EcoRI (885) KpnI (901) NcoI (905) KpnI (915) EcoRV (921) AbsI - PaeR7I - PspXI - XhoI (928) XbaI (937) BseRI - BsgI (940) stop codons BspDI - ClaI (977) SphI (1027) BsmBI - SpeI (1233) BspEI (1407) BamHI (1488) BbsI (1556) PvuII (1572) ScaI (1663) BsrGI (1988) AleI (1996) FspI (2320) DraIII (2644) SexAI * (2837) NheI (3151) BmtI (3155) pSF-CMV-PGK-FLuc 6394 bp
PmeI  (6275)
2 sites
G T T T A A A C C A A A T T T G
RsrII  (5968)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PflFI  (5570)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5570)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PmeI  (5227)
2 sites
G T T T A A A C C A A A T T T G
SanDI  (5161)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
AscI  (5070)
1 site
G G C G C G C C C C G C G C G G
BssHII  (5070)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
FseI  (4924)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
SwaI  (4818)
2 sites
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
ApaLI  (4243)
1 site
G T G C A C C A C G T G
SwaI  (3925)
2 sites
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
PacI  (3799)
1 site
T T A A T T A A A A T T A A T T
BstEII  (3773)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PstI  (3667)
1 site
C T G C A G G A C G T C
SbfI  (3667)
1 site
C C T G C A G G G G A C G T C C
MfeI  (3330)
1 site
C A A T T G G T T A A C
AanI  (3301)
1 site
T T A T A A A A T A T T
PsiI  (3301)
1 site
T T A T A A A A T A T T
BtsI  (3247)
1 site
G C A G T G N N C G T C A C
AsiSI  (5)
1 site
G C G A T C G C C G C T A G C G
PvuI  (5)
1 site
C G A T C G G C T A G C
BglII  (232)
2 sites
A G A T C T T C T A G A
SnaBI  (566)
1 site
T A C G T A A T G C A T
BglII  (816)
2 sites
A G A T C T T C T A G A
EagI  (858)
1 site
C G G C