pSF-CMV-PGK-FLuc
Dual-promoter mammalian vector for strong constitutive expression of a gene together with firefly luciferase.
Sequence Author: Oxford Genetics
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
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The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
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Sticky ends from different SanDI sites may not be compatible. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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FseI gradually loses activity when stored at -20°C. |
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SwaI is typically used at 25°C, but is 50% active at 37°C. |
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SwaI is typically used at 25°C, but is 50% active at 37°C. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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