pSF-CMV-PGK-RLuc
Dual-promoter mammalian vector for strong constitutive expression of a gene together with Renilla luciferase.
Sequence Author: Oxford Genetics
Explore Over 2.7k Plasmids: Luciferase Vectors | More Plasmid Sets
No matches
|
| ||
Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
| ||
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
| ||
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
|
| ||
Sticky ends from different SanDI sites may not be compatible. |
|
| ||
FseI gradually loses activity when stored at -20°C. |
| ||
Efficient cleavage requires at least two copies of the NaeI recognition sequence. |
| ||
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. |
| ||
SwaI is typically used at 25°C, but is 50% active at 37°C. |
|
| ||
SwaI is typically used at 25°C, but is 50% active at 37°C. |
|
| ||
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| ||
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
|
| ||
PaeR7I does not recognize the sequence CTCTCGAG. |
|
|
|
|