pSF-CMV-FLuc
Mammalian vector for strong constitutive expression of firefly luciferase.
Sequence Author: Oxford Genetics
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Sticky ends from different PpuMI sites may not be compatible. |
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Sticky ends from different SanDI sites may not be compatible. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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FseI gradually loses activity when stored at -20°C. |
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SwaI is typically used at 25°C, but is 50% active at 37°C. |
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Sticky ends from different AlwNI sites may not be compatible. |
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SwaI is typically used at 25°C, but is 50% active at 37°C. |
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Sticky ends from different BanII sites may not be compatible. |
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Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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Efficient cleavage requires at least two copies of the NarI recognition sequence. |
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Efficient cleavage requires at least two copies of the PluTI recognition sequence. |
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Sticky ends from different BlpI sites may not be compatible. |
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Sticky ends from different DraIII sites may not be compatible. |
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* Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
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Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA. |
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
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luciferase 907 .. 2559 = 1653 bp 550 amino acids = 60.6 kDa Product: firefly luciferase synthetic luc2 version of the luciferase gene |
luciferase 907 .. 2559 = 1653 bp 550 amino acids = 60.6 kDa Product: firefly luciferase synthetic luc2 version of the luciferase gene |
AmpR 4793 .. 5653 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 1: signal sequence 4793 .. 4861 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 4793 .. 5653 = 861 bp 286 amino acids = 31.6 kDa 2 segments Segment 2: 4862 .. 5653 = 792 bp 263 amino acids = 28.9 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
AmpR 4793 .. 5653 = 861 bp 286 amino acids = 31.6 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
ori 3459 .. 4047 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
ori 3459 .. 4047 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
CMV enhancer 287 .. 590 = 304 bp human cytomegalovirus immediate early enhancer |
CMV enhancer 287 .. 590 = 304 bp human cytomegalovirus immediate early enhancer |
CMV promoter 591 .. 794 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
CMV promoter 591 .. 794 = 204 bp human cytomegalovirus (CMV) immediate early promoter |
rrnG terminator 2981 .. 3117 = 137 bp transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991) |
rrnG terminator 2981 .. 3117 = 137 bp transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991) |
rrnG terminator 5666 .. 5802 = 137 bp transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991) |
rrnG terminator 5666 .. 5802 = 137 bp transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991) |
SV40 poly(A) signal 2669 .. 2790 = 122 bp SV40 polyadenylation signal |
SV40 poly(A) signal 2669 .. 2790 = 122 bp SV40 polyadenylation signal |
5' β-globin insulator 18 .. 89 = 72 bp insulator upstream of the human β-globin locus (Farrell et al., 2002) |
5' β-globin insulator 18 .. 89 = 72 bp insulator upstream of the human β-globin locus (Farrell et al., 2002) |
3' β-globin insulator 3162 .. 3233 = 72 bp insulator downstream of the human β-globin locus (Farrell et al., 2002) |
3' β-globin insulator 3162 .. 3233 = 72 bp insulator downstream of the human β-globin locus (Farrell et al., 2002) |
T7 terminator 2905 .. 2951 = 47 bp transcription terminator for bacteriophage T7 RNA polymerase |
T7 terminator 2905 .. 2951 = 47 bp transcription terminator for bacteriophage T7 RNA polymerase |
stop codons 2579 .. 2589 = 11 bp stop codons in all three reading frames |
stop codons 2579 .. 2589 = 11 bp stop codons in all three reading frames |
stop codons 2627 .. 2637 = 11 bp stop codons in all three reading frames |
stop codons 2627 .. 2637 = 11 bp stop codons in all three reading frames |
RBS 893 .. 898 = 6 bp Shine-Dalgarno ribosome binding site |
RBS 893 .. 898 = 6 bp Shine-Dalgarno ribosome binding site |
RBS 4780 .. 4785 = 6 bp Shine-Dalgarno ribosome binding site |
RBS 4780 .. 4785 = 6 bp Shine-Dalgarno ribosome binding site |
Kozak sequence 903 .. 909 = 7 bp |
Kozak sequence 903 .. 909 = 7 bp |
ORF: 907 .. 2559 = 1653 bp ORF: 550 amino acids = 60.6 kDa |
ORF: 4793 .. 5653 = 861 bp ORF: 286 amino acids = 31.6 kDa |
ORF: 5684 .. 59 = 312 bp ORF: 103 amino acids = 11.6 kDa |
ORF: 3006 .. 3437 = 432 bp ORF: 143 amino acids = 16.3 kDa |
ORF: 889 .. 1134 = 246 bp ORF: 81 amino acids = 8.8 kDa |
ORF: 1903 .. 2163 = 261 bp ORF: 86 amino acids = 8.9 kDa |
ORF: 5257 .. 5523 = 267 bp ORF: 88 amino acids = 9.3 kDa |
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