pSF-CMV-Ub-RLuc AscI

Mammalian vector for co-expressing a gene together with Renilla luciferase expressed from the ubiquitin C promoter.

Sequence Author: Oxford Genetics

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HomePlasmidsLuciferase VectorspSF-CMV-Ub-RLuc AscI
AsiSI - PvuI (5) PmeI (6367) RsrII (6060) BsrDI (5777) PflFI - Tth111I (5662) PmeI (5319) AscI (5162) AanI - AanI (4434) BstAPI (4330) EcoNI (3869) AvrII (3865) AarI (3819) AanI - AanI (3792) BpmI (3688) BbsI (3509) BsaI (3394) DraIII (3325) AfeI (2984) BglII (232) SnaBI (566) CMV promoter BglII (816) EagI - NotI (858) HindIII (869) Eco53kI (879) SacI (881) EcoRI (885) MCS KpnI (901) NcoI (905) KpnI (915) EcoRV (921) AbsI - PaeR7I - PspXI - XhoI (928) XbaI (937) BseRI - BsgI (940) stop codons BspDI - ClaI (977) BamHI (986) StuI (996) NheI (1002) BmtI (1006) stop codons AanI - AanI (1152) HpaI (1172) MfeI (1181) T7 terminator PstI - SbfI (1518) 3' β-globin insulator BstEII (1624) PacI (1650) SwaI (1776) AlwNI (2196) BspHI (2500) SwaI (2669) NgoMIV (2771) NaeI (2773) FseI (2775) AscI (2921) SacII (2959) pSF-CMV-Ub-RLuc AscI 6486 bp
AsiSI  (5)
1 site		 G C G A T C G C C G C T A G C G
PvuI  (5)
1 site		 C G A T C G G C T A G C
PmeI  (6367)
2 sites		 G T T T A A A C C A A A T T T G
RsrII  (6060)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (5777)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
PflFI  (5662)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5662)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PmeI  (5319)
2 sites		 G T T T A A A C C A A A T T T G
AscI  (5162)
2 sites		 G G C G C G C C C C G C G C G G
AanI  (4434)
3 sites		 T T A T A A A A T A T T
AanI  (4434)
3 sites		 T T A T A A A A T A T T
BstAPI  (4330)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
EcoNI  (3869)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
AvrII  (3865)
1 site		 C C T A G G G G A T C C
AarI  (3819)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Cleavage may be enhanced when more than one copy of the AarI recognition sequence is present.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
AanI  (3792)
3 sites