pSF-CMV-Ub-RLuc AscI

Mammalian vector for co-expressing a gene together with Renilla luciferase expressed from the ubiquitin C promoter.

Sequence Author: Oxford Genetics

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HomePlasmidsLuciferase VectorspSF-CMV-Ub-RLuc AscI
AsiSI - PvuI (5) PmeI (6367) RsrII (6060) BsrDI (5777) PflFI - Tth111I (5662) PmeI (5319) AscI (5162) AanI - AanI (4434) BstAPI (4330) EcoNI (3869) AvrII (3865) AarI (3819) AanI - AanI (3792) BpmI (3688) BbsI (3509) BsaI (3394) DraIII (3325) AfeI (2984) BglII (232) SnaBI (566) CMV promoter BglII (816) EagI - NotI (858) HindIII (869) Eco53kI (879) SacI (881) EcoRI (885) MCS KpnI (901) NcoI (905) KpnI (915) EcoRV (921) AbsI - PaeR7I - PspXI - XhoI (928) XbaI (937) BseRI - BsgI (940) stop codons BspDI - ClaI (977) BamHI (986) StuI (996) NheI (1002) BmtI (1006) stop codons AanI - AanI (1152) HpaI (1172) MfeI (1181) T7 terminator PstI - SbfI (1518) 3' β-globin insulator BstEII (1624) PacI (1650) SwaI (1776) AlwNI (2196) BspHI (2500) SwaI (2669) NgoMIV (2771) NaeI (2773) FseI (2775) AscI (2921) SacII (2959) pSF-CMV-Ub-RLuc AscI 6486 bp
AsiSI  (5)
1 site		 G C G A T C G C C G C T A G C G
PvuI  (5)
1 site		 C G A T C G G C T A G C
PmeI  (6367)
2 sites		 G T T T A A A C C A A A T T T G
RsrII  (6060)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (5777)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
PflFI  (5662)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5662)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PmeI  (5319)
2 sites		 G T T T A A A C C A A A T T T G
AscI  (5162)
2 sites		 G G C G C G C C C C G C G C G G
AanI  (4434)
3 sites		 T T A T A A A A T A T T
AanI  (4434)
3 sites		 T T A T A A A A T A T T
BstAPI  (4330)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
EcoNI  (3869)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
AvrII  (3865)
1 site		 C C T A G G G G A T C C
AarI  (3819)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N )