pSF-CMV-RSV-RLuc AscI

Mammalian vector for co-expressing a gene together with Renilla luciferase expressed from the Rous sarcoma virus promoter.

Sequence Author: Oxford Genetics

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PmeI (5610) RsrII (5303) PflFI - Tth111I (4905) PmeI (4562) PpuMI - SanDI (4496) AscI (4405) AanI (3677) BstAPI (3573) AleI (3361) SphI (3202) Bsu36I (3070) NruI (2978) AscI (2921) AsiSI (5) BglII (232) CMV enhancer SnaBI (566) BglII (816) EagI - NotI (858) HindIII (869) Eco53kI (879) SacI (881) EcoRI (885) KpnI (901) NcoI (905) KpnI (915) EcoRV (921) AbsI - PaeR7I - PspXI - XhoI (928) XbaI (937) BseRI - BsgI (940) stop codons BspDI - ClaI (977) BamHI (986) StuI (996) NheI (1002) BmtI (1006) stop codons AanI (1152) HpaI (1172) T7 terminator PstI - SbfI (1518) 3' β-globin insulator BstEII (1624) PacI (1650) SwaI (1776) BssSI (1953) AlwNI (2196) BspHI (2500) SwaI (2669) NgoMIV (2771) NaeI (2773) FseI (2775) pSF-CMV-RSV-RLuc AscI 5729 bp
PmeI  (5610)
2 sites
G T T T A A A C C A A A T T T G
RsrII  (5303)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PflFI  (4905)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4905)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PmeI  (4562)
2 sites
G T T T A A A C C A A A T T T G
PpuMI  (4496)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SanDI  (4496)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
AscI  (4405)
2 sites
G G C G C G C C C C G C G C G G
AanI  (3677)
2 sites
T T A T A A A A T A T T
BstAPI  (3573)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
AleI  (3361)
1 site
C A C N N N N G T G G T G N N N N C A C
SphI  (3202)
1 site
G C A T G C C G T A C G
Bsu36I  (3070)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
NruI  (2978)
1 site
T C G C G A A G C G C T
AscI  (2921)
2 sites
G G C G C G C C C C G C G C G G
AsiSI  (5)
1 site
G C G A T C G C C G C T A G C G
BglII  (232)
2 sites
A G A T C T T C T A G A
SnaBI  (566)
1 site
T A C G T A A T G C A T
BglII  (816)
2 sites
A G A T C T T C T A G A
EagI  (858)
1 site
C G G C C G G C C G G C
NotI  (858)
1 site
G C G G C C G C C G C C G G C G
HindIII  (869)
1 site
A A G C T T T T C G A A
Eco53kI  (879)
1 site
G A G C T C C T C G A G
SacI  (881)
1 site
G A G C T C C T C G A G
EcoRI  (885)
1 site
G A A T T C C T T A A G
KpnI  (901)
2 sites
G G T A C C C C A T G G
NcoI  (905)
1 site
C C A T G G G G T A C C
KpnI  (915)
2 sites
G G T A C C C C A T G G
EcoRV  (921)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
AbsI  (928)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (928)
1 site
C T C