pSF-CMV-EMCV-RLuc

Mammalian vector for co-expressing a gene together with Renilla luciferase expressed from the encephalomyocarditis virus IRES.

Sequence Author: Oxford Genetics

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PmeI (5623) RsrII (5316) PflFI - Tth111I (4918) PmeI (4575) PpuMI - SanDI (4509) AscI (4418) FseI (4272) NaeI (4270) NgoMIV (4268) SwaI (4166) BspHI (3997) AlwNI (3693) BssSI (3450) SwaI (3273) PacI (3147) BstEII (3121) 3' β-globin insulator PstI - SbfI (3015) AsiSI - PvuI (5) BglII (232) CMV enhancer SnaBI (566) BglII (816) EagI - NotI (858) Eco53kI (879) SacI (881) EcoRI (885) NcoI (905) EcoRV (921) AbsI - PaeR7I - PspXI - XhoI (928) XbaI (937) BseRI - BsgI (940) stop codons BspDI - ClaI (977) AfeI (997) PspOMI (1101) ApaI (1105) AvrII (1139) PmlI (1304) AarI (1327) DraIII (1351) PflMI (1441) BmgBI (1530) BstAPI (1753) AanI (1857) Bpu10I (2213) NheI (2499) BmtI (2503) stop codons AanI (2649) HpaI (2669) MfeI (2678) pSF-CMV-EMCV-RLuc 5742 bp
PmeI  (5623)
2 sites
G T T T A A A C C A A A T T T G
RsrII  (5316)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PflFI  (4918)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4918)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PmeI  (4575)
2 sites
G T T T A A A C C A A A T T T G
PpuMI  (4509)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SanDI  (4509)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
AscI  (4418)
1 site
G G C G C G C C C C G C G C G G
FseI  (4272)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
NaeI  (4270)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (4268)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SwaI  (4166)
2 sites
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
BspHI  (3997)
1 site
T C A T G A A G T A C T
AlwNI  (3693)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BssSI  (3450)
1 site
C A C G A G G T G C T C
SwaI  (3273)
2 sites
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
PacI  (3147)
1 site
T T A A T T A A A A T T A A T T
BstEII  (3121)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PstI  (3015)
1 site
C T G C A G G A C G T C
SbfI  (3015)
1 site
C C T G C A G G G G A C G T C C
AsiSI  (5)
1 site
G C G A T C G C C G C T A G C G
PvuI  (5)
1 site
C G A T C G G C T A G C
BglII  (232)
2 sites
A G A T C T T C T A G A
SnaBI  (566)
1 site
T A C G T A A T G C A T
BglII  (816)
2 sites
A G A T C T T C T A G A
EagI  (858)
1 site
C G G C C G G C C G G C
NotI  (858)
1 site
G C G G C C G C C G C C G G C G
Eco53kI  (879)
1 site
G A G C T C C T C G A G
SacI  (881)
1 site
G A G C T C C T C G A G
EcoRI  (885)
1 site
G A A T T C C T T A A G
NcoI  (905)
1 site
C C A T G G G G T A C C
EcoRV  (921)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
AbsI  (928)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (928)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (928)
1 site
V C T C G A G B B G A G C T C V
XhoI  (928)
1 site
C T C G A G G A G C T C
XbaI  (937)
1 site
T C T A G A A G A T C T
BseRI  (940)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BsgI  (940)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BspDI  (977)
1 site
A T C G A T T A G C T A
ClaI  (977)
1 site
A T C G A T T A G C T A
AfeI  (997)
1 site
A G C G C T T C G C G A
PspOMI  (1101)
1 site
G G G C C C C C C G G G
ApaI  (1105)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
AvrII  (1139)
1 site
C C T A G G G G A