pSF-CMV-EMCV-FLuc

Mammalian vector for co-expressing a gene together with firefly luciferase expressed from the encephalomyocarditis virus IRES.

Sequence Author: Oxford Genetics

No matches

PmeI (6342)
2 sites

RsrII (6035)
1 site

Efficient cleavage requires at least two copies of the RsrII recognition sequence.

Sticky ends from different RsrII sites may not be compatible.

For full activity, add fresh DTT.

PflFI (5637)
1 site

The 1-base overhangs produced by PflFI may be hard to ligate.

Sticky ends from different PflFI sites may not be compatible.

Tth111I (5637)
1 site

The 1-base overhangs produced by Tth111I may be hard to ligate.

Sticky ends from different Tth111I sites may not be compatible.

PmeI (5294)
2 sites

PpuMI (5228)
1 site

Sticky ends from different PpuMI sites may not be compatible.

SanDI (5228)
1 site

Sticky ends from different SanDI sites may not be compatible.

AscI (5137)
1 site

BssHII (5137)
1 site

BssHII is typically used at 50°C, but is 75% active at 37°C.

FseI (4991)
1 site

FseI gradually loses activity when stored at -20°C.

SwaI (4885)
2 sites

SwaI is typically used at 25°C, but is 50% active at 37°C.

AlwNI (4412)
1 site

Sticky ends from different AlwNI sites may not be compatible.

SwaI (3992)
2 sites

SwaI is typically used at 25°C, but is 50% active at 37°C.

PacI (3866)
1 site

BstEII (3840)
1 site

Sticky ends from different BstEII sites may not be compatible.

BstEII is typically used at 60°C, but is 50% active at 37°C.

PstI (3734)
1 site

SbfI (3734)
1 site

MfeI (3397)
1 site

AanI (3368)
1 site

PsiI (3368)
1 site

BtsI (3314)
1 site

AsiSI (5)
1 site