Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT.
PflFI (5637) 1 site
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible.
Tth111I (5637) 1 site
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible.
PmeI (5294) 2 sites
PpuMI (5228) 1 site
Sticky ends from different PpuMI sites may not be compatible.
SanDI (5228) 1 site
Sticky ends from different SanDI sites may not be compatible.
AscI (5137) 1 site
BssHII (5137) 1 site
BssHII is typically used at 50°C, but is 75% active at 37°C.
FseI (4991) 1 site
FseI gradually loses activity when stored at -20°C.
