pSELECT-zeo-Lucia

Mammalian vector for the expression of secreted Lucia® luciferase.

Sequence Author: InvivoGen

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NotI (2) BciVI (3224) BssSI - BssSαI (3194) DrdI (3129) NspI (3025) AflIII - PciI (3021) PstI - SbfI (3008) NdeI (2826) BsaAI - SnaBI (2722) SpeI (2592) SacI (2498) Eco53kI (2496) BsmBI - Esp3I (2469) AseI (2438) SfiI (2384) BssHII - MauBI (2341) BsrBI (2327) SmaI (2291) TspMI - XmaI (2289) SgrAI (2263) BmgBI (2247) CsiI - SexAI * (2213) NmeAIII (2133) FseI (2109) DraIII (2031) BseRI (2008) AsiSI - PvuI (176) AclI (370) PvuII (407) HindIII (411) KasI (701) NarI (702) SfoI (703) PluTI (705) AgeI (718) BstEII (721) NcoI (726) BbvCI (771) BbsI (804) BglII (841) EcoRV (1138) NheI (1360) BmtI (1364) BtsI - BtsαI (1426) PsiI (1480) HpaI (1500) EcoRI (1594) BspQI - EarI - SapI (1783) SspI (1835) pSELECT-zeo-Lucia 3769 bp
NotI  (2)
1 site
G C G G C C G C C G C C G G C G
BciVI  (3224)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BssSI  (3194)
1 site
C A C G A G G T G C T C
BssSαI  (3194)
1 site
C A C G A G G T G C T C
DrdI  (3129)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
NspI  (3025)
1 site
R C A T G Y Y G T A C R
AflIII  (3021)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (3021)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PstI  (3008)
1 site
C T G C A G G A C G T C
SbfI  (3008)
1 site
C C T G C A G G G G A C G T C C
NdeI  (2826)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BsaAI  (2722)
1 site
Y A C G T R R T G C A Y
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SnaBI  (2722)
1 site