pSELECT-zeo-Lucia

Mammalian vector for the expression of secreted Lucia® luciferase.

Sequence Author: InvivoGen

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HomePlasmidsLuciferase VectorspSELECT-zeo-Lucia
NotI (2) BciVI (3224) BssSI - BssSαI (3194) DrdI (3129) NspI (3025) AflIII - PciI (3021) PstI - SbfI (3008) NdeI (2826) BsaAI - SnaBI (2722) SpeI (2592) SacI (2498) Eco53kI (2496) BsmBI - Esp3I (2469) AseI (2438) SfiI (2384) BssHII - MauBI (2341) BsrBI (2327) SmaI (2291) TspMI - XmaI (2289) SgrAI (2263) BmgBI (2247) CsiI - SexAI * (2213) NmeAIII (2133) FseI (2109) DraIII (2031) BseRI (2008) AsiSI - PvuI (176) AclI (370) PvuII (407) HindIII (411) KasI (701) NarI (702) SfoI (703) PluTI (705) AgeI (718) BstEII (721) NcoI (726) BbvCI (771) BbsI (804) BglII (841) EcoRV (1138) NheI (1360) BmtI (1364) BtsI - BtsαI (1426) PsiI (1480) HpaI (1500) EcoRI (1594) BspQI - EarI - SapI (1783) SspI (1835) pSELECT-zeo-Lucia 3769 bp
NotI  (2)
1 site		 G C G G C C G C C G C C G G C G
BciVI  (3224)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BssSI  (3194)
1 site		 C A C G A G G T G C T C
BssSαI  (3194)
1 site		 C A C G A G G T G C T C
DrdI  (3129)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
NspI  (3025)
1 site		 R C A T G Y Y G T A C R
AflIII  (3021)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (3021)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
PstI  (3008)
1 site		 C T G C A G G A C G T C
SbfI  (3008)
1 site		 C C T G C A G G G G A C G T C C
NdeI  (2826)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BsaAI  (2722)
1 site		 Y A C G T R R T G C A Y
SnaBI  (2722)
1 site		 T A C G T A A T G C A T
SpeI  (2592)
1 site		 A C T A G T T G A T C A
SacI  (2498)
1 site		 G A G C T C C T C G A G
Eco53kI  (2496)
1 site		 G A G C T C C T C G A G
BsmBI  (2469)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (2469)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
AseI  (2438)
1 site		 A T T A A T T A A T