pSF-CMV-FMDV-RLuc

Mammalian vector for co-expressing a gene together with Renilla luciferase expressed from the foot-and-mouth disease virus IRES.

Sequence Author: Oxford Genetics

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PmeI (5531) RsrII (5224) BsrDI (4941) PflFI - Tth111I (4826) PmeI (4483) SanDI (4417) AscI (4326) FseI (4180) NaeI (4178) NgoMIV (4176) SwaI (4074) BspHI (3905) AlwNI (3601) BssSI (3358) SwaI (3181) PacI (3055) 3' β-globin insulator PstI - SbfI (2923) AsiSI - PvuI (5) BglII (232) CMV enhancer SnaBI (566) BglII (816) NotI (858) Eco53kI (879) SacI (881) EcoRI (885) NcoI (905) EcoRV (921) AbsI - PaeR7I - PspXI - XhoI (928) BsgI (940) BspDI - ClaI (977) EcoNI (1011) PspOMI (1226) ApaI (1230) PflMI (1308) AleI (1309) AhdI (1438) BstAPI (1661) AanI (1765) NheI (2407) BmtI (2411) stop codons AanI (2557) HpaI (2577) MfeI (2586) pSF-CMV-FMDV-RLuc 5650 bp
PmeI  (5531)
2 sites
G T T T A A A C C A A A T T T G
RsrII  (5224)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (4941)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (4826)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4826)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PmeI  (4483)
2 sites
G T T T A A A C C A A A T T T G
SanDI  (4417)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
AscI  (4326)
1 site
G G C G C G C C C C G C G C G G
FseI  (4180)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
NaeI  (4178)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (4176)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SwaI  (4074)
2 sites
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
BspHI  (3905)
1 site
T C A T G A A G T A C T
AlwNI  (3601)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BssSI  (3358)
1 site
C A C G A G G T G C T C
SwaI  (3181)
2 sites
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
PacI  (3055)
1 site
T T A A T T A A A A T T A A T T
PstI  (2923)
1 site
C T G C A G G A C G T C
SbfI  (2923)
1 site
C C T G C A G G G G A C G T C C
AsiSI  (5)
1 site
G C G A T C G C C G C T A G C G
PvuI  (5)
1 site
C G A T C G G C T A G C
BglII  (232)
2 sites
A G A T C T T C T A G A
SnaBI  (566)
1 site
T A C G T A A T G C A T
BglII  (816)
2 sites
A G A T C T T C T A G A
NotI  (858)
1 site
G C G G C C G C C G C C G G C G
Eco53kI  (879)
1 site
G A G C T C C T C G A G
SacI  (881)
1 site
G A G C T C C T C G A G
EcoRI  (885)
1 site
G A A T T C C T T A A G
NcoI  (905)
1 site
C C A T G G G G T A C C
EcoRV  (921)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
AbsI  (928)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (928)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (928)
1 site
V C T C G A G B B G A G C T C V
XhoI  (928)
1 site
C T C G A G G A G C T C
BsgI  (940)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BspDI  (977)
1 site
A T C G A T T A G C T A
ClaI  (977)
1 site
A T C G A T T A G C T A
EcoNI  (1011)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PspOMI  (1226)
1 site
G G G C C C C C C G G G
ApaI  (1230)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PflMI  (1308)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AleI  (1309)
1 site
C A C N N N N G T G G T G N N N N C A C
AhdI  (1438)
1 site