Fluorescent Timer

Green-to-red fluorescent timer derivative of DsRed.

Sequence Author: Clontech (TaKaRa)

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No matches
600 400 200 End (681) AleI (631) BsgI (583) PvuII - MspA1I (567) MscI (550) BstXI (546) CsiI - SexAI * (521) EcoO109I (492) MflI * - BstYI (482) HaeII (450) StuI (436) PspFI (432) BseYI (428) BbsI (424) BtgI - NcoI (422) MmeI (419) PstI - SbfI (344) BseRI (324) BsiEI (320) AvaI - BsoBI (265) BmrI (186) AhdI (183) BstEII (144) BssSI - BssSαI (76) BaeGI - Bsp1286I - Bme1580I (64) BanI (61) FspI - FspAI (52) Start (0) Timer Fluorescent Timer 681 bp
End  (681)
0 sites
AleI  (631)
1 site
C A C N N N N G T G G T G N N N N C A C
BsgI  (583)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PvuII  (567)
1 site
C A G C T G G T C G A C
MspA1I  (567)
1 site
C M G C K G G K C G M C
MscI  (550)
1 site
T G G C C A A C C G G T
BstXI  (546)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
CsiI  (521)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (521)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
EcoO109I  (492)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
MflI  (482)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (482)
1 site
R G A T C Y Y C T A G R
HaeII  (450)
1 site
R G C G C Y Y C G C G R
StuI  (436)
1 site
A G G C C T T C C G G A
PspFI  (432)
1 site
C C C A G C G G G T C G
BseYI  (428)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BbsI  (424)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BtgI  (422)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (422)
1 site
C C A T G G G G T A C C
MmeI  (419)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PstI  (344)
1 site
C T G C A G G A C G T C
SbfI  (344)
1 site
C C T G C A G G G G A C G T C C
BseRI  (324)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BsiEI  (320)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
AvaI  (265)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (265)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
BmrI  (186)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
AhdI  (183)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BstEII  (144)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BssSI  (76)
1 site
C A C G A G G T G C T C
BssSαI  (76)
1 site
C A C G A G G T G C T C
BaeGI  (64)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bsp1286I  (64)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
Bme1580I  (64)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BanI  (61)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
FspI  (52)
1 site
T G C G C A A C G C G T
FspAI  (52)
1 site
R T G C G C A Y Y A C G C G T R
Start  (0)
0 sites
Timer
1 .. 681  =  681 bp
226 amino acids  =  26.0 kDa
3 segments
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: green-to-red fluorescent timer derivative of DsRed (Terskikh et al., 2000)
mammalian codon-optimized
Timer
1 .. 681  =  681 bp
226 amino acids  =  26.0 kDa
3 segments
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.1 Da
Product: green-to-red fluorescent timer derivative of DsRed (Terskikh et al., 2000)
mammalian codon-optimized
Timer
1 .. 681  =  681 bp
226 amino acids  =  26.0 kDa
3 segments
   Segment 3:  
   7 .. 681  =  675 bp
   224 amino acids  =  25.8 kDa
Product: green-to-red fluorescent timer derivative of DsRed (Terskikh et al., 2000)
mammalian codon-optimized
Timer
1 .. 681  =  681 bp
226 amino acids  =  26.0 kDa
3 segments
Product: green-to-red fluorescent timer derivative of DsRed (Terskikh et al., 2000)
mammalian codon-optimized
ORF:  1 .. 681  =  681 bp
ORF:  226 amino acids  =  26.0 kDa
ORF:  3 .. 680  =  678 bp
ORF:  226 amino acids  =  25.0 kDa  (no start codon)
ORF:  1 .. 681  =  681 bp
ORF:  227 amino acids  =  22.7 kDa  (no start codon)
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