GFP

Aequorea victoria green fluorescent protein.

Sequence Author: Clontech (TaKaRa)

|Download SnapGene Viewer
No matches
600 400 200 End (717) PspFI (683) BpuEI (682) BseYI - AlwNI (679) PvuII - MspA1I (676) SmlI (661) DrdI (652) EcoP15I (649) BsaI (638) MflI * - BstYI (627) BstBI (622) BtgZI (581) MfeI (560) HpaI - HincII (489) BstZ17I (450) AccI (449) DraI (390) AcuI (350) PmlI BsaAI (325) AflIII (322) TatI - BsrGI (276) NdeI (230) MscI (171) EaeI (169) BtgI - StyI - NcoI (166) BaeGI - Bsp1286I - Bme1580I (74) BpmI (47) Start (0) GFP GFP 717 bp
End  (717)
0 sites
PspFI  (683)
1 site
C C C A G C G G G T C G
BpuEI  (682)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BseYI  (679)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI  (679)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PvuII  (676)
1 site
C A G C T G G T C G A C
MspA1I  (676)
1 site
C M G C K G G K C G M C
SmlI  (661)
1 site
C T Y R A G G A R Y T C

Cleavage may be enhanced when more than one copy of the SmlI recognition sequence is present.
Sticky ends from different SmlI sites may not be compatible.
DrdI  (652)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
EcoP15I  (649)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BsaI  (638)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
MflI  (627)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (627)
1 site
R G A T C Y Y C T A G R
BstBI  (622)
1 site
T T C G A A A A G C T T
BtgZI  (581)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
MfeI  (560)
1 site
C A A T T G G T T A A C
HpaI  (489)
1 site
G T T A A C C A A T T G
HincII  (489)
1 site
G T Y R A C C A R Y T G
BstZ17I  (450)
1 site
G T A T A C C A T A T G
AccI  (449)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
DraI  (390)
1 site
T T T A A A A A A T T T
AcuI  (350)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PmlI  (325)
1 site
C A C G T G G T G C A C
BsaAI  (325)
1 site
Y A C G T R R T G C A Y
AflIII  (322)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
TatI  (276)
1 site
W G T A C W W C A T G W
BsrGI  (276)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
NdeI  (230)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
MscI  (171)
1 site
T G G C C A A C C G G T
EaeI  (169)
1 site
Y G G C C R R C C G G Y
BtgI  (166)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
StyI  (166)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
NcoI  (166)
1 site
C C A T G G G G T A C C
BaeGI  (74)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bsp1286I  (74)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
Bme1580I  (74)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BpmI  (47)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Start  (0)
0 sites
GFP
1 .. 717  =  717 bp
238 amino acids  =  26.9 kDa
Product: Aequoria victoria green fluorescent protein
GFP
1 .. 717  =  717 bp
238 amino acids  =  26.9 kDa
Product: Aequoria victoria green fluorescent protein
ORF:  1 .. 717  =  717 bp
ORF:  238 amino acids  =  26.9 kDa
Click here to try SnapGene

Download GFP.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.