PAmKate

Monomeric photoactivatable red fluorescent protein derived from mKate.

Sequence Author: Verkhusha Lab

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No matches
600 400 200 End (714) PacI (711) BmrI - HindIII (703) AlwNI (674) MscI (667) EaeI (665) DraIII (657) PshAI (640) BsaI (628) AccI (587) BsaHI (584) SmlI (571) BpuEI (556) MflI * BstYI (547) AcuI (517) BglI (476) MspA1I (460) PspFI (432) BseYI (428) MmeI (419) NmeAIII (405) PfoI * (341) BpmI (325) Bsu36I (266) PasI (231) TsoI (175) BsiHKAI (91) ApaLI (87) TatI - BsrGI (50) NspI (39) EarI - SapI - BspQI (11) Start (0) PAmKate PAmKate 714 bp
End  (714)
0 sites
PacI  (711)
1 site
T T A A T T A A A A T T A A T T
BmrI  (703)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
HindIII  (703)
1 site
A A G C T T T T C G A A
AlwNI  (674)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
MscI  (667)
1 site
T G G C C A A C C G G T
EaeI  (665)
1 site
Y G G C C R R C C G G Y
DraIII  (657)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PshAI  (640)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BsaI  (628)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AccI  (587)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BsaHI  (584)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
SmlI  (571)
1 site
C T Y R A G G A R Y T C

Cleavage may be enhanced when more than one copy of the SmlI recognition sequence is present.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (556)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MflI  (547)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (547)
1 site
R G A T C Y Y C T A G R
AcuI  (517)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (476)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
MspA1I  (460)
1 site
C M G C K G G K C G M C
PspFI  (432)
1 site
C C C A G C G G G T C G
BseYI  (428)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
MmeI  (419)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NmeAIII  (405)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PfoI  (341)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BpmI  (325)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Bsu36I  (266)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PasI  (231)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
TsoI  (175)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsiHKAI  (91)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (87)
1 site
G T G C A C C A C G T G
TatI  (50)
1 site
W G T A C W W C A T G W
BsrGI  (50)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
NspI  (39)
1 site
R C A T G Y Y G T A C R
EarI  (11)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
SapI  (11)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BspQI  (11)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
Start  (0)
0 sites
PAmKate
1 .. 714  =  714 bp
237 amino acids  =  26.7 kDa
Product: monomeric photoactivatable red fluorescent protein (Gunewardene et al., 2011)
mammalian codon-optimized
PAmKate
1 .. 714  =  714 bp
237 amino acids  =  26.7 kDa
Product: monomeric photoactivatable red fluorescent protein (Gunewardene et al., 2011)
mammalian codon-optimized
ORF:  1 .. 714  =  714 bp
ORF:  237 amino acids  =  26.7 kDa
ORF:  1 .. 714  =  714 bp
ORF:  238 amino acids  =  24.7 kDa  (no start codon)
ORF:  114 .. 359  =  246 bp
ORF:  81 amino acids  =  9.1 kDa
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Download PAmKate.dna file

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