Y-FAST

Small protein tag, derived from photoactive yellow protein, that allows in vivo fluorescent labeling by addition of a cell-permeant fluorogen.
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No matches
300 200 100 End (375) BsaWI (340) BaeGI - BsiHKAI - Bsp1286I - Bme1580I (323) ApaLI (319) StyI (308) BstBI (275) ApoI (232) AvaI - BsoBI (217) MflI * - BstYI (156) AcuI - Eco57MI (155) PstI - TatI (122) SfcI (118) EcoP15I (103) MscI (46) EaeI (44) BtsI - BtsαI (27) NspI (10) Start (0) Y-FAST Y-FAST 375 bp
End  (375)
0 sites
BsaWI  (340)
1 site
W C C G G W W G G C C W

Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
BaeGI  (323)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
BsiHKAI  (323)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
Bsp1286I  (323)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
Bme1580I  (323)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
ApaLI  (319)
1 site
G T G C A C C A C G T G
StyI  (308)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BstBI  (275)
1 site
T T C G A A A A G C T T
ApoI  (232)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
AvaI  (217)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (217)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
MflI  (156)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (156)
1 site
R G A T C Y Y C T A G R
AcuI  (155)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (155)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PstI  (122)
1 site
C T G C A G G A C G T C
TatI  (122)
1 site
W G T A C W W C A T G W
SfcI  (118)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
EcoP15I  (103)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
MscI  (46)
1 site
T G G C C A A C C G G T
EaeI  (44)
1 site
Y G G C C R R C C G G Y
BtsI  (27)
1 site
G C A G T G N N C G T C A C
BtsαI  (27)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
NspI  (10)
1 site
R C A T G Y Y G T A C R
Start  (0)
0 sites
Y-FAST
1 .. 375  =  375 bp
125 amino acids  =  13.7 kDa
Product: small protein tag, derived from photoactive yellow protein, that allows in vivo fluorescent labeling by addition of a cell-permeant fluorogen (Plamont et al., 2016)
Y-FAST
1 .. 375  =  375 bp
125 amino acids  =  13.7 kDa
Product: small protein tag, derived from photoactive yellow protein, that allows in vivo fluorescent labeling by addition of a cell-permeant fluorogen (Plamont et al., 2016)
ORF:  1 .. 375  =  375 bp
ORF:  125 amino acids  =  13.7 kDa
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SnapGene

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Individual Sequences & Maps

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