mCarmine

Monomeric far-red fluorescent protein with favorable expression and pKa properties and with peak emission at 675 nm.
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No matches
600 400 200 End (735) BmrI (703) AlwNI (674) MscI (667) EaeI (665) DraIII (657) PshAI (640) AccI (587) BsaHI (584) MflI * - BstYI (547) BfuAI PaqCI BspMI (527) PstI (524) SfcI (520) BglI (476) MspA1I (460) PspFI (432) BseYI (428) MmeI (419) NmeAIII (405) PfoI * (341) BpmI (325) BbsI (308) Bsu36I (266) PasI (231) TsoI (175) BsiHKAI (91) ApaLI (87) MslI (40) NspI (39) EarI - SapI - BspQI (11) Start (0) mCarmine mCarmine 735 bp
End  (735)
0 sites
BmrI  (703)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
AlwNI  (674)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
MscI  (667)
1 site
T G G C C A A C C G G T
EaeI  (665)
1 site
Y G G C C R R C C G G Y
DraIII  (657)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PshAI  (640)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AccI  (587)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BsaHI  (584)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
MflI  (547)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (547)
1 site
R G A T C Y Y C T A G R
BfuAI  (527)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
PaqCI  (527)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
BspMI  (527)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PstI  (524)
1 site
C T G C A G G A C G T C
SfcI  (520)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BglI  (476)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
MspA1I  (460)
1 site
C M G C K G G K C G M C
PspFI  (432)
1 site
C C C A G C G G G T C G
BseYI  (428)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
MmeI  (419)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NmeAIII  (405)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PfoI  (341)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BpmI  (325)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BbsI  (308)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
Bsu36I  (266)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PasI  (231)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
TsoI  (175)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsiHKAI  (91)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (87)
1 site
G T G C A C C A C G T G
MslI  (40)
1 site
C A Y N N N N R T G G T R N N N N Y A C
NspI  (39)
1 site
R C A T G Y Y G T A C R
EarI  (11)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
SapI  (11)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BspQI  (11)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
Start  (0)
0 sites
mCarmine
1 .. 735  =  735 bp
244 amino acids  =  27.7 kDa
Product: monomeric far-red fluorescent protein with favorable expression and pKa properties and with peak emission at 675 nm (Fabritius et al., 2018)
mCarmine
1 .. 735  =  735 bp
244 amino acids  =  27.7 kDa
Product: monomeric far-red fluorescent protein with favorable expression and pKa properties and with peak emission at 675 nm (Fabritius et al., 2018)
ORF:  1 .. 735  =  735 bp
ORF:  244 amino acids  =  27.7 kDa
ORF:  1 .. 735  =  735 bp
ORF:  245 amino acids  =  25.7 kDa  (no start codon)
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Download mCarmine.dna file

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