mCherry2C

mCherry derivative in which the N- and C-termini have been modified to reduce cytotoxicity.
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No matches
600 400 200 End (711) MseI (708) NmeAIII (689) PasI (687) BglI (680) BccI (649) AleI - MslI (643) HincII (600) BsgI (595) NciI (584) PvuII - MspA1I (579) HgaI (544) DrdI (541) BbvCI - Bpu10I (539) AlwNI (507) PspFI (444) BseYI (440) BbsI (436) PstI - SbfI (356) SfcI (352) FauI (249) BsaAI (231) AhdI (195) BssSI - BssSαI (88) BanII (76) BsiHKAI (65) ApaLI (61) TspRI (15) BtsIMutI (8) Start (0) mCherry2C mCherry2C 711 bp
End  (711)
0 sites
MseI  (708)
1 site
T T A A A A T T
NmeAIII  (689)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PasI  (687)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BglI  (680)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BccI  (649)
1 site
C C A T C ( N ) 4 G G T A G ( N ) 4 N

The 1-base overhangs produced by BccI may be hard to ligate.
Sticky ends from different BccI sites may not be compatible.
AleI  (643)
1 site
C A C N N N N G T G G T G N N N N C A C
MslI  (643)
1 site
C A Y N N N N R T G G T R N N N N Y A C
HincII  (600)
1 site
G T Y R A C C A R Y T G
BsgI  (595)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NciI  (584)
1 site
C C S G G G G S C C

The 1-base overhangs produced by NciI may be hard to ligate.
Sticky ends from different NciI sites may not be compatible.
PvuII  (579)
1 site
C A G C T G G T C G A C
MspA1I  (579)
1 site
C M G C K G G K C G M C
HgaI  (544)
1 site
G A C G C ( N ) 5 C T G C G ( N ) 5 ( N ) 5

Cleavage may be enhanced when more than one copy of the HgaI recognition sequence is present.
Sticky ends from different HgaI sites may not be compatible.
DrdI  (541)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BbvCI  (539)
1 site
C C T C A G C G G A G T C G
Bpu10I  (539)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AlwNI  (507)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (444)
1 site
C C C A G C G G G T C G
BseYI  (440)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BbsI  (436)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PstI  (356)
1 site
C T G C A G G A C G T C
SbfI  (356)
1 site
C C T G C A G G G G A C G T C C
SfcI  (352)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
FauI  (249)
1 site
C C C G C ( N ) 4 G G G C G ( N ) 4 N N

Sticky ends from different FauI sites may not be compatible.
BsaAI  (231)
1 site
Y A C G T R R T G C A Y
AhdI  (195)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BssSI  (88)
1 site
C A C G A G G T G C T C
BssSαI  (88)
1 site
C A C G A G G T G C T C
BanII  (76)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BsiHKAI  (65)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (61)
1 site
G T G C A C C A C G T G
TspRI  (15)
1 site
N N C A S T G N N N N G T S A C N N

Sticky ends from different TspRI sites may not be compatible.
After cleavage, FastDigest® TspRI can remain bound to DNA and alter its electrophoretic mobility.
TspRI generates a 9-base extension that can alter the electrophoretic mobility of DNA fragments.
BtsIMutI  (8)
1 site
C A G T G N N G T C A C
Start  (0)
0 sites
mCherry2C
1 .. 711  =  711 bp
236 amino acids  =  26.3 kDa
Product: mCherry derivative in which the N- and C-termini have been modified to reduce cytotoxicity (Valbuena et al., 2020)
preserves the original mammalian codon optimization
mCherry2C
1 .. 711  =  711 bp
236 amino acids  =  26.3 kDa
Product: mCherry derivative in which the N- and C-termini have been modified to reduce cytotoxicity (Valbuena et al., 2020)
preserves the original mammalian codon optimization
ORF:  1 .. 711  =  711 bp
ORF:  236 amino acids  =  26.3 kDa
ORF:  1 .. 711  =  711 bp
ORF:  237 amino acids  =  23.2 kDa  (no start codon)
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Download mCherry2C.dna file

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Individual Sequences & Maps

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