mGold

Photostable derivative of the yellow fluorescent protein mVenus.
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No matches
600 400 200 End (720) BsrGI (710) BlpI (622) Bpu10I (604) MflI * - BstYI (500) PfoI * (281) BtsI - BtsαI (214) PstI (209) SfcI (205) BssSI - BssSαI (181) BsrFI (151) BtgZI (122) EaeI (72) BanI (36) BseRI (31) Start (0) mGold mGold 720 bp
End  (720)
0 sites
BsrGI  (710)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BlpI  (622)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
Bpu10I  (604)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
MflI  (500)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (500)
1 site
R G A T C Y Y C T A G R
PfoI  (281)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BtsI  (214)
1 site
G C A G T G N N C G T C A C
BtsαI  (214)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
PstI  (209)
1 site
C T G C A G G A C G T C
SfcI  (205)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BssSI  (181)
1 site
C A C G A G G T G C T C
BssSαI  (181)
1 site
C A C G A G G T G C T C
BsrFI  (151)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI  (122)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
EaeI  (72)
1 site
Y G G C C R R C C G G Y
BanI  (36)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
Start  (0)
0 sites
mGold
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: photostable derivative of mVenus (Lee et al., 2020)
mammalian codon-optimized
mGold
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.1 Da
Product: photostable derivative of mVenus (Lee et al., 2020)
mammalian codon-optimized
mGold
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 3:  
   7 .. 720  =  714 bp
   237 amino acids  =  26.7 kDa
Product: photostable derivative of mVenus (Lee et al., 2020)
mammalian codon-optimized
mGold
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
Product: photostable derivative of mVenus (Lee et al., 2020)
mammalian codon-optimized
ORF:  1 .. 720  =  720 bp
ORF:  239 amino acids  =  26.9 kDa
ORF:  3 .. 719  =  717 bp
ORF:  239 amino acids  =  27.6 kDa  (no start codon)
ORF:  1 .. 720  =  720 bp
ORF:  240 amino acids  =  24.0 kDa  (no start codon)
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Download mGold.dna file

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Individual Sequences & Maps

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