mGreenLantern

Monomeric GFP derivative that displays exceptionally high brightness in cells.
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No matches
600 400 200 End (720) BsrGI (710) BstEII (672) BlpI (622) Bpu10I (604) AclI (512) BsiEI (464) EagI (461) BpmI (445) NmeAIII (356) KpnI (317) Acc65I (313) PfoI * (281) EciI (249) Bsu36I (192) BssSI - BssSαI (181) BsrFI (151) BtgZI (122) SacII (93) MspA1I (92) BtgI (90) EGFP-N (46 .. 67) BseRI (31) Start (0) mGreenLantern mGreenLantern 720 bp
End  (720)
0 sites
BsrGI  (710)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BstEII  (672)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BlpI  (622)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
Bpu10I  (604)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AclI  (512)
1 site
A A C G T T T T G C A A
BsiEI  (464)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
EagI  (461)
1 site
C G G C C G G C C G G C
BpmI  (445)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
NmeAIII  (356)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
KpnI  (317)
1 site
G G T A C C C C A T G G
Acc65I  (313)
1 site
G G T A C C C C A T G G
PfoI  (281)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
EciI  (249)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
Bsu36I  (192)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BssSI  (181)
1 site
C A C G A G G T G C T C
BssSαI  (181)
1 site
C A C G A G G T G C T C
BsrFI  (151)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI  (122)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
SacII  (93)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
MspA1I  (92)
1 site
C M G C K G G K C G M C
BtgI  (90)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
Start  (0)
0 sites
EGFP-N
22-mer  /  73% GC
1 binding site
46 .. 67  =  22 annealed bases
Tm  =  67°C
EGFP, reverse primer
mGreenLantern
1 .. 720  =  720 bp
239 amino acids  =  26.8 kDa
3 segments
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: monomeric GFP derivative that displays exceptionally high brightness in cells (Campbell et al., 2020)
mGreenLantern
1 .. 720  =  720 bp
239 amino acids  =  26.8 kDa
3 segments
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.1 Da
Product: monomeric GFP derivative that displays exceptionally high brightness in cells (Campbell et al., 2020)
mGreenLantern
1 .. 720  =  720 bp
239 amino acids  =  26.8 kDa
3 segments
   Segment 3:  
   7 .. 720  =  714 bp
   237 amino acids  =  26.6 kDa
Product: monomeric GFP derivative that displays exceptionally high brightness in cells (Campbell et al., 2020)
mGreenLantern
1 .. 720  =  720 bp
239 amino acids  =  26.8 kDa
3 segments
Product: monomeric GFP derivative that displays exceptionally high brightness in cells (Campbell et al., 2020)
ORF:  1 .. 720  =  720 bp
ORF:  239 amino acids  =  26.8 kDa
ORF:  3 .. 494  =  492 bp
ORF:  163 amino acids  =  18.8 kDa  (no start codon)
ORF:  193 .. 720  =  528 bp
ORF:  175 amino acids  =  17.7 kDa  (no start codon)
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Download mGreenLantern.dna file

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