mIFP

Naturally monomeric infrared fluorescent protein derived from a truncated bacteriophytochrome.
|Download SnapGene Viewer
No matches
800 600 400 200 End (960) BpmI - Eco57MI (952) NruI (925) BsaBI * (919) BtgZI (906) HaeII (898) AfeI (896) NspI (768) SfcI (676) BsaWI (666) MslI (632) AccI (592) BsaHI (589) BstEII (574) ApoI (530) EarI (509) NaeI (389) BsrFI - NgoMIV (387) BsrDI (380) BtgI (284) BsgI (238) AlwNI - BstAPI (231) BciVI (109) BanII - Bsp1286I (82) MluI - AflIII (35) BsmI (25) BbvCI - Bpu10I (21) KpnI (10) BanI - Acc65I (6) Start (0) mIFP mIFP 960 bp
End  (960)
0 sites
BpmI  (952)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Eco57MI  (952)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
NruI  (925)
1 site
T C G C G A A G C G C T
BsaBI  (919)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BtgZI  (906)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
HaeII  (898)
1 site
R G C G C Y Y C G C G R
AfeI  (896)
1 site
A G C G C T T C G C G A
NspI  (768)
1 site
R C A T G Y Y G T A C R
SfcI  (676)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BsaWI  (666)
1 site
W C C G G W W G G C C W

Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
MslI  (632)
1 site
C A Y N N N N R T G G T R N N N N Y A C
AccI  (592)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BsaHI  (589)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
BstEII  (574)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
ApoI  (530)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EarI  (509)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
NaeI  (389)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
BsrFI  (387)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
NgoMIV  (387)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BsrDI  (380)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BtgI  (284)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BsgI  (238)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (231)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BstAPI  (231)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BciVI  (109)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BanII  (82)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Bsp1286I  (82)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
MluI  (35)
1 site
A C G C G T T G C G C A
AflIII  (35)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
BsmI  (25)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BbvCI  (21)
1 site
C C T C A G C G G A G T C G
Bpu10I  (21)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
KpnI  (10)
1 site
G G T A C C C C A T G G
BanI  (6)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
Acc65I  (6)
1 site
G G T A C C C C A T G G
Start  (0)
0 sites
mIFP
1 .. 960  =  960 bp
320 amino acids  =  35.1 kDa
Product: naturally monomeric infrared fluorescent protein derived from a truncated bacteriophytochrome (Yu et al., 2015)
mIFP
1 .. 960  =  960 bp
320 amino acids  =  35.1 kDa
Product: naturally monomeric infrared fluorescent protein derived from a truncated bacteriophytochrome (Yu et al., 2015)
ORF:  1 .. 960  =  960 bp
ORF:  320 amino acids  =  35.1 kDa
ORF:  22 .. 411  =  390 bp
ORF:  129 amino acids  =  14.9 kDa
ORF:  676 .. 960  =  285 bp
ORF:  94 amino acids  =  10.8 kDa  (no start codon)
ORF:  87 .. 320  =  234 bp
ORF:  77 amino acids  =  8.4 kDa
Click here to try SnapGene

Download mIFP.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.