miniSOG

Mini singlet oxygen generator, a fluorescent flavoprotein suitable for correlated light and electron microscopy.

Sequence Author: Tsien Lab

|Download SnapGene Viewer
No matches
300 200 100 End (321) MslI (301) ScaI (292) BstAPI (264) PaqCI (263) BsrBI (227) BsaHI (173) AvaI - ApaI - BanII - BaeGI - BsoBI - Bsp1286I - Bme1580I (136) BsaI (133) PspOMI (132) SbfI (131) BsiEI (120) EaeI - EagI (117) SfiI - BglI (116) BseRI (112) BpmI - Eco57MI * (94) PfoI * (71) XmnI (10) EarI - SapI - BspQI (1) Start (0) miniSOG miniSOG 321 bp
End  (321)
0 sites
MslI  (301)
1 site
C A Y N N N N R T G G T R N N N N Y A C
ScaI  (292)
1 site
A G T A C T T C A T G A
BstAPI  (264)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
PaqCI  (263)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
BsrBI  (227)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
BsaHI  (173)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
AvaI  (136)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
ApaI  (136)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (136)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BaeGI  (136)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
BsoBI  (136)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
Bsp1286I  (136)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
Bme1580I  (136)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BsaI  (133)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PspOMI  (132)
1 site
G G G C C C C C C G G G
SbfI  (131)
1 site
C C T G C A G G G G A C G T C C
BsiEI  (120)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
EaeI  (117)
1 site
Y G G C C R R C C G G Y
EagI  (117)
1 site
C G G C C G G C C G G C
SfiI  (116)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BglI  (116)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BseRI  (112)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BpmI  (94)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Eco57MI  (94)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14
* Blocked by Dcm methylation.
Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PfoI  (71)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
XmnI  (10)
1 site
G A A N N N N T T C C T T N N N N A A G
EarI  (1)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
SapI  (1)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BspQI  (1)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
Start  (0)
0 sites
miniSOG
1 .. 321  =  321 bp
106 amino acids  =  12.3 kDa
Product: mini singlet oxygen generator, a fluorescent flavoprotein suitable for correlated light and electron microscopy (Shu et al., 2011)
miniSOG
1 .. 321  =  321 bp
106 amino acids  =  12.3 kDa
Product: mini singlet oxygen generator, a fluorescent flavoprotein suitable for correlated light and electron microscopy (Shu et al., 2011)
ORF:  1 .. 321  =  321 bp
ORF:  106 amino acids  =  12.3 kDa
ORF:  3 .. 320  =  318 bp
ORF:  106 amino acids  =  12.0 kDa  (no start codon)
ORF:  1 .. 321  =  321 bp
ORF:  107 amino acids  =  10.7 kDa  (no start codon)
Click here to try SnapGene

Download miniSOG.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.