pFusionRed-B

Vector for expressing the monomeric red fluorescent protein FusionRed in bacteria.

Sequence Author: Evrogen

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No matches
EcoO109I (4113) AatII (4059) ZraI (4057) XmnI (3736) PvuI (3507) FspI (3359) AseI (3309) AhdI (3137) AlwNI (2660) AflIII - PciI (2244) BspQI - SapI (2128) BstAPI (2068) NdeI (2067) AvaI - BsoBI - PaeR7I - XhoI (1) T5 promoter PsiI (49) MfeI (59) lac operator BseRI (114) BsaBI * (125) BamHI (126) AgeI (134) AleI (147) BsrGI (187) Bsu36I (403) StuI (573) PshAI (777) DraIII (794) EagI - NotI (847) HindIII (854) BlpI (866) NheI (974) BmtI (978) Bpu10I (999) PvuII (1126) BspEI (1222) PasI (1458) XbaI (1831) PflFI - Tth111I (1991) BsaAI (1998) BstZ17I (2017) pFusionRed-B 4129 bp
EcoO109I  (4113)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (4059)
1 site
G A C G T C C T G C A G
ZraI  (4057)
1 site
G A C G T C C T G C A G
XmnI  (3736)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (3507)
1 site
C G A T C G G C T A G C
FspI  (3359)
1 site
T G C G C A A C G C G T
AseI  (3309)
1 site
A T T A A T T A A T T A
AhdI  (3137)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2660)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AflIII  (2244)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2244)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2128)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2128)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstAPI  (2068)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
NdeI  (2067)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
AvaI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T G G T T A A C
BseRI  (114)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BsaBI  (125)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BamHI  (126)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AgeI  (134)
1 site
A C C G G T T G G C C A
AleI  (147)
1 site
C A C N N N N G T G G T G N N N N C A C
BsrGI  (187)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
Bsu36I  (403)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
StuI  (573)
1 site
A G G C C T T C C G G A
PshAI  (777)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
DraIII  (794)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
EagI  (847)
1 site
C G G C C G G C C G G C
NotI  (847)
1 site
G C G G C C G C C G C C G G C G
HindIII  (854)
1 site
A A G C T T T T C G A A
BlpI  (866)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
NheI  (974)
1 site
G C T A G C C G A T C G
BmtI  (978)
1 site
G C T A G C C G A T C G
Bpu10I  (999)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PvuII  (1126)
1 site
C A G C T G G T C G A C
BspEI  (1222)
1 site
T C C G G A A G G C C T
PasI  (1458)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
XbaI  (1831)
1 site
T C T A G A A G A T C T
PflFI  (1991)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1991)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsaAI  (1998)
1 site
Y A C G T R R T G C A Y
BstZ17I  (2017)
1 site
G T A T A C C A T A T G
AmpR
3064 .. 3924  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   3064 .. 3855  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3064 .. 3924  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   3856 .. 3924  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3064 .. 3924  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ATG
114 .. 116  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
114 .. 116  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
FusionRed
147 .. 845  =  699 bp
232 amino acids  =  25.6 kDa
Product: monomeric red fluorescent protein optimized for use as a fusion tag (Shemiakina et al., 2012)
mammalian codon-optimized
FusionRed
147 .. 845  =  699 bp
232 amino acids  =  25.6 kDa
Product: monomeric red fluorescent protein optimized for use as a fusion tag (Shemiakina et al., 2012)
mammalian codon-optimized
CmR
1013 .. 1672  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
1013 .. 1672  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
2305 .. 2893  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2305 .. 2893  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
AmpR promoter
3925 .. 4029  =  105 bp
AmpR promoter
3925 .. 4029  =  105 bp
lambda t0 terminator
875 .. 969  =  95 bp
transcription terminator from phage lambda
lambda t0 terminator
875 .. 969  =  95 bp
transcription terminator from phage lambda
rrnB T1 terminator
1737 .. 1823  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
1737 .. 1823  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
T5 promoter
10 .. 54  =  45 bp
4 segments
   Segment 1:  
   10 .. 24  =  15 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
4 segments
   Segment 2:  -35  
   25 .. 30  =  6 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
4 segments
   Segment 3:  
   31 .. 47  =  17 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
4 segments
   Segment 4:  -10  
   48 .. 54  =  7 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
4 segments
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
lac operator
62 .. 78  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
62 .. 78  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
30 .. 46  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
30 .. 46  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
ORF:  1013 .. 1672  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  3194 .. 3460  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  114 .. 845  =  732 bp
ORF:  243 amino acids  =  26.6 kDa
ORF:  251 .. 496  =  246 bp
ORF:  81 amino acids  =  8.8 kDa
ORF:  3064 .. 3924  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
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