pMFAPalpha2

Vector expressing a plasma membrane-localized version of the fluorogen-activating protein FAPα2.

Sequence Author: SpectraGenetics

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PsiI (5950) DraIII (5825) XmnI (5272) ScaI (5153) PvuI (5043) AhdI (4673) SexAI * (4425) StuI (4195) BspDI * - ClaI * (4174) BsaBI * (4157) PluTI (4017) SfoI (4015) NarI (4014) KasI (4013) BclI * (3673) RsrII (3503) BspQI - SapI (3465) BstBI (3338) BssHII - MauBI (1) CMV enhancer SnaBI (365) HindIII (664) Acc65I (670) KpnI (674) BstXI (709) EcoRI (713) EcoRV (729) HA PspOMI (828) ApaI (832) SfiI (836) BlpI (1080) BmgBI (1123) BspEI (1214) SfiI (1569) SacII (1583) PstI (1590) SalI (1592) AccI (1593) BsmI (1633) NotI (1782) AvaI - BmeT110I - BsoBI - PaeR7I - XhoI (1788) BbsI (1994) AflIII - PciI (2100) pMFAP α2 6049 bp
PsiI  (5950)
1 site
T T A T A A A A T A T T
DraIII  (5825)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
XmnI  (5272)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (5153)
1 site
A G T A C T T C A T G A
PvuI  (5043)
1 site
C G A T C G G C T A G C
AhdI  (4673)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SexAI  (4425)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
StuI  (4195)
1 site
A G G C C T T C C G G A
BspDI  (4174)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (4174)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
BsaBI  (4157)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
PluTI  (4017)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (4015)
1 site
G G C G C C C C G C G G
NarI  (4014)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (4013)
1 site
G G C G C C C C G C G G
BclI  (3673)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
RsrII  (3503)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BspQI  (3465)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3465)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstBI  (3338)
1 site
T T C G A A A A G C T T
BssHII  (1)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
MauBI  (1)
1 site
C G C G C G C G G C G C G C G C
SnaBI  (365)
1 site
T A C G T A A T G C A T
HindIII  (664)
1 site
A A G C T T T T C G A A
Acc65I  (670)
1 site
G G T A C C C C A T G G
KpnI  (674)
1 site
G G T A C C C C A T G G
BstXI  (709)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoRI  (713)
1 site
G A A T T C C T T A A G
EcoRV  (729)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PspOMI  (828)
1 site
G G G C C C C C C G G G
ApaI  (832)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SfiI  (836)
2 sites
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BlpI  (1080)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BmgBI  (1123)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BspEI  (1214)
1 site
T C C G G A A G G C C T
SfiI  (1569)
2 sites
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
SacII  (1583)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PstI  (1590)
1 site
C T G C A G G A C G T C
SalI  (1592)
1 site
G T C G A C C A G C T G
AccI  (1593)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BsmI  (1633)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
NotI  (1782)
1 site
G C G G C C G C C G C C G G C G
AvaI  (1788)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BmeT110I  (1788)
1 site
C Y C G R G G R G C Y C
BsoBI  (1788)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (1788)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1788)
1 site
C T C G A G G A G C T C
BbsI  (1994)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
AflIII  (2100)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2100)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
Igκ leader
737 .. 799  =  63 bp
21 amino acids  =  2.4 kDa
Product: leader sequence from mouse immunoglobulin κ light chain
Igκ leader
737 .. 799  =  63 bp
21 amino acids  =  2.4 kDa
Product: leader sequence from mouse immunoglobulin κ light chain
HA
800 .. 826  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
HA
800 .. 826  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
FAPα2
842 .. 1561  =  720 bp
240 amino acids  =  ~24.3 kDa
Product: fluorogen-activating protein composed of a dimer of the L5-MG single-chain antibody fragment (Szent-Gyorgyi et al., 2008)
binds malachite green derivatives to produce red fluorescence
FAPα2
842 .. 1561  =  720 bp
240 amino acids  =  ~24.3 kDa
Product: fluorogen-activating protein composed of a dimer of the L5-MG single-chain antibody fragment (Szent-Gyorgyi et al., 2008)
binds malachite green derivatives to produce red fluorescence
Myc
1598 .. 1627  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
Myc
1598 .. 1627  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
PDGFRβ TM domain
1631 .. 1777  =  147 bp
49 amino acids  =  5.4 kDa
Product: transmembrane domain from platelet derived growth factor receptor beta
PDGFRβ TM domain
1631 .. 1777  =  147 bp
49 amino acids  =  5.4 kDa
Product: transmembrane domain from platelet derived growth factor receptor beta
AmpR
4600 .. 5460  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4600 .. 5391  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4600 .. 5460  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5392 .. 5460  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4600 .. 5460  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
3357 .. 4145  =  789 bp
262 amino acids  =  28.7 kDa
Product: aminoglycoside phosphotransferase
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
3357 .. 4145  =  789 bp
262 amino acids  =  28.7 kDa
Product: aminoglycoside phosphotransferase
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
2161 .. 2749  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2161 .. 2749  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
5592 .. 6047  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
5592 .. 6047  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
10 .. 389  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
10 .. 389  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
4180 .. 4540  =  361 bp
SV40 enhancer and early promoter
SV40 promoter
4180 .. 4540  =  361 bp
SV40 enhancer and early promoter
bGH poly(A) signal
1805 .. 2029  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
1805 .. 2029  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
390 .. 593  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
390 .. 593  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
5461 .. 5565  =  105 bp
AmpR promoter
5461 .. 5565  =  105 bp
HSV TK poly(A) signal
3078 .. 3125  =  48 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
3078 .. 3125  =  48 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
T7 promoter
638 .. 656  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
638 .. 656  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
4194 .. 4332  =  139 bp
SV40 origin of replication
SV40 ori
4194 .. 4332  =  139 bp
SV40 origin of replication
ORF:  737 .. 1780  =  1044 bp
ORF:  347 amino acids  =  ~36.2 kDa
ORF:  4730 .. 4996  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2898 .. 3131  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  3306 .. 3836  =  531 bp
ORF:  176 amino acids  =  19.6 kDa
ORF:  3593 .. 3973  =  381 bp
ORF:  126 amino acids  =  14.3 kDa
ORF:  1522 .. 1755  =  234 bp
ORF:  77 amino acids  =  ~8.7 kDa
ORF:  1795 .. 2103  =  309 bp
ORF:  102 amino acids  =  11.3 kDa
ORF:  2887 .. 3336  =  450 bp
ORF:  149 amino acids  =  16.3 kDa
ORF:  4600 .. 5460  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3357 .. 4145  =  789 bp
ORF:  262 amino acids  =  28.7 kDa
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