pRSET-EmGFP

Vector for expressing Emerald GFP in bacteria.

Sequence Author: Thermo Fisher (Invitrogen)

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No matches
BspQI - SapI (3526) NspI (3413) AflIII - PciI (3409) PspFI (3109) BseYI (3105) AlwNI (3000) AhdI (2521) ScaI (2040) BsaHI (1981) XmnI (1921) T7 promoter XbaI (47) NdeI (87) ATG 6xHis NheI (125) BmtI (129) enterokinase site BspDI * - ClaI * (184) BamHI (191) EcoRI (197) BtgI - NcoI (207) BseRI (239) PfoI * (489) BsrGI (918) AvaI - BsoBI - PaeR7I - XhoI (929) HindIII (935) BlpI (993) EcoO109I (1020) BspEI * (1070) NgoMIV (1273) NaeI (1275) BanII (1307) BsaAI (1378) DraIII (1381) PsiI (1506) pRSET-EmGFP 3600 bp
BspQI  (3526)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (3526)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
NspI  (3413)
1 site
R C A T G Y Y G T A C R
AflIII  (3409)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (3409)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PspFI  (3109)
1 site
C C C A G C G G G T C G
BseYI  (3105)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI  (3000)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (2521)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
ScaI  (2040)
1 site
A G T A C T T C A T G A
BsaHI  (1981)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
XmnI  (1921)
1 site
G A A N N N N T T C C T T N N N N A A G
XbaI  (47)
1 site
T C T A G A A G A T C T
NdeI  (87)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
NheI  (125)
1 site
G C T A G C C G A T C G
BmtI  (129)
1 site
G C T A G C C G A T C G
BspDI  (184)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (184)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
BamHI  (191)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (197)
1 site
G A A T T C C T T A A G
BtgI  (207)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (207)
1 site
C C A T G G G G T A C C
BseRI  (239)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
PfoI  (489)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BsrGI  (918)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AvaI  (929)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (929)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (929)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (929)
1 site
C T C G A G G A G C T C
HindIII  (935)
1 site
A A G C T T T T C G A A
BlpI  (993)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
EcoO109I  (1020)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BspEI  (1070)
1 site
T C C G G A A G G C C T
* Blocked by Dam methylation.
NgoMIV  (1273)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (1275)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
BanII  (1307)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BsaAI  (1378)
1 site
Y A C G T R R T G C A Y
DraIII  (1381)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (1506)
1 site
T T A T A A A A T A T T
AmpR
1734 .. 2594  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1734 .. 1802  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1734 .. 2594  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1803 .. 2594  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1734 .. 2594  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ATG
89 .. 91  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
89 .. 91  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
6xHis
101 .. 118  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
101 .. 118  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
T7 tag
122 .. 154  =  33 bp
11 amino acids  =  1.1 kDa
Product: epitope tag from a T7 major capsid protein
T7 tag
122 .. 154  =  33 bp
11 amino acids  =  1.1 kDa
Product: epitope tag from a T7 major capsid protein
enterokinase site
167 .. 181  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
enterokinase site
167 .. 181  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
EmGFP
209 .. 928  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 1:  
   209 .. 211  =  3 bp
   1 amino acid  =  149.2 Da
Product: Emerald GFP
mammalian codon-optimized
EmGFP
209 .. 928  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 2:  1a  
   212 .. 214  =  3 bp
   1 amino acid  =  117.1 Da
Product: Emerald GFP
mammalian codon-optimized
EmGFP
209 .. 928  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 3:  
   215 .. 928  =  714 bp
   237 amino acids  =  26.7 kDa
Product: Emerald GFP
mammalian codon-optimized
EmGFP
209 .. 928  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
Product: Emerald GFP
mammalian codon-optimized
ori
2765 .. 3353  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2765 .. 3353  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1148 .. 1603  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1148 .. 1603  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
AmpR promoter
1629 .. 1733  =  105 bp
AmpR promoter
1629 .. 1733  =  105 bp
T7 terminator
1004 .. 1051  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
1004 .. 1051  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 promoter
9 .. 27  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
9 .. 27  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  187 .. 942  =  756 bp
ORF:  251 amino acids  =  29.1 kDa
ORF:  89 .. 928  =  840 bp
ORF:  279 amino acids  =  31.5 kDa
ORF:  1734 .. 2594  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  2198 .. 2464  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Download pRSET-EmGFP.dna file

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