rsFusionRed1

FusionRed fluorescent protein derivative that shows reversible photoswitching with visible light.
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No matches
600 400 200 End (699) DraIII (648) NdeI (630) BsrBI (603) AcuI (583) BsaWI (536) EcoP15I (479) SphI (476) PasI (414) BmrI (388) BseRI (383) PfoI * (332) PshAI (326) BsaHI (302) AvaI - BsoBI (256) DraI (243) KflI - PpuMI - EcoO109I (197) MscI (178) EaeI (176) HincII (60) MslI (31) Start (0) rsFusionRed1 rsFusionRed1 699 bp
End  (699)
0 sites
DraIII  (648)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NdeI  (630)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BsrBI  (603)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
AcuI  (583)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaWI  (536)
1 site
W C C G G W W G G C C W

Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
EcoP15I  (479)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
SphI  (476)
1 site
G C A T G C C G T A C G
PasI  (414)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BmrI  (388)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
BseRI  (383)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
PfoI  (332)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
PshAI  (326)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BsaHI  (302)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
AvaI  (256)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (256)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
DraI  (243)
1 site
T T T A A A A A A T T T
KflI  (197)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (197)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
EcoO109I  (197)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
MscI  (178)
1 site
T G G C C A A C C G G T
EaeI  (176)
1 site
Y G G C C R R C C G G Y
HincII  (60)
1 site
G T Y R A C C A R Y T G
MslI  (31)
1 site
C A Y N N N N R T G G T R N N N N Y A C
Start  (0)
0 sites
rsFusionRed1
1 .. 699  =  699 bp
232 amino acids  =  25.6 kDa
Product: FusionRed fluorescent protein derivative that shows reversible photoswitching with visible light
rsFusionRed1
1 .. 699  =  699 bp
232 amino acids  =  25.6 kDa
Product: FusionRed fluorescent protein derivative that shows reversible photoswitching with visible light
ORF:  1 .. 699  =  699 bp
ORF:  232 amino acids  =  25.6 kDa
ORF:  1 .. 594  =  594 bp
ORF:  198 amino acids  =  21.3 kDa
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Download rsFusionRed1.dna file

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Individual Sequences & Maps

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