pGLuc-Basic 2

Mammalian promoter reporter vector encoding secreted Gaussia luciferase.

Sequence Author: New England Biolabs

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Luciferase Vectors | More Plasmid Sets
No matches
AatII (4957) SgrDI (4956) ZraI (4955) SspI (4839) ScaI (4515) TatI (4513) BglI (4155) BsaI (4096) AflIII - PciI (3142) BstZ17I (2763) BsmI (2711) PfoI (2566) BglII (12) EcoRI (20) EcoRV (32) PaeR7I - PspXI - XhoI (36) HindIII (45) Acc65I (51) KpnI (55) Eco53kI (59) SacI (61) BamHI (63) BbsI (152) NruI (181) SacII (207) NotI (635) XbaI (691) DraIII (1057) CsiI - SexAI * (1348) BseRI (1577) StuI (1580) AvrII (1581) TspMI - XmaI (1602) SmaI (1604) BclI * (1632) KasI (1790) NarI (1791) SfoI (1792) PluTI (1794) PflFI - Tth111I (1909) BssHII (2188) RsrII (2307) BstBI (2473) pGLuc-Basic 2 4958 bp
AatII  (4957)
1 site
G A C G T C C T G C A G
SgrDI  (4956)
1 site
C G T C G A C G G C A G C T G C
ZraI  (4955)
1 site
G A C G T C C T G C A G
SspI  (4839)
1 site
A A T A T T T T A T A A
ScaI  (4515)
1 site
A G T A C T T C A T G A
TatI  (4513)
1 site
W G T A C W W C A T G W
BglI  (4155)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsaI  (4096)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AflIII  (3142)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (3142)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstZ17I  (2763)
1 site
G T A T A C C A T A T G
BsmI  (2711)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
PfoI  (2566)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BglII  (12)
1 site
A G A T C T T C T A G A
EcoRI  (20)
1 site
G A A T T C C T T A A G
EcoRV  (32)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PaeR7I  (36)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (36)
1 site
V C T C G A G B B G A G C T C V
XhoI  (36)
1 site
C T C G A G G A G C T C
HindIII  (45)
1 site
A A G C T T T T C G A A
Acc65I  (51)
1 site
G G T A C C C C A T G G
KpnI  (55)
1 site
G G T A C C C C A T G G
Eco53kI  (59)
1 site
G A G C T C C T C G A G
SacI  (61)
1 site
G A G C T C C T C G A G
BamHI  (63)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BbsI  (152)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
NruI  (181)
1 site
T C G C G A A G C G C T
SacII  (207)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
NotI  (635)
1 site
G C G G C C G C C G C C G G C G
XbaI  (691)
1 site
T C T A G A A G A T C T
DraIII  (1057)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
CsiI  (1348)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (1348)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BseRI  (1577)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
StuI  (1580)
1 site
A G G C C T T C C G G A
AvrII  (1581)
1 site
C C T A G G G G A T C C
TspMI  (1602)
1 site
C C C G G G G G G C C C
XmaI  (1602)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (1604)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BclI  (1632)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
KasI  (1790)
1 site
G G C G C C C C G C G G
NarI  (1791)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1792)
1 site
G G C G C C C C G C G G
PluTI  (1794)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
PflFI  (1909)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1909)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BssHII  (2188)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
RsrII  (2307)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstBI  (2473)
1 site
T T C G A A A A G C T T
AmpR
3962 .. 4822  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3962 .. 4753  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3962 .. 4822  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4754 .. 4822  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3962 .. 4822  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
1663 .. 2457  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
NeoR/KanR
1663 .. 2457  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
ori
3203 .. 3791  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3203 .. 3791  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
hGLuc
76 .. 633  =  558 bp
185 amino acids  =  19.9 kDa
Product: secreted Gaussia luciferase
human codon-optimized
hGLuc
76 .. 633  =  558 bp
185 amino acids  =  19.9 kDa
Product: secreted Gaussia luciferase
human codon-optimized
f1 ori
824 .. 1252  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
824 .. 1252  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 promoter
1266 .. 1596  =  331 bp
SV40 enhancer and early promoter
SV40 promoter
1266 .. 1596  =  331 bp
SV40 enhancer and early promoter
SV40 poly(A) signal
2631 .. 2752  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2631 .. 2752  =  122 bp
SV40 polyadenylation signal
AmpR promoter
4823 .. 4927  =  105 bp
AmpR promoter
4823 .. 4927  =  105 bp
MCS
12 .. 68  =  57 bp
multiple cloning site
MCS
12 .. 68  =  57 bp
multiple cloning site
poly(A) signal
642 .. 690  =  49 bp
synthetic polyadenylation signal
poly(A) signal
642 .. 690  =  49 bp
synthetic polyadenylation signal
SV40 ori
1447 .. 1582  =  136 bp
SV40 origin of replication
SV40 ori
1447 .. 1582  =  136 bp
SV40 origin of replication
ORF:  76 .. 633  =  558 bp
ORF:  185 amino acids  =  19.9 kDa
ORF:  775 .. 1041  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  1663 .. 2457  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  1835 .. 2221  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  4092 .. 4358  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  4898 .. 344  =  405 bp
ORF:  134 amino acids  =  14.3 kDa
ORF:  3962 .. 4822  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  4719 .. 753  =  993 bp
ORF:  330 amino acids  =  36.5 kDa
ORF:  1972 .. 2508  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
Click here to try SnapGene

Download pGLuc-Basic 2.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.